冷冻保存会改变水牛精子的运动学和线粒体参数、顶体和细胞内钙状态。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-01 DOI:10.54680/fr24410110612

D. Kumar, J. S. Mehta, A. Jerome, P. Kumar, D. Kumar, S. Bhardwaj, C. Patil, R. Bala, N. Verma, EmptyYN Y Satish, M. Vimani, R. K. Sharma, P. Singh

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引用次数: 0

摘要

目的研究三个季节（冬季、舒适季节和夏季）和冷冻保存对水牛精子运动学和功能特性的影响。结果新鲜精子的总运动（TM）、渐进运动（PM）和快速运动（RM）与冷冻解冻精子相比更高（P < 0.05）。同样，所有运动学参数[即平均路径速度（VAP）、直线速度（VSL）、曲线速度（VCL）、节拍交叉频率（BCF）、侧头位移（ALH）、线性度（LIN）和直线度（STR）]在新鲜阶段都更高（P < 0.01）。就季节而言，使用冬季采集的精子时，冷冻解冻精液的TM（57.67 ± 115 %）、PM（50.2 ± 1.15 %）和RM（51.6 ± 1.19 %）较高（P < 0.01）。冷冻保存阶段（即平衡和冻融）对线粒体超氧化物阳性状态（MSPS）、线粒体膜电位（MMP）、顶体状态和细胞内钙状态也有显著影响（P < 0.01）。Doi.org/10.54680/fr24410110612.

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Cryopreservation alters buffalo sperm kinematics and mitochondrial parameters, acrosome and intra-cellular calcium status.

BACKGROUND Little is known about the effects of different seasons on the cryopreservation success of buffalo sperm in terms of kinematics and sperm functional parameters. OBJECTIVE To study the effect of three seasons (winter, comfort and summer) and cryopreservation on sperm kinematics and functional properties in buffalo bulls. MATERIALS AND METHODS Semen ejaculates (n = 90) collected during three seasons i.e. winter (n = 30), comfort (n = 30), summer (n = 30) were evaluated for sperm kinematics and functional properties. RESULTS Sperm kinematics with respect to total (TM), progressive (PM) and rapid motility (RM) was higher (P < 0.05) in fresh sperm compared to sperm that had been frozen-thawed. Similarly, all kinematic parameters [viz. average path velocity (VAP), straight linear velocity (VSL), curvilinear velocity (VCL), beats cross frequency (BCF), lateral head displacement (ALH), linearity (LIN) and straightness (STR)] were higher (P < 0.01) at the fresh stage. With respect to season, frozen-thawed semen TM (57.67 ± 115 %), PM (50.2 ± 1.15 %) and RM (51.6 ± 1.19 %) were higher (P < 0.01) when using sperm collected during winter. The stage of cryopreservation (i.e., equilibration and freeze-thawing) also showed significant effects (P < 0.01) on mitochondrial superoxide positive status (MSPS), mitochondrial membrane potential (MMP), acrosome status and intra-cellular calcium status. CONCLUSION The season of sperm collection and cryopreservation have significant effects on buffalo bull sperm kinematics and functional properties. Doi.org/10.54680/fr24410110612.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.