Yuhan Chen, Nan Wang, Yuntai Lv, Chenyu Zhou, Qing Liang, Xingguang Su
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引用次数: 0
摘要
本研究利用双功能铜金属有机框架(Cu@MOF)纳米酶构建了一种新型、灵敏的碱性磷酸酶(ALP)荧光传感系统,该纳米酶具有优异的氧化酶模拟活性和荧光特性。由于 2-氨基-1,4-苯二甲酸(1,4-BDC-NH2)配体的存在,Cu@MOF 在 444 纳米波长处显示出优异的荧光性能。此外,Cu2+ 还赋予了 Cu@MOF 类似氧化酶的活性,它可以引发对苯二胺(PPD)被氧化成棕色产物(PPDox),并通过内滤效应(IFE)淬灭 Cu@MOF 的光致发光。由于 ATP 对 Cu2+ 具有优先亲和力,一旦加入 ATP,Cu@MOF 的催化活性就会明显降低,从而使 PPD 无法被氧化而恢复荧光。在 ALP 的存在下,ATP 被水解为腺苷和 Pi,这使得 Cu@MOF 重新获得催化活性,继续催化 PPDox 的生成。因此,Cu@MOF 的荧光再次减弱。ALP 活性与荧光强度的降低程度成正比。因此,这种新型荧光传感策略的线性范围为 0.5-60 U/L,检测限为 0.14 U/L。所建立的传感方法也可用于 ALP 抑制剂的筛选,并在测定人血清中的 ALP 活性水平方面取得了令人满意的结果。
Construction of the fluorescence sensing platform with a bifunctional Cu@MOF nanozyme for determination of alkaline phosphatase and its inhibitor.
In this work, a novel and sensitive fluorescence sensing system for alkaline phosphatase (ALP) was constructed using a bifunctional copper metal-organic framework (Cu@MOF) nanozyme, which had excellent oxidase-mimetic activity and fluorescence properties. Owing to the presence of 2-amino-1,4-benzenedicarboxylic acid (1,4-BDC-NH2) ligand, Cu@MOF displays excellent fluorescence performance at 444 nm. Additionally, Cu2+ endows the oxidase-like activity of Cu@MOF, which could trigger p-phenylenediamine (PPD) to be oxidized to a brown product (PPDox) and quench the photoluminescence of Cu@MOF through the inner filtration effect (IFE). As the preferential affinity of ATP for Cu2+, the catalytic activity of Cu@MOF was significantly reduced once ATP was added, thus PPD could not be oxidized and fluorescence was recovered. In the presence of ALP, ATP was hydrolyzed to adenosine and Pi, which allowed Cu@MOF to regain its catalytic activity and continued to catalyze the generation of PPDox. The fluorescence of Cu@MOF was therefore weakened once again. The ALP activity was directly proportional to the degree of decrease in fluorescence intensity. Thus, this novel fluorescence sensing strategy had a linear range of 0.5-60 U/L and the limit of detection was 0.14 U/L. The established sensing method could also be used to for ALP inhibitors screening, and achieved satisfactory results in determining the level of ALP activity in human serum.
期刊介绍:
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.