增强细菌性脑膜炎脑脊液的细胞学特征和诊断意义

IF 2.5 4区 医学 Q2 PATHOLOGY
Yong Long, Yufen Peng, Zhaojun Huang, Min Zhu, Chenyi Wan
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引用次数: 0

摘要

本研究旨在探讨细菌性脑膜炎(BM)患者脑脊液(CSF)的细胞学特征和诊断意义。回顾性分析了临床、头颅影像学、CSF-下一代测序、CSF检查和CSF细胞学数据。脑脊液细胞学样本使用脑脊液细胞造粒机(沉淀法)制备,并使用 May-Grunwald-Glemsa (MGG) 法染色。八名患者(四男四女)年龄在 41-67 岁之间。其中,2 名患者在过去 4 个月内接受过脑部手术,1 名患者有 8 年中耳炎病史,2 名患者有突发牙痛病史。临床表现包括发热、头痛、突发意识障碍和颈部僵硬。7 名患者的脑脊液细胞学检查发现以中性粒细胞为主的异常炎症变化。常规 CSF 细胞计数超过 100/uL 的病例仅有 4 例,这表明 CSF 细胞学检测 CSF 炎症反应的阳性率高于常规细胞计数。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhancing the cytological features and diagnostic significance of cerebrospinal fluid in bacterial meningitis
The objective of this study was to investigate the cytological features and diagnostic significance of cerebrospinal fluid (CSF) in bacterial meningitis (BM). Patients diagnosed with BM at the First Affiliated Hospital of Nanchang University Hospital between August 2021 and April 2022 were enrolled. Clinical, cranial imaging, CSF-next-generation sequencing, CSF examination, and CSF cytology data were retrospectively analyzed. CSF cytology samples were prepared using a CSF cell pelletizer (precipitation method) and stained using the May–Grunwald–Glemsa (MGG) method. The χ2 test was employed to compare the positive rate of routine CSF count and CSF cytology. Eight patients (four males and four females), aged 41–67 years, were included. Among them, two patients had undergone brain surgery within the past 4 months, one patient had an 8-year history of otitis media, and two patients had a history of sudden toothache. Clinical manifestations included fever, headache, sudden disturbance of consciousness, and neck stiffness. CSF cytology revealed abnormal inflammatory changes dominated by neutrophils in seven patients. Routine CSF cell counts exceeded 100/uL in only four cases, indicating a higher positive rate of CSF cytology for detecting CSF inflammatory reactions compared to routine cell count. Comparative detection of bacteria through the observation of CSF cytology inflammatory status in BM patients are more useful for diagnosing BM than routine CSF counts.
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来源期刊
Cytojournal
Cytojournal PATHOLOGY-
CiteScore
2.20
自引率
42.10%
发文量
56
审稿时长
>12 weeks
期刊介绍: The CytoJournal is an open-access peer-reviewed journal committed to publishing high-quality articles in the field of Diagnostic Cytopathology including Molecular aspects. The journal is owned by the Cytopathology Foundation and published by the Scientific Scholar.
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