评估从土耳其公共建筑供水系统中分离出的嗜肺军团菌对大环内酯类药物的敏感性。

Northern clinics of Istanbul Pub Date : 2024-06-24 eCollection Date: 2024-01-01 DOI:10.14744/nci.2023.70104
Ahmet Aktas, Fatma Koksal Cakirlar
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引用次数: 0

摘要

目的:嗜肺军团菌(Lp)是一种需氧、无芽孢的革兰氏阴性细菌,在淡水栖息地(如河流和温泉)中无处不在,也可在人工水生环境中定植。Lp 能够在肺巨噬细胞内生长,这是形成感染的先决条件。因此,大环内酯类药物可在真核细胞内达到适当的治疗浓度,如阿奇霉素。本研究旨在调查 Lp 对大环内酯类药物的敏感性:方法:从伊斯坦布尔的公共建筑(医院和酒店)供水系统收集闪蒸前水样(n=143)。使用乳胶凝集试剂盒确认菌落为 Lp ST1、ST2-14 和非嗜肺菌 Lp:结果:使用乳胶凝集法在医院(23 个)和酒店(7 个)供水系统中检测到 30 种 Lp。无论血清型如何,排除未形成区域(≥256 mg/L)的菌株,阿奇霉素、红霉素和克拉霉素的主要 MIC 值分别为 0.61 mg/L(范围为 0.047-1 mg/L)、0.47 mg/L(范围为 0.047-1 mg/L)和 0.44 mg/L(范围为 0.047-1 mg/L)。大环内酯类药物的 MIC50 和 MIC90 值分别为:阿奇霉素 0.5 和 3 毫克/升;红霉素 0.38 和 1 毫克/升;克拉霉素 0.5 和 1 毫克/升。在不区分血清型的情况下,我们比较了菌株对所有抗菌药物的 MIC 值。我们没有发现抗生素 MIC 值之间存在明显差异(P=0.16):尽管我们的研究数据并不能完全反映断点,但仍需进一步开展流行病学研究,以统一 MIC 值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of macrolide susceptibility of Legionella pneumophila isolated from public buildings' water systems in Turkiye.

Objective: Legionella pneumophila (Lp) is aerobic, non-spore forming Gram-negative bacteria, which is ubiquitous in freshwater habitats, such as rivers and hot springs, as well as colonizing artificial aquatic environments. The ability of Lp to grow intracellularly within pulmonary macrophages is a prerequisite for the development of infection. Therefore, macrolides can achieve appropriate therapeutic concentrations in eukaryotic cells, such as azithromycin. This study aimed to investigate the macrolides susceptibility of Lp.

Methods: Pre-flash water samples (n=143) were collected from the public buildings (hospitals and hotels) water system in Istanbul. Colonies were confirmed as Lp ST1, ST2-14, and non-pneumophila Lp using latex agglutination kit.

Results: 30 Lp were detected in hospital (n=23) and hotel (n=7) water systems using latex agglutination. Regardless of serotype and excluding strains without zone formation (≥256 mg/L), the main MIC values of azithromycin, erythromycin and clarithromycin were 0.61 mg/L (range 0.047-1 mg/L), 0.47 mg/L (range 0.047-1 mg/L) and 0.44 mg/L (range 0.047-1 mg/L), respectively. The MIC50 and MIC90 values for macrolides were 0.5 and 3 mg/L for azithromycin, respectively; 0.38 and 1 mg/L for erythromycin, respectively; and 0.5 and 1 mg/L for clarithromycin, respectively. We compared the MIC values of the strains for all antimicrobial agents tested without serotype discrimination. We did not find a significant difference between the MIC values of the antibiotics (p=0.16).

Conclusion: Although the data obtained from our study do not fully reflect the breakpoints, further epidemiological studies are needed to standardize MIC values.

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