通过靶诱导过氧化物酶模拟氧化铁纳米颗粒修饰的金属有机框架的活性和荧光变化,建立 ATP 和铜离子的双通道传感平台

IF 8 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Yani Liu , Haoyu Chen , Lin Chai , Jing Liu , Shu Huang , Feng Liu , Mingjie Wei , Xiaohua Zhu , Youyu Zhang , Meiling Liu , Shouzhuo Yao
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引用次数: 0

摘要

同时或连续检测复杂样品中的多个目标物有多种应用,特别是在医疗诊断和环境监测方面。基于 PCN-224@Fe2O3 开发出了一种新型的双通道比色和荧光方法,用于连续检测三磷酸腺苷(ATP)和铜离子,PCN-224@Fe2O3 具有荧光和增强过氧化物酶样(POD)特性。PCN-224@Fe2O3 的合成涉及氧化铁在锆基金属有机框架(PCN-224)上的原位生长。通过催化无色化合物 3,3′,5,5′-四甲基联苯胺(TMB)在 H2O2 的存在下生成蓝色产物(oxTMB),PCN-224@Fe2O3 可以检测 ATP 和铜离子。其独特的机理是 ATP 通过与 PCN-224 中的 Zr 形成 Zr-O-P 键,削弱了 PCN-224@Fe2O3 的 POD 类活性,从而导致荧光恢复。铜离子通过与 ATP 发生螯合作用,抵消了 ATP 对 PCN-224@Fe2O3 的影响,从而在淬灭系统荧光的同时提高了 POD 样活性和 oxTMB 的含量。这有助于对 ATP 和 Cu2+ 进行比色 "开-关-开 "和荧光 "关-开-关 "开关传感。PCN-224@Fe2O3 在人血清中的 ATP 和 Cu2+ 检测中的应用取得了令人满意的结果。这一创新方法不仅为调节氧化物的模拟活性提供了一种手段,而且还为 ATP 和 Cu2+ 的检测提供了一种便捷、灵敏和准确的检测方法,极大地拓宽了 MOFs 和纳米酶在生物分析中的潜在应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A dual-channel sensing platform for ATP and copper ions via the target-induced peroxidase mimics activity and fluorescence variations of iron oxide nanoparticles-modified metal-organic frameworks

The simultaneous or continuous detection of multiple targets in complex samples is for various applications, notably in medical diagnostics and environmental monitoring. A novel dual-channel colorimetric and fluorescent method has been developed for the continuous detection of adenosine triphosphate (ATP) and copper ions based on PCN-224@Fe2O3, which exhibits both fluorescent and enhanced peroxidase-like (POD-like) properties. The synthesis of PCN-224@Fe2O3 involved the in-situ growth of ferric oxide on Zr-based metal-organic frameworks (PCN-224). Through catalyzing the colorless compound 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue product (oxTMB) in the presence of H2O2, PCN-224@Fe2O3 allows for the detection of ATP and copper ions. The unique mechanism involves ATP weakening the POD-like activity of PCN-224@Fe2O3 by forming Zr-O-P bonds with Zr in PCN-224, which also leads to fluorescence recovery. Copper ions, through chelating with ATP, counteract the effect of ATP on PCN-224@Fe2O3, resulting in increased POD-like activity and the amount of oxTMB alongside the quenching of the fluorescence of the system. This facilitates colorimetric “on-off-on” and fluorescence “off-on-off” switch sensing for ATP and Cu2+. The application of PCN-224@Fe2O3 in the detection of ATP and Cu2+ in human serum yielded satisfactory results. This innovative approach not only provides a means to regulate the mimic activity of oxides but also presents a convenient, sensitive, and accurate detection method for ATP and Cu2+, significantly broadening the potential application of MOFs and nanozymes in bioanalysis.

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来源期刊
Sensors and Actuators B: Chemical
Sensors and Actuators B: Chemical 工程技术-电化学
CiteScore
14.60
自引率
11.90%
发文量
1776
审稿时长
3.2 months
期刊介绍: Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.
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