Angela Cristina Molon, Daniele Heguedusch, Fabio Daumas Nunes, Rebeca Boltes Cecatto, Adriana Lino dos Santos Franco, Camila de Oliveira Rodini Pegoraro, Maria Fernanda Setúbal Destro Rodrigues
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引用次数: 0
摘要
口腔鳞状细胞癌(OSCC)占口腔癌的 90% 以上,以侵袭性强、预后差而闻名。光动力疗法(PDT)已成为一种前景广阔的辅助疗法,它与免疫性细胞死亡有关,能激活先天性和适应性抗肿瘤反应。自然杀伤(NK)细胞是消灭恶性细胞的关键角色,但在光动力疗法中尚未得到广泛研究。本研究评估了光动力疗法是否会增加 OSCC 细胞系对 NK 细胞毒性的敏感性。研究人员使用 5-aminolevulinic acid(5-ALA)和 LED 照射对 Ca1 和 Luc4 细胞系进行了光动力疗法。结果显示,PDT 后细胞存活率的下降呈剂量依赖性。基因表达分析表明,Ca1 中的 NK 细胞激活配体(ULBP1-4、MICA/B)上调,MHC I 类表达减少,表明 NK 细胞易感性增加。在 Ca1 细胞中,NK 细胞的细胞毒性增强得到了证实,而在 Luc4 细胞中则没有得到证实。这些研究结果表明,PDT 可增强 OSCC 中 NK 细胞介导的细胞毒性,为改进治疗策略提供了潜力。
Oral squamous cell carcinoma (OSCC) constitutes over 90% of oral cancers, known for its aggressiveness and poor prognosis. Photodynamic therapy (PDT) has emerged as a promising adjuvant therapy and is linked to immunogenic cell death, activating innate and adaptive anti-tumor responses. Natural Killer (NK) cells, key players in malignant cell elimination, have not been extensively studied in PDT. This study evaluates whether PDT increases OSCC cell lines' susceptibility to NK cell cytotoxicity. PDT, using 5-aminolevulinic acid (5-ALA) and LED irradiation, was applied to Ca1 and Luc4 cell lines. Results showed a dose-dependent viability decrease post-PDT. Gene expression analysis revealed upregulation of NK cell-activating ligands (ULBP1-4, MICA/B) and decreased MHC class I expression in Ca1, suggesting increased NK cell susceptibility. Enhanced NK cell cytotoxicity was confirmed in Ca1 but not in Luc4 cells. These findings indicate that PDT may enhance NK cell-mediated cytotoxicity in OSCC, offering potential for improved treatment strategies.
期刊介绍:
The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.