用于心肌再生的MWCNT负载PCL/PXS-PCL双层心脏贴片:体外和体内研究

IF 4.7 3区 工程技术 Q2 ENGINEERING, ENVIRONMENTAL
Faraz Sigaroodi, Safieh Boroumand, Mahya Rahmani, Shahram Rabbani, Simzar Hosseinzadeh, Masoud Soleimani, Mohammad-Mehdi Khani
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引用次数: 0

摘要

最近在开发用于心肌再生的心脏贴片方面取得的进展为心肌梗塞(MI)后的治疗带来了新的希望。在此,我们介绍了一种由聚己内酯(PCL)、聚(木糖醇癸二酸酯)(PXS)和多壁碳纳米管(MWCNTs)组成的新型双层纳米纤维心脏贴片。首先,我们电纺了不同的单层支架,包括 PCL、PCL/MWCNT、PCL/PXS 和 PCL/PXS/MWCNT,并对其物理、机械和生物性能进行了表征,以确定不同材料成分对其支架性能的相互作用效应。体外试验证实了 PXS 和 MWCNT 与 PCL 混合后的协同效应,从而制造出具有良好生物相容性的导电且组织良好的纳米纤维。随后,一种由 PCL/PXS/MWCNT 纳米纤维电纺在 PCL 纤维层上组成的双层纳米纤维支架被制造出来,从而获得了一种能够提供心脏贴片理想特性的高效结构。双层性质提高了 PCL/PXS/MWCNT 单层的机械性能,同时保持了其适当的润湿性和可接受的导电性。在活/死试验中观察到,双层支架上的 H9c2 细胞具有极佳的存活能力和增殖能力。此外,细胞与基质的相互作用证实,双层纳米纤维可减少播种的 NIH3T3 细胞的肌成纤维细胞分化,这可能有利于心肌梗死后的心脏修复。将双层纳米纤维移植到心肌梗死大鼠的梗死心脏上 4 周后,缺血区缩小,心脏功能明显改善,而且观察到巨噬细胞的活化程度非常轻微。这些研究结果表明,含有 PXS 的纳米纤维心脏贴片可用于心肌梗死后的心肌修复,具有潜在的持久性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

MWCNT-Loaded PCL/PXS-PCL Bilayer Cardiac Patch for Myocardial Regeneration: An In Vitro and In Vivo Study

MWCNT-Loaded PCL/PXS-PCL Bilayer Cardiac Patch for Myocardial Regeneration: An In Vitro and In Vivo Study

Recent progress in developing cardiac patches for regenerating the myocardium has opened a new hope after myocardial infarction (MI). Herein, we introduce a novel bilayer nanofiber cardiac patch composed of polycaprolactone (PCL), poly(xylitol sebacate) (PXS), and multi-walled carbon nanotubes (MWCNTs). First, we electrospun different monolayer scaffolds, including PCL, PCL/MWCNT, PCL/PXS, and PCL/PXS/MWCNTs, and characterized their physical, mechanical, and biological performance to determine the interaction effects of different material compositions on their scaffold properties. In vitro examinations confirmed the cooperative effect of PXS and MWCNT in blending with PCL to fabricate conductive and well-organized nanofibers with good biocompatibility. Subsequently, a bilayer nanofiber scaffold composed of PCL/PXS/MWCNT nanofibers electrospun over a PCL fibrous layer was fabricated to achieve an efficient structure capable of providing the desirable characteristics of a cardiac patch. The bilayer nature increased the mechanical performance of the PCL/PXS/MWCNT monolayer while preserving its appropriate wettability and acceptable conductivity. Excellent viability and proliferation of H9c2 cells on the bilayer scaffolds were observed in the live/dead assay. Moreover, cell-matrix interaction confirmed that bilayer nanofibers decrease myofibroblast differentiation of seeded NIH3T3 cells, which may be beneficial for cardiac repair post-MI. After transplantation of the bilayer nanofiber onto the infarcted heart of the MI rats for 4 weeks, the ischemic zone decreased, cardiac function significantly improved and very slightly activated macrophages were observed. These findings suggested a potentially durable nanofiber cardiac patch containing PXS for myocardial repair post-MI.

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来源期刊
Journal of Polymers and the Environment
Journal of Polymers and the Environment 工程技术-高分子科学
CiteScore
9.50
自引率
7.50%
发文量
297
审稿时长
9 months
期刊介绍: The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.
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