具有增强过氧化物酶样活性的介孔 Cu2O-CuO/O-g-C3N4 纳米复合材料,用于比色 H2O2 传感

IF 2.8 3区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Shemeena Mullakkattuthodi, Binitha N. Narayanan
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引用次数: 0

摘要

在这里,Cu2O-CuO 与氧掺杂的 g-C3N4 被用于通过氧化 3,3',5,5'-四甲基联苯胺(TMB)来比色感应 H2O2。通过 XPS、XRD、傅立叶变换红外光谱等进行的材料表征研究证明了 g-C3N4 上同时存在 Cu2O 和 CuO 以及氧的掺杂。在制备过程中使用柠檬酸可使 g-C3N4 形成介孔结构并掺入氧。这种铜掺杂的剥离 g-C3N4 纳米酶具有较高的过氧化物酶样活性,这是因为它具有更小的带隙、多孔性和 g-C3N4 中掺氧等改良特性,因而电子迁移和转移速度快。研究采用了 Michaelis-Menten 机制来研究动力学,结果发现所获得的 Km 值和 Vmax 值与已报道的研究结果相符。通过机理研究,确定了参与 TMB 氧化的活性氧为氧超氧自由基阴离子(-O2-)。感应的线性范围为 2.5-250 µM,检测限(LOD)为 1 µM H2O2。该纳米酶在 H2O2 检测中显示出最小的干扰量和良好的重复使用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Mesoporous Cu2O–CuO/O–g-C3N4 nanocomposite with enhanced peroxidase-like activity for the colorimetric H2O2 sensing

Mesoporous Cu2O–CuO/O–g-C3N4 nanocomposite with enhanced peroxidase-like activity for the colorimetric H2O2 sensing

Herein, Cu2O–CuO incorporated oxygen-doped g-C3N4 has been utilized for the colorimetric sensing of H2O2 by the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB). The material characterization studies via XPS, XRD, FTIR spectroscopy etc. proved the presence of both Cu2O and CuO as well as the doping of oxygen on g-C3N4. The use of citric acid in the preparation led to a mesoporous architecture together with oxygen doping to g-C3N4. The high peroxidase-like activity of the present Cu-incorporated exfoliated g-C3N4 nanoenzyme aroused from the improved features such as smaller band gap, porous nature, oxygen doping to g-C3N4, and thus resulted fast electron mobility and transfer. Michaelis–Menten mechanism is used to study the kinetics, where the obtained Km and Vmax values are found to be relevant in comparison with the reported studies. From the mechanistic investigation, the reactive oxygen species involved in the TMB oxidation is ascertained as oxygen superoxide radical anion (O2). The linear range in sensing is 2.5–250 µM with a limit of detection (LOD) of 1 µM H2O2. The nanoenzyme showed the least amount of interference and a promising reusability in H2O2 sensing.

Graphical abstract

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来源期刊
CiteScore
5.70
自引率
18.20%
发文量
229
审稿时长
2.6 months
期刊介绍: Research on Chemical Intermediates publishes current research articles and concise dynamic reviews on the properties, structures and reactivities of intermediate species in all the various domains of chemistry. The journal also contains articles in related disciplines such as spectroscopy, molecular biology and biochemistry, atmospheric and environmental sciences, catalysis, photochemistry and photophysics. In addition, special issues dedicated to specific topics in the field are regularly published.
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