推进咳嗽研究：豚鼠模型咳嗽挑战中使用双腔与全身褶压计的方法论启示

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-16 DOI:10.1016/j.resp.2024.104302

Jana Plevkova , Janka Jakusova , Mariana Brozmanova , Zuzana Biringerova , Tomas Buday

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引用次数: 0

摘要

目的：本研究比较了全身胸透（WBP）和双室胸透（DCP）两种柠檬酸诱导豚鼠咳嗽的方法，以评估其有效性：方法：16 只无特定病原体（SPF）和 16 只常规饲养（CON）的豚鼠暴露于 0.4M 柠檬酸气溶胶中。采用 DCP 和 WBP 两种方法对它们进行咳嗽诱发试验。记录咳嗽次数和第一次咳嗽的潜伏期，并使用统计方法进行分析，以确定两种方法之间的显著差异：结果：WBP 导致的咳嗽次数明显更高（WBP 与 DCP 相比：SPF 为 13±9 对 2±3；CON 为 14±8 对 5±5；P 结论：方法差异对咳嗽反应有很大影响：方法差异对咳嗽反应有很大影响。WBP 为咳嗽评估提供了一种更可靠、更符合生理学原理的方法，这表明咳嗽研究需要标准化方案，以提高转化相关性。

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Advancing cough research: Methodological insights into cough challenge in guinea pig models using double chamber vs whole-body plethysmography

Objective

This study compares two methods of citric acid-induced cough in guinea pigs in whole-body plethysmography (WBP) and double chamber plethysmography (DCP) to evaluate their efficacy.

Methods

Sixteen specific pathogen-free (SPF) and sixteen conventionally-bred (CON) animals were exposed to 0.4 M citric acid aerosol. They underwent cough provocation using both DCP and WBP methods. The number of coughs and latency to the first cough were recorded and analysed using statistical methods to determine significant differences between the two techniques.

Results

WBP resulted in significantly higher cough counts (WBP vs. DCP: 13±9 vs 2±3 for SPF; 14±8 vs 5±5 for CON; p<0.0001) and shorter latency (WBP vs. DCP: 59±6 s vs 159±14 s for SPF; 77±4 s vs 112±12 s for CON; p<0.0001) compared to DCP in both groups.

Conclusion

Methodological differences substantially impact cough responses. WBP provides a more reliable and physiologically relevant methodology for cough assessment, suggesting the need for standardized protocols in cough research to enhance translational relevance.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.