Ikbal Shah, Khan M. Sarim, Virendra K. Sikka, Surjit S. Dudeja, Dharmender K. Gahlot
{"title":"开发的根瘤菌株提高了印度哈里亚纳邦豆科作物的土壤肥力和产量。","authors":"Ikbal Shah, Khan M. Sarim, Virendra K. Sikka, Surjit S. Dudeja, Dharmender K. Gahlot","doi":"10.1002/jobm.202400327","DOIUrl":null,"url":null,"abstract":"<p>Three strains of Gram-negative bacterium, <i>Rhizobium</i>, were developed by gamma (γ)-irradiation random mutagenesis. The developed strains were evaluated for their augmented features for symbiotic association, nitrogen fixation, and crop yield of three leguminous plants—chickpea, field-pea, and lentil—in agricultural fields of the northern Indian state of Haryana. Crops treated with developed mutants exhibited significant improvement in plant features and the yield of crops when compared to the control-uninoculated crops and crops grown with indigenous or commercial crop-specific strains of <i>Rhizobium</i>. This improvement was attributed to generated mutants, MbPrRz1 (on chickpea), MbPrRz2 (on lentil), and MbPrRz3 (on field-pea). Additionally, the cocultured symbiotic response of MbPrRz1 and MbPrRz2 mutants was found to be more pronounced on all three crops. The statistical analysis using Pearson's correlation coefficients revealed that nodulation and plant biomass were the most related parameters of crop yield. Among the effectiveness of developed mutants, MbPrRz1 yielded the best results for all three tested crops. Moreover, the developed mutants enhanced macro- and micronutrients of the experimental fields when compared with fields harboring the indigenous rhizobial community. These developed mutants were further genetically characterized, predominantly expressing nitrogen fixation marker, <i>nifH</i>, and appeared to belong to <i>Mesorhizobium ciceri</i> (MbPrRz1) and <i>Rhizobium leguminosarum</i> (both MbPrRz2 and MbPrRz3). In summary, this study highlights the potential of developed <i>Rhizobium</i> mutants as effective biofertilizers for sustainable agriculture, showcasing their ability to enhance symbiotic relationships, crop yield, and soil fertility.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.202400327","citationCount":"0","resultStr":"{\"title\":\"Developed Rhizobium Strains Enhance Soil Fertility and Yield of Legume Crops in Haryana, India\",\"authors\":\"Ikbal Shah, Khan M. Sarim, Virendra K. Sikka, Surjit S. Dudeja, Dharmender K. Gahlot\",\"doi\":\"10.1002/jobm.202400327\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Three strains of Gram-negative bacterium, <i>Rhizobium</i>, were developed by gamma (γ)-irradiation random mutagenesis. The developed strains were evaluated for their augmented features for symbiotic association, nitrogen fixation, and crop yield of three leguminous plants—chickpea, field-pea, and lentil—in agricultural fields of the northern Indian state of Haryana. Crops treated with developed mutants exhibited significant improvement in plant features and the yield of crops when compared to the control-uninoculated crops and crops grown with indigenous or commercial crop-specific strains of <i>Rhizobium</i>. This improvement was attributed to generated mutants, MbPrRz1 (on chickpea), MbPrRz2 (on lentil), and MbPrRz3 (on field-pea). Additionally, the cocultured symbiotic response of MbPrRz1 and MbPrRz2 mutants was found to be more pronounced on all three crops. The statistical analysis using Pearson's correlation coefficients revealed that nodulation and plant biomass were the most related parameters of crop yield. Among the effectiveness of developed mutants, MbPrRz1 yielded the best results for all three tested crops. Moreover, the developed mutants enhanced macro- and micronutrients of the experimental fields when compared with fields harboring the indigenous rhizobial community. These developed mutants were further genetically characterized, predominantly expressing nitrogen fixation marker, <i>nifH</i>, and appeared to belong to <i>Mesorhizobium ciceri</i> (MbPrRz1) and <i>Rhizobium leguminosarum</i> (both MbPrRz2 and MbPrRz3). In summary, this study highlights the potential of developed <i>Rhizobium</i> mutants as effective biofertilizers for sustainable agriculture, showcasing their ability to enhance symbiotic relationships, crop yield, and soil fertility.</p>\",\"PeriodicalId\":15101,\"journal\":{\"name\":\"Journal of Basic Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2024-07-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.202400327\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Basic Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jobm.202400327\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Basic Microbiology","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jobm.202400327","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Developed Rhizobium Strains Enhance Soil Fertility and Yield of Legume Crops in Haryana, India
Three strains of Gram-negative bacterium, Rhizobium, were developed by gamma (γ)-irradiation random mutagenesis. The developed strains were evaluated for their augmented features for symbiotic association, nitrogen fixation, and crop yield of three leguminous plants—chickpea, field-pea, and lentil—in agricultural fields of the northern Indian state of Haryana. Crops treated with developed mutants exhibited significant improvement in plant features and the yield of crops when compared to the control-uninoculated crops and crops grown with indigenous or commercial crop-specific strains of Rhizobium. This improvement was attributed to generated mutants, MbPrRz1 (on chickpea), MbPrRz2 (on lentil), and MbPrRz3 (on field-pea). Additionally, the cocultured symbiotic response of MbPrRz1 and MbPrRz2 mutants was found to be more pronounced on all three crops. The statistical analysis using Pearson's correlation coefficients revealed that nodulation and plant biomass were the most related parameters of crop yield. Among the effectiveness of developed mutants, MbPrRz1 yielded the best results for all three tested crops. Moreover, the developed mutants enhanced macro- and micronutrients of the experimental fields when compared with fields harboring the indigenous rhizobial community. These developed mutants were further genetically characterized, predominantly expressing nitrogen fixation marker, nifH, and appeared to belong to Mesorhizobium ciceri (MbPrRz1) and Rhizobium leguminosarum (both MbPrRz2 and MbPrRz3). In summary, this study highlights the potential of developed Rhizobium mutants as effective biofertilizers for sustainable agriculture, showcasing their ability to enhance symbiotic relationships, crop yield, and soil fertility.
期刊介绍:
The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions.
Papers published deal with:
microbial interactions (pathogenic, mutualistic, environmental),
ecology,
physiology,
genetics and cell biology/development,
new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications)
novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).