在舌头和唾液微生物组中量化牙周炎相关的口腔菌群失调--综合数据分析。

IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Ren Jie Jacob Chew, Kai Soo Tan, Tsute Chen, Nezar Noor Al-Hebshi, Charlene Enhui Goh
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引用次数: 0

摘要

背景:牙周炎主要是由龈下生物膜菌群失调引起的。然而,牙周菌群失调的量化及其对其他口腔微生物壁龛的影响仍不清楚。本研究试图通过将临床相关的菌群失调指数应用于综合数据分析,量化牙周炎导致的舌头和唾液微生物组的菌群失调变化:方法:对美国国家生物技术信息中心(NCBI)数据库进行检索,以确定已发表的有关健康和牙周炎受试者唾液和舌头微生物组研究的生物项目。原始序列数据集采用标准化的生物信息学管道进行处理,并按其生态位和牙周状况进行分类。龈下微生物菌群失调指数(SMDI)是一种最初利用龈下微生物组开发的菌群失调指数,按种和属水平计算,并为每个生态位定制。利用接收器操作特征曲线评估了该指数对牙周炎的诊断准确性:结果:共纳入了四项研究,提供了 328 份微生物组样本。在物种和菌属水平上,牙周炎样本的 SMDI 都较高,但只有龈下生物膜和唾液的差异显著(p 结论):虽然对舌头微生物组的影响还需要进一步研究,但牙周炎相关的菌群失调会影响唾液微生物组,并可通过菌群失调指数进行量化。唾液微生物菌群失调作为一种评估牙周状况的方便的牙周生物标志物,具有潜在的公共卫生和临床应用诊断潜力。白话摘要:牙周炎是一种严重的牙龈炎症,会导致骨质流失,是一种由牙龈下好坏细菌失衡引起的疾病。然而,目前还不清楚牙龈中的这种细菌失衡如何影响口腔其他不同部位(如唾液和舌头)的细菌平衡。本研究使用了之前发表的四项研究的细菌数据集,共采集了 328 个细菌样本。使用统一的数据分析工作流程对数据进行了处理,并分别计算了牙龈下、唾液和舌头样本的细菌得分,即龈下微生物菌群失调指数(SMDI)。SMDI 能够区分每个口腔部位的健康和牙周炎,一般来说,牙周炎样本的得分更高,但这种差异仅在牙龈下和唾液中的细菌中显著。唾液得分也与牙龈下细菌一致相关。这项研究表明,与牙周炎相关的细菌失衡不仅存在于牙龈下的口腔部位,尤其是唾液中。因此,唾液细菌可用作评估牙龈疾病的便捷生物标志物,具有潜在的公共卫生和临床应用价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantifying periodontitis-associated oral dysbiosis in tongue and saliva microbiomes-An integrated data analysis.

Background: Periodontitis is primarily driven by subgingival biofilm dysbiosis. However, the quantification and impact of this periodontal dysbiosis on other oral microbial niches remain unclear. This study seeks to quantify the dysbiotic changes in tongue and salivary microbiomes resulting from periodontitis by applying a clinically relevant dysbiosis index to an integrated data analysis.

Methods: The National Center for Biotechnology Information (NCBI) database was searched to identify BioProjects with published studies on salivary and tongue microbiomes of healthy and periodontitis subjects. Raw sequence datasets were processed using a standardized bioinformatic pipeline and categorized by their ecological niche and periodontal status. The subgingival microbial dysbiosis index (SMDI), a dysbiosis index originally developed using the subgingival microbiome, was computed at species and genus levels and customized for each niche. Its diagnostic accuracy for periodontitis was evaluated using receiver operating characteristic curves.

Results: Four studies, contributing 328 microbiome samples, were included. At both species and genus levels, periodontitis samples had a higher SMDI, but the differences were only significant for subgingival biofilm and saliva (p < 0.001). However, SMDI showed good diagnostic accuracy for periodontitis status for all three niches (area under curve ranging from 0.76 to 0.90, p < 0.05). The dysbiosis index of subgingival biofilm was positively correlated with saliva consistently (p < 0.001) and with the tongue at the genus level (p = 0.036).

Conclusions: While the impact on the tongue microbiome requires further investigation, periodontitis-associated dysbiosis affects the salivary microbiome and is quantifiable using the dysbiosis index. The diagnostic potential of salivary microbial dysbiosis as a convenient periodontal biomarker for assessing periodontal status has potential public health and clinical applications.

Plain language summary: Periodontitis, a severe inflammation of the gums which causes bone loss, is a disease caused by an imbalance of good and bad bacteria under the gums. However, it is unclear how this bacterial imbalance in the gums affects the bacterial balance of other distinct parts of the mouth, such as the saliva and tongue. This study uses bacteria datasets of four previously published studies, contributing a total of 328 bacterial samples. The data were processed using a uniform data analysis workflow, and a bacterial score, the subgingival microbial dysbiosis index (SMDI), previously shown to capture periodontitis-associated bacteria imbalance, was calculated separately for samples from under the gums, the saliva, and the tongue. The SMDI was able to distinguish between health and periodontitis within each oral location, and in general, the scores were higher for periodontitis samples, though this difference was significant only for bacteria under the gums and in saliva. Saliva scores were also consistently correlated with bacteria under the gums. This study shows that periodontitis-associated bacterial imbalances are observed in oral locations beyond just under the gums, particularly the saliva. Thus, saliva bacteria may be used as a convenient biomarker for assessing gum disease, allowing for potential public health and clinical applications.

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来源期刊
Journal of periodontology
Journal of periodontology 医学-牙科与口腔外科
CiteScore
9.10
自引率
7.00%
发文量
290
审稿时长
3-8 weeks
期刊介绍: The Journal of Periodontology publishes articles relevant to the science and practice of periodontics and related areas.
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