{"title":"利用 B 扫描多焦点平均法抑制体内光学相干断层扫描测量中的多重散射。","authors":"Yiqiang Zhu, Lida Zhu, Yiheng Lim, Shuichi Makita, Yu Guo, Yoshiaki Yasuno","doi":"10.1364/BOE.524894","DOIUrl":null,"url":null,"abstract":"<p><p>We demonstrate a method that reduces the noise caused by multi-scattering (MS) photons in an <i>in vivo</i> optical coherence tomography image. This method combines a specially designed image acquisition (i.e., optical coherence tomography scan) scheme and subsequent complex signal processing. For the acquisition, multiple cross-sectional images (frames) are sequentially acquired while the depth position of the focus is altered for each frame by an electrically tunable lens. In the signal processing, the frames are numerically defocus-corrected, and complex averaged. Because of the inconsistency in the MS-photon trajectories among the different electrically tunable lens-induced defocus, this averaging reduces the MS signal. Unlike the previously demonstrated volume-wise multi-focus averaging method, our approach requires the sample to remain stable for only a brief period, approximately 70 ms, thus making it compatible with <i>in vivo</i> imaging. This method was validated using a scattering phantom and <i>in vivo</i> unanesthetized small fish samples, and was found to reduce MS noise even for unanesthetized <i>in vivo</i> measurement.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11249682/pdf/","citationCount":"0","resultStr":"{\"title\":\"Multiple scattering suppression for <i>in vivo</i> optical coherence tomography measurement using the B-scan-wise multi-focus averaging method.\",\"authors\":\"Yiqiang Zhu, Lida Zhu, Yiheng Lim, Shuichi Makita, Yu Guo, Yoshiaki Yasuno\",\"doi\":\"10.1364/BOE.524894\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We demonstrate a method that reduces the noise caused by multi-scattering (MS) photons in an <i>in vivo</i> optical coherence tomography image. This method combines a specially designed image acquisition (i.e., optical coherence tomography scan) scheme and subsequent complex signal processing. For the acquisition, multiple cross-sectional images (frames) are sequentially acquired while the depth position of the focus is altered for each frame by an electrically tunable lens. In the signal processing, the frames are numerically defocus-corrected, and complex averaged. Because of the inconsistency in the MS-photon trajectories among the different electrically tunable lens-induced defocus, this averaging reduces the MS signal. Unlike the previously demonstrated volume-wise multi-focus averaging method, our approach requires the sample to remain stable for only a brief period, approximately 70 ms, thus making it compatible with <i>in vivo</i> imaging. This method was validated using a scattering phantom and <i>in vivo</i> unanesthetized small fish samples, and was found to reduce MS noise even for unanesthetized <i>in vivo</i> measurement.</p>\",\"PeriodicalId\":8969,\"journal\":{\"name\":\"Biomedical optics express\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-06-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11249682/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical optics express\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1364/BOE.524894\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/7/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical optics express","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1364/BOE.524894","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
摘要
我们展示了一种可减少活体光学相干断层扫描图像中多重散射(MS)光子引起的噪声的方法。该方法结合了专门设计的图像采集(即光学相干断层扫描)方案和随后的复杂信号处理。在图像采集过程中,要依次采集多个横截面图像(帧),同时通过电动可调透镜改变每个帧的焦点深度位置。在信号处理过程中,对各帧图像进行散焦数值校正和复合平均。由于不同电调谐透镜引起的散焦在 MS 光子轨迹上存在不一致性,因此这种平均会降低 MS 信号。与之前展示的体积多焦点平均法不同,我们的方法只要求样品在大约 70 毫秒的短暂时间内保持稳定,因此与体内成像兼容。我们使用散射模型和未麻醉的活体小鱼样本对该方法进行了验证,发现即使在未麻醉的活体测量中,该方法也能降低 MS 噪音。
Multiple scattering suppression for in vivo optical coherence tomography measurement using the B-scan-wise multi-focus averaging method.
We demonstrate a method that reduces the noise caused by multi-scattering (MS) photons in an in vivo optical coherence tomography image. This method combines a specially designed image acquisition (i.e., optical coherence tomography scan) scheme and subsequent complex signal processing. For the acquisition, multiple cross-sectional images (frames) are sequentially acquired while the depth position of the focus is altered for each frame by an electrically tunable lens. In the signal processing, the frames are numerically defocus-corrected, and complex averaged. Because of the inconsistency in the MS-photon trajectories among the different electrically tunable lens-induced defocus, this averaging reduces the MS signal. Unlike the previously demonstrated volume-wise multi-focus averaging method, our approach requires the sample to remain stable for only a brief period, approximately 70 ms, thus making it compatible with in vivo imaging. This method was validated using a scattering phantom and in vivo unanesthetized small fish samples, and was found to reduce MS noise even for unanesthetized in vivo measurement.
期刊介绍:
The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including:
Tissue optics and spectroscopy
Novel microscopies
Optical coherence tomography
Diffuse and fluorescence tomography
Photoacoustic and multimodal imaging
Molecular imaging and therapies
Nanophotonic biosensing
Optical biophysics/photobiology
Microfluidic optical devices
Vision research.