转录组和代谢组分析为草莓(Fragaria × ananassa Duch.)应对干旱胁迫提供了新见解

Lili Jiang, Ruimin Song, Xiaofang Wang, Jie Wang, Chong Wu
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摘要

草莓植株根浅叶大,对水位变化高度敏感。为探索草莓对水分胁迫响应的理化和分子机制,为草莓科学灌溉提供新思路,我们利用植物芯片系统测量了盆栽 "张记 "草莓植株在干旱和涝害处理下的蒸腾速率、鲜重、生物量增加等指标。对轻度干旱、中度干旱、严重干旱和补水处理后的草莓叶片进行转录组和代谢组分析,以确定参与干旱胁迫响应的关键基因和代谢产物。在一定阈值以下,亏缺灌溉处理后草莓植株的蒸腾速率明显低于常规水处理。转录组分析表明,涉及氧化还原酶活性、硫和氮代谢的基因以及淀粉和蔗糖的基因都被上调。草莓植物在干旱胁迫下会分泌多种内源生长激素以维持正常生长。在轻度和中度干旱处理中,水杨酸(SA)和脱落酸(ABA)的合成受到上调。然而,1-氨基环丙烷羧酸(ACC)和吲哚-3-乙酸(IAA)的合成在严重干旱处理中下调,而在严重干旱处理后的复水处理中上调。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcriptomic and Metabolomic Analyses Provide New Insights into the Response of Strawberry (Fragaria × ananassa Duch.) to Drought Stress
Strawberry plants have shallow roots and large leaves, which are highly sensitive to variations in water levels. To explore the physicochemical and molecular mechanisms of strawberry response to water stress, and provide new ideas for strawberry scientific irrigation, we measured the transpiration rate, fresh weight, biomass gain, and other indicators of potted “Zhangji” strawberry plants under drought and waterlogging treatments using a Plantarray system. Transcriptomic and metabolomic analyses of strawberry leaves following mild drought, moderate drought, severe drought, and rehydration treatments were performed to identify key genes and metabolites involved in the response to drought stress. Below a certain threshold, the transpiration rate of strawberry plants was significantly lower after the deficit irrigation treatment than the conventional water treatment. Transcriptome analysis revealed that genes involved in oxidoreductase activity and in sulfur and nitrogen metabolism were up-regulated, as well as starch and sucrose. Strawberry plants secrete various endogenous growth hormones to maintain their normal growth under drought stress. The syntheses of salicylic acid (SA) and abscisic acid (ABA) were up-regulated in the mild and moderate drought treatments. However, the syntheses of 1-aminocyclopropanecarboxylic acid (ACC) and indole-3-acetic acid (IAA) were down-regulated in severe drought treatment and up-regulated in rehydration after severe drought treatment.
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