油茶光周期调控开花关键因子 CoGI 的表达分析和互作蛋白筛选

Lemei Juan, Shuangshuang Ren, Qian Liu, Liling Zhang, Jindong Yan, Jian’an Li
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摘要

光周期是油茶(Camellia oleifera Abel.(油茶)开花的关键调节因子。GIGANTEA(GI)是一个关键的调节因子,它不仅协调着复杂的昼夜节律,而且还管理着依赖光周期的开花。为了探索油菜中 GI(CoGI)的功能,我们获得了 CoGI 基因编码序列,并利用生物信息学分析了 CoGI 蛋白序列。此外,我们还对 CoGI 进行了时空表达分析。并利用酵母双杂交实验筛选了CoGI的相互作用蛋白。进化分析表明,CoGI蛋白具有高度的保守性,它与来自山茶的GI蛋白(CsGI)聚类在一个共同的进化分支上。CoGI在各部位的表达量不同,组织表达分析表明,CoGI基因在油茶叶片中的相对表达量最高,而在种皮中的表达量最低。过表达 CoGI 的转基因拟南芥(Arabidopsis thaliana)在长日照条件下会提前开花。此外,酵母双杂交文库筛选发现了 7 个 C. oleifera 蛋白与 CoGI 之间的相互作用:CoACR9、CoLAO、CoDExH12-like、CoIT1K-like、CoUPF0481、CoIDM3 和 CoAt4g27190-like。研究结果表明,CoGI 对油橄榄开花至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression Analysis and Interaction Protein Screening of CoGI, the Key Factor in Photoperiod Regulation of Flowering in Camellia oleifera Abel
Photoperiod is a pivotal regulatory factor in the flowering of Camellia oleifera Abel. (C. oleifera). GIGANTEA (GI) serves as a pivotal regulator, not only orchestrating the intricate circadian rhythm but also governing photoperiod-dependent flowering. In order to explore the function of GI in C. oleifera (CoGI), we obtained a CoGI gene-coding sequence and analyzed a CoGI protein sequence using bioinformatics. Furthermore, we conducted a spatiotemporal expression analysis of CoGI. And a yeast two-hybridization assay was used to screen the interacting proteins of CoGI. Evolutionary analysis revealed high conservation of the CoGI protein, which clustered with the GI protein from Camellia sinensis (CsGI) on a common evolutionary branch. The expression of CoGI was different in each part, and a tissue expression analysis revealed that the relative expression level of the CoGI gene is highest in the leaves of C. oleifera, while it is at its lowest in the seed coats. Transgenic Arabidopsis thaliana (Arabidopsis) overexpressing CoGI exhibited early flowering under long-day conditions. In addition, the yeast two-hybrid library screening revealed interactions between seven C. oleifera proteins and CoGI: CoACR9, CoLAO, CoDExH12-like, CoIT1K-like, CoUPF0481, CoIDM3, and CoAt4g27190-like. The findings demonstrated that CoGI is crucial to C. oleifera’s flowering.
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