开发和应用多糖测定法评估肠道渗透性。

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2024-01-01 Epub Date: 2024-07-18 DOI:10.1080/17576180.2024.2374168

Nicole A Schneck, Ahmed Moghieb, Claire Teague, Hermes Licea Perez

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引用次数: 0

摘要

目的：为了支持吲哚美辛挑战研究，对健康参与者的肠道渗透性进行评估，我们开发了生物分析测定法来测量人体尿液和血浆中的鼠李糖、赤藓糖醇、乳果糖和三氯蔗糖。方法：多糖检测采用 5μl 样品基质和简单的醋酸酐化学衍生，然后进行 RPLC-MS/MS 检测。结果尿液中鼠李糖和赤藓糖醇的定量范围为 1.00-1,000 微克/毫升，血浆中为 250,000 纳克/毫升。对于乳果糖和蔗糖素(三氯蔗糖)，生物测量的动态范围分别为 0.1-100 μg/ml（尿液）和 25-25,000 ng/ml（血浆）。结论这项工作有助于克服与单糖和双糖生物分析相关的一些常见分析难题，并提高了定量限。

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Development and application of a multi-sugar assay to assess intestinal permeability.

Aim: Bioanalytical assays to measure rhamnose, erythritol, lactulose and sucralose in human urine and plasma were developed to support an indomethacin challenge study for intestinal permeability assessment in healthy participants.Methods: The multi-sugar assays utilized 5-μl sample matrix and a simple chemical derivatization with acetic anhydride, followed by RPLC-MS/MS detection.Results: Rhamnose and erythritol quantification was established between 1.00-1,000 μg/ml in urine and 250-250,000 ng/ml in plasma. For lactulose and sucralose, dynamic ranges of 0.1-100 μg/ml (urine) and 25-25,000 ng/ml (plasma) were applied for biological measurements.Conclusion: This work helped overcome some of the common analytical challenges associated with the bioanalysis of mono- and disaccharides and achieved improved limits of quantification.

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.