开发的根瘤菌株提高了印度哈里亚纳邦豆科作物的土壤肥力和产量。

IF 3.5 4区 生物学 Q2 MICROBIOLOGY
Ikbal Shah, Khan M. Sarim, Virendra K. Sikka, Surjit S. Dudeja, Dharmender K. Gahlot
{"title":"开发的根瘤菌株提高了印度哈里亚纳邦豆科作物的土壤肥力和产量。","authors":"Ikbal Shah,&nbsp;Khan M. Sarim,&nbsp;Virendra K. Sikka,&nbsp;Surjit S. Dudeja,&nbsp;Dharmender K. Gahlot","doi":"10.1002/jobm.202400327","DOIUrl":null,"url":null,"abstract":"<p>Three strains of Gram-negative bacterium, <i>Rhizobium</i>, were developed by gamma (γ)-irradiation random mutagenesis. The developed strains were evaluated for their augmented features for symbiotic association, nitrogen fixation, and crop yield of three leguminous plants—chickpea, field-pea, and lentil—in agricultural fields of the northern Indian state of Haryana. Crops treated with developed mutants exhibited significant improvement in plant features and the yield of crops when compared to the control-uninoculated crops and crops grown with indigenous or commercial crop-specific strains of <i>Rhizobium</i>. This improvement was attributed to generated mutants, MbPrRz1 (on chickpea), MbPrRz2 (on lentil), and MbPrRz3 (on field-pea). Additionally, the cocultured symbiotic response of MbPrRz1 and MbPrRz2 mutants was found to be more pronounced on all three crops. The statistical analysis using Pearson's correlation coefficients revealed that nodulation and plant biomass were the most related parameters of crop yield. Among the effectiveness of developed mutants, MbPrRz1 yielded the best results for all three tested crops. Moreover, the developed mutants enhanced macro- and micronutrients of the experimental fields when compared with fields harboring the indigenous rhizobial community. These developed mutants were further genetically characterized, predominantly expressing nitrogen fixation marker, <i>nifH</i>, and appeared to belong to <i>Mesorhizobium ciceri</i> (MbPrRz1) and <i>Rhizobium leguminosarum</i> (both MbPrRz2 and MbPrRz3). In summary, this study highlights the potential of developed <i>Rhizobium</i> mutants as effective biofertilizers for sustainable agriculture, showcasing their ability to enhance symbiotic relationships, crop yield, and soil fertility.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.202400327","citationCount":"0","resultStr":"{\"title\":\"Developed Rhizobium Strains Enhance Soil Fertility and Yield of Legume Crops in Haryana, India\",\"authors\":\"Ikbal Shah,&nbsp;Khan M. Sarim,&nbsp;Virendra K. Sikka,&nbsp;Surjit S. Dudeja,&nbsp;Dharmender K. Gahlot\",\"doi\":\"10.1002/jobm.202400327\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Three strains of Gram-negative bacterium, <i>Rhizobium</i>, were developed by gamma (γ)-irradiation random mutagenesis. The developed strains were evaluated for their augmented features for symbiotic association, nitrogen fixation, and crop yield of three leguminous plants—chickpea, field-pea, and lentil—in agricultural fields of the northern Indian state of Haryana. Crops treated with developed mutants exhibited significant improvement in plant features and the yield of crops when compared to the control-uninoculated crops and crops grown with indigenous or commercial crop-specific strains of <i>Rhizobium</i>. This improvement was attributed to generated mutants, MbPrRz1 (on chickpea), MbPrRz2 (on lentil), and MbPrRz3 (on field-pea). Additionally, the cocultured symbiotic response of MbPrRz1 and MbPrRz2 mutants was found to be more pronounced on all three crops. The statistical analysis using Pearson's correlation coefficients revealed that nodulation and plant biomass were the most related parameters of crop yield. Among the effectiveness of developed mutants, MbPrRz1 yielded the best results for all three tested crops. Moreover, the developed mutants enhanced macro- and micronutrients of the experimental fields when compared with fields harboring the indigenous rhizobial community. These developed mutants were further genetically characterized, predominantly expressing nitrogen fixation marker, <i>nifH</i>, and appeared to belong to <i>Mesorhizobium ciceri</i> (MbPrRz1) and <i>Rhizobium leguminosarum</i> (both MbPrRz2 and MbPrRz3). In summary, this study highlights the potential of developed <i>Rhizobium</i> mutants as effective biofertilizers for sustainable agriculture, showcasing their ability to enhance symbiotic relationships, crop yield, and soil fertility.</p>\",\"PeriodicalId\":15101,\"journal\":{\"name\":\"Journal of Basic Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2024-07-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.202400327\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Basic Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jobm.202400327\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Basic Microbiology","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jobm.202400327","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

通过伽马(γ)辐照随机诱变,培育出了三株革兰氏阴性菌根瘤菌。在印度北部哈里亚纳邦的农田中,对所培育的菌株进行了评估,以确定其对三种豆科植物--豌豆、大田豌豆和小扁豆--的共生联合、固氮和作物产量的增强功能。与未接种根瘤菌的对照作物和使用本地或商业作物特异性根瘤菌株种植的作物相比,使用开发的突变体处理的作物在植物特征和作物产量方面都有显著改善。这种改善归功于所产生的突变体:MbPrRz1(鹰嘴豆)、MbPrRz2(扁豆)和 MbPrRz3(大田豌豆)。此外,还发现 MbPrRz1 和 MbPrRz2 突变体在三种作物上的共生反应更加明显。利用皮尔逊相关系数进行的统计分析显示,结瘤和植株生物量是与作物产量最相关的参数。在已开发突变体的有效性中,MbPrRz1 对所有三种受试作物的效果最好。此外,与本地根瘤菌群落相比,所开发的突变体提高了试验田的宏量和微量营养元素。对这些突变体进行了进一步的基因鉴定,发现它们主要表达固氮标记 nifH,似乎属于蝉膜中生孢子球菌(MbPrRz1)和豆根瘤菌(MbPrRz2 和 MbPrRz3)。总之,本研究强调了开发的根瘤菌突变体作为可持续农业的有效生物肥料的潜力,展示了它们增强共生关系、作物产量和土壤肥力的能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Developed Rhizobium Strains Enhance Soil Fertility and Yield of Legume Crops in Haryana, India

Three strains of Gram-negative bacterium, Rhizobium, were developed by gamma (γ)-irradiation random mutagenesis. The developed strains were evaluated for their augmented features for symbiotic association, nitrogen fixation, and crop yield of three leguminous plants—chickpea, field-pea, and lentil—in agricultural fields of the northern Indian state of Haryana. Crops treated with developed mutants exhibited significant improvement in plant features and the yield of crops when compared to the control-uninoculated crops and crops grown with indigenous or commercial crop-specific strains of Rhizobium. This improvement was attributed to generated mutants, MbPrRz1 (on chickpea), MbPrRz2 (on lentil), and MbPrRz3 (on field-pea). Additionally, the cocultured symbiotic response of MbPrRz1 and MbPrRz2 mutants was found to be more pronounced on all three crops. The statistical analysis using Pearson's correlation coefficients revealed that nodulation and plant biomass were the most related parameters of crop yield. Among the effectiveness of developed mutants, MbPrRz1 yielded the best results for all three tested crops. Moreover, the developed mutants enhanced macro- and micronutrients of the experimental fields when compared with fields harboring the indigenous rhizobial community. These developed mutants were further genetically characterized, predominantly expressing nitrogen fixation marker, nifH, and appeared to belong to Mesorhizobium ciceri (MbPrRz1) and Rhizobium leguminosarum (both MbPrRz2 and MbPrRz3). In summary, this study highlights the potential of developed Rhizobium mutants as effective biofertilizers for sustainable agriculture, showcasing their ability to enhance symbiotic relationships, crop yield, and soil fertility.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Basic Microbiology
Journal of Basic Microbiology 生物-微生物学
CiteScore
6.10
自引率
0.00%
发文量
134
审稿时长
1.8 months
期刊介绍: The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions. Papers published deal with: microbial interactions (pathogenic, mutualistic, environmental), ecology, physiology, genetics and cell biology/development, new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications) novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信