Adel Al-Asfour, Maribasappa Karched, Syed Saad Bin Qasim, Gregor-Georg Zafiropoulos
{"title":"白色念珠菌在用于引导骨再生的聚四氟乙烯膜上的粘附。","authors":"Adel Al-Asfour, Maribasappa Karched, Syed Saad Bin Qasim, Gregor-Georg Zafiropoulos","doi":"10.1002/cre2.902","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objectives</h3>\n \n <p>Guided bone regeneration (GBR) is a core procedure used to regenerate bone defects. The aim of the study was to investigate the adherence of <i>Candida albicans</i> on six commercially available polytetrafluoroethylene (PTFE) membranes used in GBR procedures and the subsequent clinical consequences.</p>\n </section>\n \n <section>\n \n <h3> Materials and Methods</h3>\n \n <p>Six commercially available PTFE membranes were tested. Two of the membranes had a textured surface and the other four a plane, nontextured one. <i>C. albicans</i> (ATCC 24433) was cultured for 24 h, and its cell surface hydrophobicity was assessed using a modified method. <i>C. albicans</i> adhesion to membrane discs was studied by scanning electron microscopy (SEM) and real-time polymerase chain reaction (PCR).</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p><i>C. albicans</i> was found to be hydrophobic (77.25%). SEM analysis showed that <i>C. albicans</i> adherence to all membranes examined was characterized by patchy, scattered, and small clustered patterns except for one nontextured membrane with a most rough surface in which a thick biofilm was observed. Real-time PCR quantification revealed significantly greater adhesion of <i>C. albicans</i> cells to PTFE membranes than the control membrane (<i>p</i> ≤ .001) with the membranes having a textured surface exhibiting the highest count of 2680 × 10<sup>4</sup> cells/ml compared to the count of 707 × 10<sup>4</sup> cells/mL on those with a nontextured one (<i>p</i> ≤ .001). One membrane with nontextured surface, but with most rough surface was found to exhibit the highest count of 3010 × 10<sup>4</sup> cells/ml (<i>p</i> ≤ .05).</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>The results of this study indicate that <i>C. albicans</i> adhesion on membranes' surfaces depends on the degree of surface roughness and/or on the presence of a texture. Textured PTFE membranes and/or membranes high roughness showed significantly more adhered <i>C. albicans</i> cells. These findings can impact the surgeon's choice of GBR membrane and postoperative maintenance.</p>\n </section>\n </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"10 4","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.902","citationCount":"0","resultStr":"{\"title\":\"Adhesion of Candida albicans on PTFE membranes used in guided bone regeneration\",\"authors\":\"Adel Al-Asfour, Maribasappa Karched, Syed Saad Bin Qasim, Gregor-Georg Zafiropoulos\",\"doi\":\"10.1002/cre2.902\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Objectives</h3>\\n \\n <p>Guided bone regeneration (GBR) is a core procedure used to regenerate bone defects. The aim of the study was to investigate the adherence of <i>Candida albicans</i> on six commercially available polytetrafluoroethylene (PTFE) membranes used in GBR procedures and the subsequent clinical consequences.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Materials and Methods</h3>\\n \\n <p>Six commercially available PTFE membranes were tested. Two of the membranes had a textured surface and the other four a plane, nontextured one. <i>C. albicans</i> (ATCC 24433) was cultured for 24 h, and its cell surface hydrophobicity was assessed using a modified method. <i>C. albicans</i> adhesion to membrane discs was studied by scanning electron microscopy (SEM) and real-time polymerase chain reaction (PCR).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p><i>C. albicans</i> was found to be hydrophobic (77.25%). SEM analysis showed that <i>C. albicans</i> adherence to all membranes examined was characterized by patchy, scattered, and small clustered patterns except for one nontextured membrane with a most rough surface in which a thick biofilm was observed. Real-time PCR quantification revealed significantly greater adhesion of <i>C. albicans</i> cells to PTFE membranes than the control membrane (<i>p</i> ≤ .001) with the membranes having a textured surface exhibiting the highest count of 2680 × 10<sup>4</sup> cells/ml compared to the count of 707 × 10<sup>4</sup> cells/mL on those with a nontextured one (<i>p</i> ≤ .001). One membrane with nontextured surface, but with most rough surface was found to exhibit the highest count of 3010 × 10<sup>4</sup> cells/ml (<i>p</i> ≤ .05).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>The results of this study indicate that <i>C. albicans</i> adhesion on membranes' surfaces depends on the degree of surface roughness and/or on the presence of a texture. Textured PTFE membranes and/or membranes high roughness showed significantly more adhered <i>C. albicans</i> cells. 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Adhesion of Candida albicans on PTFE membranes used in guided bone regeneration
Objectives
Guided bone regeneration (GBR) is a core procedure used to regenerate bone defects. The aim of the study was to investigate the adherence of Candida albicans on six commercially available polytetrafluoroethylene (PTFE) membranes used in GBR procedures and the subsequent clinical consequences.
Materials and Methods
Six commercially available PTFE membranes were tested. Two of the membranes had a textured surface and the other four a plane, nontextured one. C. albicans (ATCC 24433) was cultured for 24 h, and its cell surface hydrophobicity was assessed using a modified method. C. albicans adhesion to membrane discs was studied by scanning electron microscopy (SEM) and real-time polymerase chain reaction (PCR).
Results
C. albicans was found to be hydrophobic (77.25%). SEM analysis showed that C. albicans adherence to all membranes examined was characterized by patchy, scattered, and small clustered patterns except for one nontextured membrane with a most rough surface in which a thick biofilm was observed. Real-time PCR quantification revealed significantly greater adhesion of C. albicans cells to PTFE membranes than the control membrane (p ≤ .001) with the membranes having a textured surface exhibiting the highest count of 2680 × 104 cells/ml compared to the count of 707 × 104 cells/mL on those with a nontextured one (p ≤ .001). One membrane with nontextured surface, but with most rough surface was found to exhibit the highest count of 3010 × 104 cells/ml (p ≤ .05).
Conclusion
The results of this study indicate that C. albicans adhesion on membranes' surfaces depends on the degree of surface roughness and/or on the presence of a texture. Textured PTFE membranes and/or membranes high roughness showed significantly more adhered C. albicans cells. These findings can impact the surgeon's choice of GBR membrane and postoperative maintenance.
期刊介绍：
Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.
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