开发和应用多糖测定法评估肠道渗透性。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-01-01 Epub Date: 2024-07-18 DOI:10.1080/17576180.2024.2374168

Nicole A Schneck, Ahmed Moghieb, Claire Teague, Hermes Licea Perez

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引用次数: 0

摘要

目的：为了支持吲哚美辛挑战研究，对健康参与者的肠道渗透性进行评估，我们开发了生物分析测定法来测量人体尿液和血浆中的鼠李糖、赤藓糖醇、乳果糖和三氯蔗糖。方法：多糖检测采用 5μl 样品基质和简单的醋酸酐化学衍生，然后进行 RPLC-MS/MS 检测。结果尿液中鼠李糖和赤藓糖醇的定量范围为 1.00-1,000 微克/毫升，血浆中为 250,000 纳克/毫升。对于乳果糖和蔗糖素(三氯蔗糖)，生物测量的动态范围分别为 0.1-100 μg/ml（尿液）和 25-25,000 ng/ml（血浆）。结论这项工作有助于克服与单糖和双糖生物分析相关的一些常见分析难题，并提高了定量限。

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Development and application of a multi-sugar assay to assess intestinal permeability.

Aim: Bioanalytical assays to measure rhamnose, erythritol, lactulose and sucralose in human urine and plasma were developed to support an indomethacin challenge study for intestinal permeability assessment in healthy participants.Methods: The multi-sugar assays utilized 5-μl sample matrix and a simple chemical derivatization with acetic anhydride, followed by RPLC-MS/MS detection.Results: Rhamnose and erythritol quantification was established between 1.00-1,000 μg/ml in urine and 250-250,000 ng/ml in plasma. For lactulose and sucralose, dynamic ranges of 0.1-100 μg/ml (urine) and 25-25,000 ng/ml (plasma) were applied for biological measurements.Conclusion: This work helped overcome some of the common analytical challenges associated with the bioanalysis of mono- and disaccharides and achieved improved limits of quantification.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.