人体心脏多尺度三维解剖研究管道

IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Peter Hanna, Shumpei Mori, Takanori Sato, Shili Xu
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引用次数: 0

摘要

对因移植而被排斥的非衰竭性人类心脏的详细研究为进行微观和宏观尺度的结构分析提供了独特的机会。这些技术包括组织清除(通过改良免疫标记对溶剂清除器官进行三维成像)和免疫组化染色。中观检查程序包括立体解剖和微型计算机断层扫描(CT)。宏观检查程序包括大体解剖、摄影(包括anaglyphs和摄影测量)、CT和3D打印实物或虚拟解剖的心脏或整个心脏。在进行宏观检查前,可进行压力灌注固定,以保持心脏的三维结构和生理相关形态。将这些技术结合起来应用于人体心脏研究是独一无二的,对于了解心脏三维结构中不同解剖特征(如冠状血管和心肌神经支配)之间的关系至关重要。本规程详细描述了这些方法,并包含了具有代表性的结果,以说明人体心脏解剖学研究的进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pipeline for Multi-Scale Three-Dimensional Anatomic Study of the Human Heart.

Detailed study of non-failing human hearts rejected for transplantation provides a unique opportunity to perform structural analyses across microscopic and macroscopic scales. These techniques include tissue clearing (modified immunolabeling-enabled three-dimensional (3D) imaging of solvent-cleared organs) and immunohistochemical staining. Mesoscopic examination procedures include stereoscopic dissection and micro-computed tomographic (CT) scanning. Macroscopic examination procedures include gross dissection, photography (including anaglyphs and photogrammetry), CT, and 3D printing of the physically or virtually dissected or whole heart. Before macroscopic examination, pressure-perfusion fixation may be performed to maintain the 3D architecture and physiologically relevant morphology of the heart. The application of these techniques in combination to study the human heart is unique and crucial in understanding the relationship between distinct anatomic features such as coronary vasculature and myocardial innervation in the context of the 3D architecture of the heart. This protocol describes the methodologies in detail and includes representative results to illustrate progress in the research of human cardiac anatomy.

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来源期刊
Jove-Journal of Visualized Experiments
Jove-Journal of Visualized Experiments MULTIDISCIPLINARY SCIENCES-
CiteScore
2.10
自引率
0.00%
发文量
992
期刊介绍: JoVE, the Journal of Visualized Experiments, is the world''s first peer reviewed scientific video journal. Established in 2006, JoVE is devoted to publishing scientific research in a visual format to help researchers overcome two of the biggest challenges facing the scientific research community today; poor reproducibility and the time and labor intensive nature of learning new experimental techniques.
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