{"title":"科里拉金功能化脱细胞细胞外基质作为人造血管,通过清除活性氧改善内皮化和抗炎作用","authors":"Xu Wang, Hanmei Fu, Huibin Wu, Xiaohua Peng, Xu Peng, Xixun Yu, Hui Liu, Junmei Wu, Ling Luo, Shan Yan, Xinglin Cheng, Xiong Zhou, Xiangyang Yuan","doi":"10.1093/rb/rbae074","DOIUrl":null,"url":null,"abstract":"\n The performance of biological-originated blood vessels in clinical remains disappointing due to fast occlusion caused by acute thrombosis or long-standing inflammation. How to prevent rapid degradation and inhibit acute inflammation but maintain their high bioactivity is still a significant challenge. As a bioactive polyphenol in various traditional Chinese medicine, Corilagin (Cor) exhibits excellent anticoagulant, anti-inflammatory, and rapid ROS consumption properties. Inspired by abundant supramolecular interactions in organisms, we selected it to crosslink tissues via purely H-bonds to simulate these natural interactions without introducing potential toxic aldehyde or carboxyl groups. Results show that 2 mg/mL was selected as the optimal corilagin concentration to form a stable crosslinking network (FI > 95%) and effectively delay their degradation. Corilagin modification not only enhances ECs adhesion and monolayer function via accelerating VEGF and TGF-β secretion but also promotes macrophage transformation from pro-inflammatory M1 phenotype to anti-inflammatory M2 ones. In vitro and ex-vivo studies implied that corilagin-crosslinked samples exhibited low platelet accumulation and decreased thrombin generation. In vivo evaluation further confirmed that corilagin-introducing could effectively consume ROS, thus exhibiting rapid endothelialization, suppressed inflammation, and reduced mineral deposition. Overall, corilagin crosslinking provided a bright future for blood vessels’ long-term patency and adapted to various blood-contacting surfaces.","PeriodicalId":5,"journal":{"name":"ACS Applied Materials & Interfaces","volume":"19 17","pages":""},"PeriodicalIF":8.3000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Corilagin functionalized decellularized extracellular matrix as artificial blood vessels with improved endothelialization and anti-inflammation by reactive oxygen species scavenging\",\"authors\":\"Xu Wang, Hanmei Fu, Huibin Wu, Xiaohua Peng, Xu Peng, Xixun Yu, Hui Liu, Junmei Wu, Ling Luo, Shan Yan, Xinglin Cheng, Xiong Zhou, Xiangyang Yuan\",\"doi\":\"10.1093/rb/rbae074\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n The performance of biological-originated blood vessels in clinical remains disappointing due to fast occlusion caused by acute thrombosis or long-standing inflammation. How to prevent rapid degradation and inhibit acute inflammation but maintain their high bioactivity is still a significant challenge. As a bioactive polyphenol in various traditional Chinese medicine, Corilagin (Cor) exhibits excellent anticoagulant, anti-inflammatory, and rapid ROS consumption properties. Inspired by abundant supramolecular interactions in organisms, we selected it to crosslink tissues via purely H-bonds to simulate these natural interactions without introducing potential toxic aldehyde or carboxyl groups. Results show that 2 mg/mL was selected as the optimal corilagin concentration to form a stable crosslinking network (FI > 95%) and effectively delay their degradation. Corilagin modification not only enhances ECs adhesion and monolayer function via accelerating VEGF and TGF-β secretion but also promotes macrophage transformation from pro-inflammatory M1 phenotype to anti-inflammatory M2 ones. In vitro and ex-vivo studies implied that corilagin-crosslinked samples exhibited low platelet accumulation and decreased thrombin generation. In vivo evaluation further confirmed that corilagin-introducing could effectively consume ROS, thus exhibiting rapid endothelialization, suppressed inflammation, and reduced mineral deposition. Overall, corilagin crosslinking provided a bright future for blood vessels’ long-term patency and adapted to various blood-contacting surfaces.\",\"PeriodicalId\":5,\"journal\":{\"name\":\"ACS Applied Materials & Interfaces\",\"volume\":\"19 17\",\"pages\":\"\"},\"PeriodicalIF\":8.3000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Materials & Interfaces\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1093/rb/rbae074\",\"RegionNum\":2,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MATERIALS SCIENCE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Materials & Interfaces","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1093/rb/rbae074","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
Corilagin functionalized decellularized extracellular matrix as artificial blood vessels with improved endothelialization and anti-inflammation by reactive oxygen species scavenging
The performance of biological-originated blood vessels in clinical remains disappointing due to fast occlusion caused by acute thrombosis or long-standing inflammation. How to prevent rapid degradation and inhibit acute inflammation but maintain their high bioactivity is still a significant challenge. As a bioactive polyphenol in various traditional Chinese medicine, Corilagin (Cor) exhibits excellent anticoagulant, anti-inflammatory, and rapid ROS consumption properties. Inspired by abundant supramolecular interactions in organisms, we selected it to crosslink tissues via purely H-bonds to simulate these natural interactions without introducing potential toxic aldehyde or carboxyl groups. Results show that 2 mg/mL was selected as the optimal corilagin concentration to form a stable crosslinking network (FI > 95%) and effectively delay their degradation. Corilagin modification not only enhances ECs adhesion and monolayer function via accelerating VEGF and TGF-β secretion but also promotes macrophage transformation from pro-inflammatory M1 phenotype to anti-inflammatory M2 ones. In vitro and ex-vivo studies implied that corilagin-crosslinked samples exhibited low platelet accumulation and decreased thrombin generation. In vivo evaluation further confirmed that corilagin-introducing could effectively consume ROS, thus exhibiting rapid endothelialization, suppressed inflammation, and reduced mineral deposition. Overall, corilagin crosslinking provided a bright future for blood vessels’ long-term patency and adapted to various blood-contacting surfaces.
期刊介绍:
ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.