Differential impact of chronic intermittent hypoxia and stress changes on condylar development

Objectives

This study aimed to determine the effects of chronic intermittent hypoxia (CIH) and stress change (SC) on the development of the condyle in mouth breathing rats.

Design

A total of 120 4-week-old rats were randomly assigned to one of five groups. The control (Ctrl) group was the blank control and the intermittent nasal obstruction (INO) group was the positive control. Mild CIH (mCIH) and severe CIH (sCIH) groups were developed by adjusting environmental oxygen concentration and monitoring real-time blood oxygen saturation (SpO₂). The SC group was developed using INO, increased environmental oxygen concentration, and real-time SpO₂ monitoring. Six rats from each group were sacrificed for analysis at 0, 1, 2, or 4 weeks.

Results

Similar to the INO group, condyle and mandibular body development in the sCIH group, but not in the mCIH group, was significantly inhibited compared with the Ctrl group. The SC group had inhibited development of the condyle, especially of the posterior zone, but had minimal impact on the growth of the mandible.

Conclusion

The inhibitory effects of CIH on the development of the condyle and mandibular body were SpO₂-dose-dependent. When SC occurred, inhibited development was observed in the posterior zone of condyle but not the whole mandible. These findings provide important insights for targeted interventions that address the consequences of mouth breathing in children.