Effect of hydrolat from Melaleuca alternifolia (Maiden & Betche) Cheel leaves in rats skin wound healing

Melaleuca alternifolia (Maiden & Betche) Cheel was introduced in Brazil from Australia and is commonly known as the tea tree. The trees reach 5m in height, growing in sandy soil with low water retention capacity. The objectives of the present study were to identify the species cultivated, analyze tea tree oil and hydrosol, and evaluate the effect of hydrosol from its leaves on skin wound healing in rats. Melaleuca samples were obtained from Sitio Melaleuca, Ibiuna, São Paulo, and the oil was obtained using steam drag distillation technique, with the posterior collection of hydrosols containing 3 to 5% oil. A gel with 10% hydrosol was prepared. Thirty rats (Ethics Committee UNINOVE AN 37/2014) were separated into three groups: one group was treated with 1mL of hydrosol gel, another group with 1mL of distilled water/gel (negative control), and the third group with a thin layer of fibrinase® (positive control). Daily application was performed on a 4cm2 square wound in the animal dorsal region and observed for 14 days. Results indicated a statistical difference in the wound areas of animals treated with hydrosol from Melaleuca gel and the control animals. At the end of 14 days, the wounds of animals treated with hydrosol gel were fully healed, corresponding to 91.3% better response when compared to the control group. Animals treated with fibrinase® achieved only 63.9% more than the control animals. Conclusion: Tea tree (Melaleuca alternifolia (Maiden & Betche) Cheel) hydrosol can help with skin healing and can be used as an alternative treatment for healing wounds in humans at a low cost.