研究衰老细胞分泌组的方法

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS
Afshin Samiminemati, Domenico Aprile, Dario Siniscalco, G. Di Bernardo
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引用次数: 0

摘要

分泌组 "一词最初用于描述细胞在不同情况下分泌的蛋白质,但最近的研究证明,分泌组中还存在其他分子,如核糖核酸和化合物。对分泌物组的研究对疾病的诊断和治疗具有重要意义,因为它能让人们深入了解细胞的功能,包括免疫反应、发育和稳态。细胞衰老通过停止细胞分裂,分泌称为衰老相关分泌表型(SASP)的物质,在癌症防御和衰老中发挥作用。有多种技术可用于分析分泌组:基于蛋白质的方法,如质谱法和蛋白质微阵列;基于核酸的方法,如 RNA 测序、微阵列和硅预测。每种方法在描述分泌分子特征方面都有其独特的优势和局限性。质谱技术使自上而下和自下而上彻底分析分泌组的策略成为可能。这些方法有助于了解细胞功能、疾病原因和适当的治疗目标。本综述将讨论它们的方法、优点和缺点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Methods to Investigate the Secretome of Senescent Cells
The word “secretome” was first used to describe the proteins that cells secrete under different circumstances; however, recent studies have proven the existence of other molecules such as RNA and chemical compounds in the secretome. The study of secretome has significance for the diagnosis and treatment of disease as it provides insight into cellular functions, including immune responses, development, and homeostasis. By halting cell division, cellular senescence plays a role in both cancer defense and aging by secreting substances known as senescence-associated secretory phenotypes (SASP). A variety of techniques could be used to analyze the secretome: protein-based approaches like mass spectrometry and protein microarrays, nucleic acid-based methods like RNA sequencing, microarrays, and in silico prediction. Each method offers unique advantages and limitations in characterizing secreted molecules. Top-down and bottom-up strategies for thorough secretome analysis are became possible by mass spectrometry. Understanding cellular function, disease causes, and proper treatment targets is aided by these methodologies. Their approaches, benefits, and drawbacks will all be discussed in this review.
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来源期刊
Methods and Protocols
Methods and Protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)
CiteScore
3.60
自引率
0.00%
发文量
85
审稿时长
8 weeks
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