Briana M. Nosal , Staci N. Thornton , Manije Darooghegi Mofrad , Junichi R. Sakaki , Kyle J. Mahoney , Zachary Macdonald , Lauren Daddi , Thi Dong Binh Tran , George Weinstock , Yanjiao Zhou , Elaine Choung-Hee Lee , Ock K. Chun
{"title":"黑加仑能塑造肠道微生物群谱,并通过肠道-骨骼轴降低绝经后骨质疏松症的风险:一项试点随机对照试验提供的证据。","authors":"Briana M. Nosal , Staci N. Thornton , Manije Darooghegi Mofrad , Junichi R. Sakaki , Kyle J. Mahoney , Zachary Macdonald , Lauren Daddi , Thi Dong Binh Tran , George Weinstock , Yanjiao Zhou , Elaine Choung-Hee Lee , Ock K. Chun","doi":"10.1016/j.jnutbio.2024.109701","DOIUrl":null,"url":null,"abstract":"<div><p>This study aimed to investigate the effects of blackcurrant (BC) on gut microbiota abundance and composition, inflammatory and immune responses, and their relationship with bone mass changes. The effects of BC on bone mineral density (BMD), gut microbiota, and blood inflammatory and immune biomarkers were evaluated using DXA, stool and fasting blood collected from a pilot three-arm, randomized, double-blind, placebo-controlled clinical trial. Fifty-one peri- and early postmenopausal women aged 45–60 years were randomly assigned into one of three treatment groups for 6 months: control, low BC (392 mg/day) and high BC (784 mg/day); and 40 women completed the trial. BC supplementation for 6 months effectively mitigated the loss of whole-body BMD (<em>P</em><.05). Six-month changes (%) in peripheral IL-1β (<em>P</em>=.056) and RANKL (<em>P</em>=.052) for the high BC group were marginally significantly lower than the control group. Six-month changes in whole-body BMD were inversely correlated with changes in RANKL (<em>P</em><.01). In proteome analysis, four plasma proteins showed increased expression in the high BC group: IGFBP4, tetranectin, fetuin-B, and vitamin K-dependent protein S. BC dose-dependently increased the relative abundance of <em>Ruminococcus 2</em> (<em>P</em><.05), one of six bacteria correlated with BMD changes in the high BC group (<em>P</em><.05), suggesting it might be the key bacteria that drove bone protective effects. Daily BC consumption for 6 months mitigated bone loss in this population potentially through modulating the gut microbiota composition and suppressing osteoclastogenic cytokines. Larger-scale clinical trials on the potential benefits of BC and connection of <em>Ruminococcus 2</em> with BMD maintenance in postmenopausal women are warranted.</p><p>Trial Registration: NCT04431960, <span><span>https://classic.clinicaltrials.gov/ct2/show/NCT04431960</span><svg><path></path></svg></span>.</p></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"133 ","pages":"Article 109701"},"PeriodicalIF":4.8000,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Blackcurrants shape gut microbiota profile and reduce risk of postmenopausal osteoporosis via the gut-bone axis: Evidence from a pilot randomized controlled trial\",\"authors\":\"Briana M. Nosal , Staci N. Thornton , Manije Darooghegi Mofrad , Junichi R. Sakaki , Kyle J. 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BC supplementation for 6 months effectively mitigated the loss of whole-body BMD (<em>P</em><.05). Six-month changes (%) in peripheral IL-1β (<em>P</em>=.056) and RANKL (<em>P</em>=.052) for the high BC group were marginally significantly lower than the control group. Six-month changes in whole-body BMD were inversely correlated with changes in RANKL (<em>P</em><.01). In proteome analysis, four plasma proteins showed increased expression in the high BC group: IGFBP4, tetranectin, fetuin-B, and vitamin K-dependent protein S. BC dose-dependently increased the relative abundance of <em>Ruminococcus 2</em> (<em>P</em><.05), one of six bacteria correlated with BMD changes in the high BC group (<em>P</em><.05), suggesting it might be the key bacteria that drove bone protective effects. Daily BC consumption for 6 months mitigated bone loss in this population potentially through modulating the gut microbiota composition and suppressing osteoclastogenic cytokines. Larger-scale clinical trials on the potential benefits of BC and connection of <em>Ruminococcus 2</em> with BMD maintenance in postmenopausal women are warranted.</p><p>Trial Registration: NCT04431960, <span><span>https://classic.clinicaltrials.gov/ct2/show/NCT04431960</span><svg><path></path></svg></span>.</p></div>\",\"PeriodicalId\":16618,\"journal\":{\"name\":\"Journal of Nutritional Biochemistry\",\"volume\":\"133 \",\"pages\":\"Article 109701\"},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2024-07-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Nutritional Biochemistry\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0955286324001347\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nutritional Biochemistry","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0955286324001347","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
本研究旨在探讨黑加仑(BC)对肠道微生物群丰度和组成、炎症和免疫反应的影响,以及它们与骨质变化的关系。这项研究利用从一项三臂、随机、双盲、安慰剂对照临床试验中收集的 DXA、粪便和空腹血液,评估了黑加仑对骨矿物质密度(BMD)、肠道微生物群以及血液中炎症和免疫生物标志物的影响。51名年龄在45-60岁之间的围绝经期和绝经后早期妇女被随机分配到三个治疗组中的一个,为期6个月:对照组、低BC(392毫克/天)组和高BC(784毫克/天)组;40名妇女完成了试验。为期 6 个月的 BC 补充能有效缓解全身 BMD 的损失(P
Blackcurrants shape gut microbiota profile and reduce risk of postmenopausal osteoporosis via the gut-bone axis: Evidence from a pilot randomized controlled trial
This study aimed to investigate the effects of blackcurrant (BC) on gut microbiota abundance and composition, inflammatory and immune responses, and their relationship with bone mass changes. The effects of BC on bone mineral density (BMD), gut microbiota, and blood inflammatory and immune biomarkers were evaluated using DXA, stool and fasting blood collected from a pilot three-arm, randomized, double-blind, placebo-controlled clinical trial. Fifty-one peri- and early postmenopausal women aged 45–60 years were randomly assigned into one of three treatment groups for 6 months: control, low BC (392 mg/day) and high BC (784 mg/day); and 40 women completed the trial. BC supplementation for 6 months effectively mitigated the loss of whole-body BMD (P<.05). Six-month changes (%) in peripheral IL-1β (P=.056) and RANKL (P=.052) for the high BC group were marginally significantly lower than the control group. Six-month changes in whole-body BMD were inversely correlated with changes in RANKL (P<.01). In proteome analysis, four plasma proteins showed increased expression in the high BC group: IGFBP4, tetranectin, fetuin-B, and vitamin K-dependent protein S. BC dose-dependently increased the relative abundance of Ruminococcus 2 (P<.05), one of six bacteria correlated with BMD changes in the high BC group (P<.05), suggesting it might be the key bacteria that drove bone protective effects. Daily BC consumption for 6 months mitigated bone loss in this population potentially through modulating the gut microbiota composition and suppressing osteoclastogenic cytokines. Larger-scale clinical trials on the potential benefits of BC and connection of Ruminococcus 2 with BMD maintenance in postmenopausal women are warranted.
期刊介绍:
Devoted to advancements in nutritional sciences, The Journal of Nutritional Biochemistry presents experimental nutrition research as it relates to: biochemistry, molecular biology, toxicology, or physiology.
Rigorous reviews by an international editorial board of distinguished scientists ensure publication of the most current and key research being conducted in nutrition at the cellular, animal and human level. In addition to its monthly features of critical reviews and research articles, The Journal of Nutritional Biochemistry also periodically publishes emerging issues, experimental methods, and other types of articles.