构建柑橘叶枯病矮小相关病毒的感染性克隆并确认其致病性。

IF 4.4 2区 农林科学 Q1 PLANT SCIENCES
Jinfa Zhao, Song Zhang, Jiajun Wang, Binghai Lou, Yan Zhou
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引用次数: 0

摘要

柑橘矮化病(CCDD)严重影响柑橘产业。据推测,柑橘矮化病的病原体是柑橘矮化病相关病毒(CCDaV)。然而,这一推测并没有通过符合科赫假设得到证实。本研究在二元载体 pBinPLUS 中构建了由 1.6 倍串联的 CCDaV 基因组组成的感染性克隆,并通过真空渗透将其接种到尤里卡柠檬(Citrus limon)幼苗中。接种后 60 天,25% 的尤里卡柠檬幼苗出现皱缩和卷曲症状,与野生型病毒的症状相同。Western 印迹和嫁接传播试验证实,感染性克隆会系统感染尤里卡柠檬幼苗。此外,CCDaV 还能感染另外三个柑橘品种和一个杂交品种,但感染率不同。这些发现支持 CCDaV 是 CCDD 的主要病原体。感染性 CCDaV 克隆将有助于进一步研究病毒蛋白的功能以及 CCDaV 与宿主的分子相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Construction of an Infectious Clone of Citrus Chlorotic Dwarf-Associated Virus and Confirmation of Its Pathogenicity.

Citrus chlorotic dwarf disease (CCDD) seriously affects the citrus industry. Citrus chlorotic dwarf-associated virus (CCDaV) is speculated to be the causal agent of CCDD. However, this speculation has not been confirmed by fulfilling Koch's postulates. In this study, an infectious clone comprising a 1.6-fold tandem CCDaV genome in the binary vector pBinPLUS was constructed and agro-inoculated into 'Eureka' lemon (Citrus limon) seedlings through vacuum infiltration. At 60 days postinoculation, 25% of the 'Eureka' lemon seedlings developed symptoms of crinkling and curling that were the same as those associated with the wild-type virus. Western blotting and graft transmission assays confirmed that the infectious clone systemically infected 'Eureka' lemon seedlings. In addition, CCDaV can establish infection on three more Citrus species and one hybrid, although at different infection rates. These findings support that CCDaV is the primary causal agent of CCDD. The infectious CCDaV clone will allow further studies on the functions of viral proteins and molecular interactions of CCDaV with its hosts.

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来源期刊
Plant disease
Plant disease 农林科学-植物科学
CiteScore
5.10
自引率
13.30%
发文量
1993
审稿时长
2 months
期刊介绍: Plant Disease is the leading international journal for rapid reporting of research on new, emerging, and established plant diseases. The journal publishes papers that describe basic and applied research focusing on practical aspects of disease diagnosis, development, and management.
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