Firdissa E Bokore, Kerry Boyle, Yuefeng Ruan, Curt A McCartney, Colin W Hiebert, Ron E Knox, Xiangyu Pei, Elsa Reimer, Karim Ammar, Wentao Zhang, Pierre Fobert, Richard D Cuthbert, Samia Berraies, Brent D McCallum
{"title":"绘制硬质小麦栽培品种 Strongfield 和其他 Triticum turgidum (L.) 品系的幼苗和成株叶锈病抗性基因图谱。","authors":"Firdissa E Bokore, Kerry Boyle, Yuefeng Ruan, Curt A McCartney, Colin W Hiebert, Ron E Knox, Xiangyu Pei, Elsa Reimer, Karim Ammar, Wentao Zhang, Pierre Fobert, Richard D Cuthbert, Samia Berraies, Brent D McCallum","doi":"10.1094/PHYTO-09-23-0348-R","DOIUrl":null,"url":null,"abstract":"<p><p>Durum wheat (<i>Triticum turgidum</i>) is threatened by the appearance of new virulent races of leaf rust, caused by <i>Puccinia triticina</i>, in recent years. This study was conducted to determine the leaf rust resistance in a modern Canadian durum cultivar, Strongfield. Six populations derived from crosses of Strongfield with six tetraploid wheat lines, respectively, were tested at the seedling plant stage with different <i>P. triticina</i> races. Two of the populations were evaluated for adult plant leaf rust infection in Canada and Mexico. A stepwise regression joint linkage quantitative trait locus (QTL) mapping and analysis by MapQTL were performed. Strongfield contributed the majority of QTLs detected, contributing seven QTLs detected in field tests and eight QTLs conditioning seedling resistance. A 1B QTL, <i>QLr-Spa-1B.1</i>, from Strongfield had a significant effect in both Canadian and Mexican field tests and corresponded with <i>Lr46</i>/<i>Yr29</i>. The remaining field QTLs were found in only the Canadian or the Mexican environment, not both. The QTL from Strongfield on 3A, <i>QLr-Spa-3A</i>, conferred seedling resistance to all races tested and had a significant effect in the field in Canada. This is the first report of <i>QLr-Spa-3A</i> and <i>Lr46</i>/<i>Yr29</i> as key components of genetic resistance in Canadian durum wheat. KASP markers were developed to detect <i>QLr-Spa-3A</i> for use in marker-assisted leaf rust resistance breeding. The susceptible parental lines contributed QTLs on 1A, 2B, and 5B that were effective in Mexican field tests and may be good targets to integrate into modern durum varieties to improve resistance to new durum virulent races.</p>","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2401-2411"},"PeriodicalIF":2.6000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mapping Seedling and Adult Plant Leaf Rust Resistance Genes in the Durum Wheat Cultivar Strongfield and Other <i>Triticum turgidum</i> Lines.\",\"authors\":\"Firdissa E Bokore, Kerry Boyle, Yuefeng Ruan, Curt A McCartney, Colin W Hiebert, Ron E Knox, Xiangyu Pei, Elsa Reimer, Karim Ammar, Wentao Zhang, Pierre Fobert, Richard D Cuthbert, Samia Berraies, Brent D McCallum\",\"doi\":\"10.1094/PHYTO-09-23-0348-R\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Durum wheat (<i>Triticum turgidum</i>) is threatened by the appearance of new virulent races of leaf rust, caused by <i>Puccinia triticina</i>, in recent years. This study was conducted to determine the leaf rust resistance in a modern Canadian durum cultivar, Strongfield. Six populations derived from crosses of Strongfield with six tetraploid wheat lines, respectively, were tested at the seedling plant stage with different <i>P. triticina</i> races. Two of the populations were evaluated for adult plant leaf rust infection in Canada and Mexico. A stepwise regression joint linkage quantitative trait locus (QTL) mapping and analysis by MapQTL were performed. Strongfield contributed the majority of QTLs detected, contributing seven QTLs detected in field tests and eight QTLs conditioning seedling resistance. A 1B QTL, <i>QLr-Spa-1B.1</i>, from Strongfield had a significant effect in both Canadian and Mexican field tests and corresponded with <i>Lr46</i>/<i>Yr29</i>. The remaining field QTLs were found in only the Canadian or the Mexican environment, not both. The QTL from Strongfield on 3A, <i>QLr-Spa-3A</i>, conferred seedling resistance to all races tested and had a significant effect in the field in Canada. This is the first report of <i>QLr-Spa-3A</i> and <i>Lr46</i>/<i>Yr29</i> as key components of genetic resistance in Canadian durum wheat. KASP markers were developed to detect <i>QLr-Spa-3A</i> for use in marker-assisted leaf rust resistance breeding. The susceptible parental lines contributed QTLs on 1A, 2B, and 5B that were effective in Mexican field tests and may be good targets to integrate into modern durum varieties to improve resistance to new durum virulent races.</p>\",\"PeriodicalId\":20410,\"journal\":{\"name\":\"Phytopathology\",\"volume\":\" \",\"pages\":\"2401-2411\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytopathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1094/PHYTO-09-23-0348-R\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/11/21 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PHYTO-09-23-0348-R","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/21 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Mapping Seedling and Adult Plant Leaf Rust Resistance Genes in the Durum Wheat Cultivar Strongfield and Other Triticum turgidum Lines.
Durum wheat (Triticum turgidum) is threatened by the appearance of new virulent races of leaf rust, caused by Puccinia triticina, in recent years. This study was conducted to determine the leaf rust resistance in a modern Canadian durum cultivar, Strongfield. Six populations derived from crosses of Strongfield with six tetraploid wheat lines, respectively, were tested at the seedling plant stage with different P. triticina races. Two of the populations were evaluated for adult plant leaf rust infection in Canada and Mexico. A stepwise regression joint linkage quantitative trait locus (QTL) mapping and analysis by MapQTL were performed. Strongfield contributed the majority of QTLs detected, contributing seven QTLs detected in field tests and eight QTLs conditioning seedling resistance. A 1B QTL, QLr-Spa-1B.1, from Strongfield had a significant effect in both Canadian and Mexican field tests and corresponded with Lr46/Yr29. The remaining field QTLs were found in only the Canadian or the Mexican environment, not both. The QTL from Strongfield on 3A, QLr-Spa-3A, conferred seedling resistance to all races tested and had a significant effect in the field in Canada. This is the first report of QLr-Spa-3A and Lr46/Yr29 as key components of genetic resistance in Canadian durum wheat. KASP markers were developed to detect QLr-Spa-3A for use in marker-assisted leaf rust resistance breeding. The susceptible parental lines contributed QTLs on 1A, 2B, and 5B that were effective in Mexican field tests and may be good targets to integrate into modern durum varieties to improve resistance to new durum virulent races.
期刊介绍：
Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.
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