{"title":"[氟中毒小鼠髁突软骨自噬相关因子 LC3 和 p62 的损伤及表达变化]。","authors":"Ying Yu, Qing-Qing Weng, Yin-Yue Luo, Shu-Ran Yao, Fang-Yu Yi, Ying Zhang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>To study the damage and the expression of LC3 and p62 of condylar cartilage in fluorosis mouse.</p><p><strong>Methods: </strong>Thirty 4-week-old male C57BL/6 mice were randomly divided into control group and the experimental group with 15 animals in each group. The control group received regular drinking water and the experimental group received a fluoride concentration of 75 mg/L drinking water for 8 weeks. The structure of condylar cartilage was observed through modified safranine O-fast green FCF cartilage stain kit. Immunohistochemistry was used to detect the expression of MMP-13, type Ⅱ collagen and LC3 and p62. Two-way analysis of variance test was conducted for analysis of semi-quantitative results of immunohistochemistry using SPSS 22.0 software package.</p><p><strong>Results: </strong>Compared with the control group, the fibrocartilage layer of the experimental group became thinner, the condrocytes were smaller, and the staining became deeper.Immunohistochemistry results showed that the expression of MMP-13 and LC3 increased; the expression of type Ⅱ collagen and p62 decreased in the experimental group.</p><p><strong>Conclusions: </strong>There was degeneration of the condylar cartilage and autophagy in mice with drinking water containing 75 mg/L fluoride.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Damage and changes in expression of autophagy related factors LC3 and p62 of condylar cartilage in fluorosis mouse].\",\"authors\":\"Ying Yu, Qing-Qing Weng, Yin-Yue Luo, Shu-Ran Yao, Fang-Yu Yi, Ying Zhang\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>To study the damage and the expression of LC3 and p62 of condylar cartilage in fluorosis mouse.</p><p><strong>Methods: </strong>Thirty 4-week-old male C57BL/6 mice were randomly divided into control group and the experimental group with 15 animals in each group. The control group received regular drinking water and the experimental group received a fluoride concentration of 75 mg/L drinking water for 8 weeks. The structure of condylar cartilage was observed through modified safranine O-fast green FCF cartilage stain kit. Immunohistochemistry was used to detect the expression of MMP-13, type Ⅱ collagen and LC3 and p62. Two-way analysis of variance test was conducted for analysis of semi-quantitative results of immunohistochemistry using SPSS 22.0 software package.</p><p><strong>Results: </strong>Compared with the control group, the fibrocartilage layer of the experimental group became thinner, the condrocytes were smaller, and the staining became deeper.Immunohistochemistry results showed that the expression of MMP-13 and LC3 increased; the expression of type Ⅱ collagen and p62 decreased in the experimental group.</p><p><strong>Conclusions: </strong>There was degeneration of the condylar cartilage and autophagy in mice with drinking water containing 75 mg/L fluoride.</p>\",\"PeriodicalId\":21709,\"journal\":{\"name\":\"上海口腔医学\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"上海口腔医学\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"上海口腔医学","FirstCategoryId":"3","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
[Damage and changes in expression of autophagy related factors LC3 and p62 of condylar cartilage in fluorosis mouse].
Purpose: To study the damage and the expression of LC3 and p62 of condylar cartilage in fluorosis mouse.
Methods: Thirty 4-week-old male C57BL/6 mice were randomly divided into control group and the experimental group with 15 animals in each group. The control group received regular drinking water and the experimental group received a fluoride concentration of 75 mg/L drinking water for 8 weeks. The structure of condylar cartilage was observed through modified safranine O-fast green FCF cartilage stain kit. Immunohistochemistry was used to detect the expression of MMP-13, type Ⅱ collagen and LC3 and p62. Two-way analysis of variance test was conducted for analysis of semi-quantitative results of immunohistochemistry using SPSS 22.0 software package.
Results: Compared with the control group, the fibrocartilage layer of the experimental group became thinner, the condrocytes were smaller, and the staining became deeper.Immunohistochemistry results showed that the expression of MMP-13 and LC3 increased; the expression of type Ⅱ collagen and p62 decreased in the experimental group.
Conclusions: There was degeneration of the condylar cartilage and autophagy in mice with drinking water containing 75 mg/L fluoride.