{"title":"绿色合成的氧化铜纳米粒子可诱导胰腺癌细胞凋亡并上调 HOTAIR 和 HOTTIP。","authors":"Zahra Hosseini, Amirhossein Ahmadi, Ahmad Shadi, Seyed Javad Hosseini, Hossein Nikmanesh","doi":"10.1080/17435889.2024.2367958","DOIUrl":null,"url":null,"abstract":"<p><p><b>Aim:</b> Cu<sub>2</sub>O nanoparticles were synthesized using an extract from <i>S. latifolium</i> algae (SLCu<sub>2</sub>O NPs). Their effect on PANC-1 cells and the expression of two drug resistance-related lncRNAs were evaluated in comparison with Arsenic trioxide. <b>Materials & methods:</b> SLCu<sub>2</sub>O NPs were characterized using XRD, SEM, and TEM microscopies. The effects of SLCu<sub>2</sub>O NPs on cell cytotoxicity, cell cycle, and apoptosis, and expression of two drug resistance-related lncRNAs were examined using MTT assay, flow cytometry, and real-time PCR, respectively. <b>Results:</b> SLCu<sub>2</sub>O NPs demonstrated anti-cancer properties against PANC-1 cells comparable to Arsenic trioxide, and the expression of lncRNAs increased upon treatment with them. <b>Conclusion:</b> SLCu<sub>2</sub>O NPs demonstrate anti-cancer properties against PANC-1 cells; however, using gene silencing strategies along with SLCu<sub>2</sub>O NPs is suggested.</p>","PeriodicalId":74240,"journal":{"name":"Nanomedicine (London, England)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Green-synthesized copper oxide nanoparticles induce apoptosis and up-regulate <i>HOTAIR</i> and <i>HOTTIP</i> in pancreatic cancer cells.\",\"authors\":\"Zahra Hosseini, Amirhossein Ahmadi, Ahmad Shadi, Seyed Javad Hosseini, Hossein Nikmanesh\",\"doi\":\"10.1080/17435889.2024.2367958\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Aim:</b> Cu<sub>2</sub>O nanoparticles were synthesized using an extract from <i>S. latifolium</i> algae (SLCu<sub>2</sub>O NPs). Their effect on PANC-1 cells and the expression of two drug resistance-related lncRNAs were evaluated in comparison with Arsenic trioxide. <b>Materials & methods:</b> SLCu<sub>2</sub>O NPs were characterized using XRD, SEM, and TEM microscopies. The effects of SLCu<sub>2</sub>O NPs on cell cytotoxicity, cell cycle, and apoptosis, and expression of two drug resistance-related lncRNAs were examined using MTT assay, flow cytometry, and real-time PCR, respectively. <b>Results:</b> SLCu<sub>2</sub>O NPs demonstrated anti-cancer properties against PANC-1 cells comparable to Arsenic trioxide, and the expression of lncRNAs increased upon treatment with them. <b>Conclusion:</b> SLCu<sub>2</sub>O NPs demonstrate anti-cancer properties against PANC-1 cells; however, using gene silencing strategies along with SLCu<sub>2</sub>O NPs is suggested.</p>\",\"PeriodicalId\":74240,\"journal\":{\"name\":\"Nanomedicine (London, England)\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-07-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nanomedicine (London, England)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/17435889.2024.2367958\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanomedicine (London, England)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/17435889.2024.2367958","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Green-synthesized copper oxide nanoparticles induce apoptosis and up-regulate HOTAIR and HOTTIP in pancreatic cancer cells.
Aim: Cu2O nanoparticles were synthesized using an extract from S. latifolium algae (SLCu2O NPs). Their effect on PANC-1 cells and the expression of two drug resistance-related lncRNAs were evaluated in comparison with Arsenic trioxide. Materials & methods: SLCu2O NPs were characterized using XRD, SEM, and TEM microscopies. The effects of SLCu2O NPs on cell cytotoxicity, cell cycle, and apoptosis, and expression of two drug resistance-related lncRNAs were examined using MTT assay, flow cytometry, and real-time PCR, respectively. Results: SLCu2O NPs demonstrated anti-cancer properties against PANC-1 cells comparable to Arsenic trioxide, and the expression of lncRNAs increased upon treatment with them. Conclusion: SLCu2O NPs demonstrate anti-cancer properties against PANC-1 cells; however, using gene silencing strategies along with SLCu2O NPs is suggested.
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