Sérgio M. Morgado, Érica L. Fonseca, Ana Carolina P. Vicente
{"title":"携带 tet(X) 基因的伊丽莎白金色姬蛙的整合和共轭元素的流行与特征。","authors":"Sérgio M. Morgado, Érica L. Fonseca, Ana Carolina P. Vicente","doi":"10.1016/j.jgar.2024.07.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>To investigate the <em>tet</em>(X) gene, a determinant of tigecycline resistance, in the emerging pathogen <em>Elizabethkingia meningoseptica</em> and its association with an integrative and conjugative element (ICE).</p></div><div><h3>Methods</h3><p><em>All E. meningoseptica</em> genomes from the National Center for Biotechnology Information (<em>n</em> = 87) were retrieved and annotated for resistome searches using the CARD database. A phylogenic analysis was performed based on the <em>E. meningoseptica</em> core genome<em>.</em> The ICE was identified through comparative genomics with other ICEs occurring in <em>Elizabethkingia</em> spp.</p></div><div><h3>Results</h3><p>Phylogenetic analysis revealed <em>E. meningoseptica</em> genomes from six countries distributed across different lineages, some of which persisted for years. The common resistome of these genomes included <em>bla</em><sub>BlaB</sub>, <em>bla</em><sub>CME</sub>, <em>bla</em><sub>GOB</sub>, <em>ran</em>A/B, <em>aad</em>S, and <em>cat</em>B (genes associated with resistance to β-lactams, aminoglycosides, and chloramphenicol). Some genomes also presented additional resistance genes (<em>dfr</em>A, <em>ere</em>D, <em>bla</em><sub>VEB</sub>, <em>aad</em>S, and <em>tet</em>(X)). Interestingly, <em>tet</em>(X) and <em>aad</em>S were located in an ICE of 49 769 bp (ICEEmSQ101), which was fully obtained from the <em>E. meningoseptica</em> SQ101 genome. We also showed evidence that the other 27 genomes harboured this ICE. The distribution of ICEEmSQ101, carrying <em>tet</em>(X), was restricted to a single Chinese lineage.</p></div><div><h3>Conclusions</h3><p>The <em>tet</em>(X) gene is not prevalent in the species <em>E. meningoseptica</em>, as previously stated for the genus <em>Elizabethkingia</em>, since it is present only in a single Chinese lineage. We identified that several <em>E. meningoseptica</em> genomes harboured an ICE that mobilized the <em>Elizabethkingia tet</em>(X) gene and exhibited characteristics similar to the ICEs of other <em>Flavobacteria</em>, which would favour their transmission in this bacterial family.</p></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"38 ","pages":"Pages 227-230"},"PeriodicalIF":3.7000,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524001322/pdfft?md5=92abea5f89a5b94615fef94dba806c60&pid=1-s2.0-S2213716524001322-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Prevalence and characterization of an integrative and conjugative element carrying tet(X) gene in Elizabethkingia meningoseptica\",\"authors\":\"Sérgio M. Morgado, Érica L. Fonseca, Ana Carolina P. Vicente\",\"doi\":\"10.1016/j.jgar.2024.07.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><p>To investigate the <em>tet</em>(X) gene, a determinant of tigecycline resistance, in the emerging pathogen <em>Elizabethkingia meningoseptica</em> and its association with an integrative and conjugative element (ICE).</p></div><div><h3>Methods</h3><p><em>All E. meningoseptica</em> genomes from the National Center for Biotechnology Information (<em>n</em> = 87) were retrieved and annotated for resistome searches using the CARD database. A phylogenic analysis was performed based on the <em>E. meningoseptica</em> core genome<em>.</em> The ICE was identified through comparative genomics with other ICEs occurring in <em>Elizabethkingia</em> spp.</p></div><div><h3>Results</h3><p>Phylogenetic analysis revealed <em>E. meningoseptica</em> genomes from six countries distributed across different lineages, some of which persisted for years. The common resistome of these genomes included <em>bla</em><sub>BlaB</sub>, <em>bla</em><sub>CME</sub>, <em>bla</em><sub>GOB</sub>, <em>ran</em>A/B, <em>aad</em>S, and <em>cat</em>B (genes associated with resistance to β-lactams, aminoglycosides, and chloramphenicol). Some genomes also presented additional resistance genes (<em>dfr</em>A, <em>ere</em>D, <em>bla</em><sub>VEB</sub>, <em>aad</em>S, and <em>tet</em>(X)). Interestingly, <em>tet</em>(X) and <em>aad</em>S were located in an ICE of 49 769 bp (ICEEmSQ101), which was fully obtained from the <em>E. meningoseptica</em> SQ101 genome. We also showed evidence that the other 27 genomes harboured this ICE. The distribution of ICEEmSQ101, carrying <em>tet</em>(X), was restricted to a single Chinese lineage.</p></div><div><h3>Conclusions</h3><p>The <em>tet</em>(X) gene is not prevalent in the species <em>E. meningoseptica</em>, as previously stated for the genus <em>Elizabethkingia</em>, since it is present only in a single Chinese lineage. We identified that several <em>E. meningoseptica</em> genomes harboured an ICE that mobilized the <em>Elizabethkingia tet</em>(X) gene and exhibited characteristics similar to the ICEs of other <em>Flavobacteria</em>, which would favour their transmission in this bacterial family.</p></div>\",\"PeriodicalId\":15936,\"journal\":{\"name\":\"Journal of global antimicrobial resistance\",\"volume\":\"38 \",\"pages\":\"Pages 227-230\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-07-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2213716524001322/pdfft?md5=92abea5f89a5b94615fef94dba806c60&pid=1-s2.0-S2213716524001322-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of global antimicrobial resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213716524001322\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524001322","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Prevalence and characterization of an integrative and conjugative element carrying tet(X) gene in Elizabethkingia meningoseptica
Objectives
To investigate the tet(X) gene, a determinant of tigecycline resistance, in the emerging pathogen Elizabethkingia meningoseptica and its association with an integrative and conjugative element (ICE).
Methods
All E. meningoseptica genomes from the National Center for Biotechnology Information (n = 87) were retrieved and annotated for resistome searches using the CARD database. A phylogenic analysis was performed based on the E. meningoseptica core genome. The ICE was identified through comparative genomics with other ICEs occurring in Elizabethkingia spp.
Results
Phylogenetic analysis revealed E. meningoseptica genomes from six countries distributed across different lineages, some of which persisted for years. The common resistome of these genomes included blaBlaB, blaCME, blaGOB, ranA/B, aadS, and catB (genes associated with resistance to β-lactams, aminoglycosides, and chloramphenicol). Some genomes also presented additional resistance genes (dfrA, ereD, blaVEB, aadS, and tet(X)). Interestingly, tet(X) and aadS were located in an ICE of 49 769 bp (ICEEmSQ101), which was fully obtained from the E. meningoseptica SQ101 genome. We also showed evidence that the other 27 genomes harboured this ICE. The distribution of ICEEmSQ101, carrying tet(X), was restricted to a single Chinese lineage.
Conclusions
The tet(X) gene is not prevalent in the species E. meningoseptica, as previously stated for the genus Elizabethkingia, since it is present only in a single Chinese lineage. We identified that several E. meningoseptica genomes harboured an ICE that mobilized the Elizabethkingia tet(X) gene and exhibited characteristics similar to the ICEs of other Flavobacteria, which would favour their transmission in this bacterial family.
期刊介绍:
The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes.
JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR).
Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.