锌基、洗必泰和精油漱口水的体外比较。

IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Thomas E Lallier, Brian S Goldfarb, Pooja Maney
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引用次数: 0

摘要

背景:牙周手术后经常会使用洗必泰(CHX)漱口水。最近上市的一种锌基漱口水品牌宣传其漱口水可替代洗必泰。本研究的目的是在体外评估该品牌锌漱口水对细胞存活、细胞运动和人牙龈成纤维细胞(HGFs)基因表达的影响。方法:将 HGFs 暴露于精油(EO)、CHX 和一种品牌的三种锌漱口水中,这些漱口水分别用于治疗口臭(ZnA)、口干(ZnB)和牙龈炎(ZnC)。每种漱口水都在一定浓度范围内测试了其对成纤维细胞生长因子存活和运动的影响。通过反转录聚合酶链反应(RT-PCR)评估细胞因子、白细胞介素和生长因子的基因表达,以此评估对炎症和伤口愈合的潜在影响:结果:CHX 和 ZnC 的细胞存活率在 10%稀释度时明显降低(p 结论:CHX 和 ZnC 的细胞毒性更强:与其他漱口水相比,ZnC 的细胞毒性更强,对细胞运动的抑制作用更大。因此,使用 ZnC 作为 CHX 的替代品可能会对牙周手术后的伤口愈合产生负面影响。白话摘要:与其他锌基漱口水相比,一种旨在替代洗必泰(通常在口腔手术后处方)的锌基漱口水显示出最大的口腔细胞死亡和细胞运动减少。这些锌漱口水还减少了参与调节炎症的蛋白质数量,从而可能减少对固定牙齿的骨骼的破坏。它们还改变了参与组织愈合的几种分子的数量。目前还不清楚这是否会加速或减缓口腔软组织的愈合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro comparison of zinc-based, chlorhexidine, and essential oil mouth rinses.

Background: Chlorhexidine (CHX)-based mouth rinses are frequently prescribed following periodontal surgeries. A more recently available brand of zinc-based mouth rinses advertises one of its mouth rinses as a substitute for chlorhexidine. The purpose of this study was to evaluate, in vitro, the effects of this brand of zinc-based mouth rinses on cell survival, cell motility, and gene expression of human gingival fibroblasts (HGFs).

Methods: HGFs were exposed to essential oil (EO), CHX, and three types of one brand of zinc-based mouth rinses designed to treat breath malodor (ZnA), dry mouth (ZnB), and gingivitis (ZnC). Each mouth rinse was tested over a range of concentrations for its effects on HGF survival and motility. Gene expression of cytokines, interleukins, and growth factors were evaluated via reverse transcriptase-polymerase chain reaction (RT-PCR), as a means to assess potential influences on inflammation and wound healing.

Results: Cell survival was significantly decreased for CHX and ZnC at 10% dilutions (p < 0.05). For all time points, cells exposed to ZnC displayed the greatest reduction in cell motility (p < 0.05). The various mouth rinses examined differentially altered the expression of growth factor transcripts. ZnC particularly enhanced the expression of BMP-2 and FGF-2.

Conclusion: ZnC was more cytotoxic and inhibited cell motility to a greater extent than any of the other mouth rinses. Therefore, using ZnC as an alternative to CHX could potentially have negative effects on wound healing after periodontal surgery. However, further investigation is required to confirm the clinical relevance of these in vitro findings.

Plain language summary: One type of zinc-based mouth rinse designed to replace chlorhexidine (often prescribed after oral surgeries) demonstrated the greatest oral cell death and reduction in cell movement when compared to other zinc-based mouth rinses. These zinc-based mouth rinses also reduced the amounts of proteins involved in regulating inflammation, potentially reducing the destruction of bone holding the teeth in place. They also changed the amounts of several molecules involved in tissue healing. It is unknown if this will speed or slow the healing of the soft tissues of the mouth.

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来源期刊
Journal of periodontology
Journal of periodontology 医学-牙科与口腔外科
CiteScore
9.10
自引率
7.00%
发文量
290
审稿时长
3-8 weeks
期刊介绍: The Journal of Periodontology publishes articles relevant to the science and practice of periodontics and related areas.
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