NH4+ 在激活黄瓜(Cucumis sativus L.)缺铁反应中的信号功能。

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-07-15 DOI:10.1007/s00425-024-04480-5
Fatemeh Tavakoli, Roghieh Hajiboland, Dragana Bosnic, Predrag Bosnic, Miroslav Nikolic, Roser Tolra, Charlotte Poschenrieder
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引用次数: 0

摘要

主要结论NH4+ 是植物充分发挥基于还原的缺铁反应功能的必要条件。氮(N)在土壤中主要以硝酸盐(NO3-)或铵(NH4+)的形式存在。虽然平衡供应 NO3- 和 NH4+ 对植物最佳生长的重要性已被普遍接受,但其对铁(Fe)获取的重要性尚未得到充分研究。在这项研究中,水培黄瓜(Cucumis sativus L. cv. Maximus)植株在-铁条件下以 NO3- 作为唯一的氮源。当出现萎黄病时,植株根部或叶片补充 2 mM NH4Cl。NH4+ 处理增加了叶片 SPAD 和 HCl 可提取铁的浓度,同时降低了根凋亡体铁的浓度。根部一氧化氮浓度和 FRO 活性的增加以及乙烯作用抑制剂对 FRO 活性的抑制表明,NH4+ 处理的植物激活了策略 I 的成分。铵处理植物对稀溶的 Fe(OH)3 的利用率更高,根部释放的 H+、酚类和有机酸也更多。施用 NH4+ 增加了缺铁反应主调控因子(FIT)及其下游基因(AHA1、FRO2 和 IRT1)以及 EIN3 和 STOP1 的表达。通过时间分析和使用分根系统,我们认为浓度低于 2 mM 的 NH4+ 的永久存在足以产生未知信号,并导致铁缺乏相关基因的持续上调,从而增强铁获取机制。研究结果表明,NH4+ 似乎是植物基于还原作用的缺铁反应中一个广泛存在且以前未被重视的组成部分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Signaling function of NH<sub>4</sub><sup>+</sup> in the activation of Fe-deficiency response in cucumber (Cucumis sativus L.).

Signaling function of NH4+ in the activation of Fe-deficiency response in cucumber (Cucumis sativus L.).

Main conclusion: NH4+ is necessary for full functionality of reduction-based Fe deficiency response in plants. Nitrogen (N) is present in soil mainly as nitrate (NO3-) or ammonium (NH4+). Although the significance of a balanced supply of NO3- and NH4+ for optimal growth has been generally accepted, its importance for iron (Fe) acquisition has not been sufficiently investigated. In this work, hydroponically grown cucumber (Cucumis sativus L. cv. Maximus) plants were supplied with NO3- as the sole N source under -Fe conditions. Upon the appearance of chlorosis, plants were supplemented with 2 mM NH4Cl by roots or leaves. The NH4+ treatment increased leaf SPAD and the HCl-extractable Fe concentration while decreased root apoplastic Fe. A concomitant increase in the root concentration of nitric oxide and activity of FRO and its abolishment by an ethylene action inhibitor, indicated activation of the components of Strategy I in NH4+-treated plants. Ammonium-pretreated plants showed higher utilization capacity of sparingly soluble Fe(OH)3 and higher root release of H+, phenolics, and organic acids. The expression of the master regulator of Fe deficiency response (FIT) and its downstream genes (AHA1, FRO2, and IRT1) along with EIN3 and STOP1 was increased by NH4+ application. Temporal analyses and the employment of a split-root system enabled us to suggest that a permanent presence of NH4+ at concentrations lower than 2 mM is adequate to produce an unknown signal and causes a sustained upregulation of Fe deficiency-related genes, thus augmenting the Fe-acquisition machinery. The results indicate that NH4+ appears to be a widespread and previously underappreciated component of plant reduction-based Fe deficiency response.

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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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