携带 tet(X) 基因的伊丽莎白金色姬蛙的整合和共轭元素的流行与特征。

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES
{"title":"携带 tet(X) 基因的伊丽莎白金色姬蛙的整合和共轭元素的流行与特征。","authors":"","doi":"10.1016/j.jgar.2024.07.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>To investigate the <em>tet</em>(X) gene, a determinant of tigecycline resistance, in the emerging pathogen <em>Elizabethkingia meningoseptica</em> and its association with an integrative and conjugative element (ICE).</p></div><div><h3>Methods</h3><p><em>All E. meningoseptica</em> genomes from the National Center for Biotechnology Information (<em>n</em> = 87) were retrieved and annotated for resistome searches using the CARD database. A phylogenic analysis was performed based on the <em>E. meningoseptica</em> core genome<em>.</em> The ICE was identified through comparative genomics with other ICEs occurring in <em>Elizabethkingia</em> spp.</p></div><div><h3>Results</h3><p>Phylogenetic analysis revealed <em>E. meningoseptica</em> genomes from six countries distributed across different lineages, some of which persisted for years. The common resistome of these genomes included <em>bla</em><sub>BlaB</sub>, <em>bla</em><sub>CME</sub>, <em>bla</em><sub>GOB</sub>, <em>ran</em>A/B, <em>aad</em>S, and <em>cat</em>B (genes associated with resistance to β-lactams, aminoglycosides, and chloramphenicol). Some genomes also presented additional resistance genes (<em>dfr</em>A, <em>ere</em>D, <em>bla</em><sub>VEB</sub>, <em>aad</em>S, and <em>tet</em>(X)). Interestingly, <em>tet</em>(X) and <em>aad</em>S were located in an ICE of 49 769 bp (ICEEmSQ101), which was fully obtained from the <em>E. meningoseptica</em> SQ101 genome. We also showed evidence that the other 27 genomes harboured this ICE. The distribution of ICEEmSQ101, carrying <em>tet</em>(X), was restricted to a single Chinese lineage.</p></div><div><h3>Conclusions</h3><p>The <em>tet</em>(X) gene is not prevalent in the species <em>E. meningoseptica</em>, as previously stated for the genus <em>Elizabethkingia</em>, since it is present only in a single Chinese lineage. We identified that several <em>E. meningoseptica</em> genomes harboured an ICE that mobilized the <em>Elizabethkingia tet</em>(X) gene and exhibited characteristics similar to the ICEs of other <em>Flavobacteria</em>, which would favour their transmission in this bacterial family.</p></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524001322/pdfft?md5=92abea5f89a5b94615fef94dba806c60&pid=1-s2.0-S2213716524001322-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Prevalence and characterization of an integrative and conjugative element carrying tet(X) gene in Elizabethkingia meningoseptica\",\"authors\":\"\",\"doi\":\"10.1016/j.jgar.2024.07.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><p>To investigate the <em>tet</em>(X) gene, a determinant of tigecycline resistance, in the emerging pathogen <em>Elizabethkingia meningoseptica</em> and its association with an integrative and conjugative element (ICE).</p></div><div><h3>Methods</h3><p><em>All E. meningoseptica</em> genomes from the National Center for Biotechnology Information (<em>n</em> = 87) were retrieved and annotated for resistome searches using the CARD database. A phylogenic analysis was performed based on the <em>E. meningoseptica</em> core genome<em>.</em> The ICE was identified through comparative genomics with other ICEs occurring in <em>Elizabethkingia</em> spp.</p></div><div><h3>Results</h3><p>Phylogenetic analysis revealed <em>E. meningoseptica</em> genomes from six countries distributed across different lineages, some of which persisted for years. The common resistome of these genomes included <em>bla</em><sub>BlaB</sub>, <em>bla</em><sub>CME</sub>, <em>bla</em><sub>GOB</sub>, <em>ran</em>A/B, <em>aad</em>S, and <em>cat</em>B (genes associated with resistance to β-lactams, aminoglycosides, and chloramphenicol). Some genomes also presented additional resistance genes (<em>dfr</em>A, <em>ere</em>D, <em>bla</em><sub>VEB</sub>, <em>aad</em>S, and <em>tet</em>(X)). Interestingly, <em>tet</em>(X) and <em>aad</em>S were located in an ICE of 49 769 bp (ICEEmSQ101), which was fully obtained from the <em>E. meningoseptica</em> SQ101 genome. We also showed evidence that the other 27 genomes harboured this ICE. The distribution of ICEEmSQ101, carrying <em>tet</em>(X), was restricted to a single Chinese lineage.</p></div><div><h3>Conclusions</h3><p>The <em>tet</em>(X) gene is not prevalent in the species <em>E. meningoseptica</em>, as previously stated for the genus <em>Elizabethkingia</em>, since it is present only in a single Chinese lineage. We identified that several <em>E. meningoseptica</em> genomes harboured an ICE that mobilized the <em>Elizabethkingia tet</em>(X) gene and exhibited characteristics similar to the ICEs of other <em>Flavobacteria</em>, which would favour their transmission in this bacterial family.</p></div>\",\"PeriodicalId\":15936,\"journal\":{\"name\":\"Journal of global antimicrobial resistance\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-07-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2213716524001322/pdfft?md5=92abea5f89a5b94615fef94dba806c60&pid=1-s2.0-S2213716524001322-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of global antimicrobial resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213716524001322\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524001322","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

摘要

目的:研究新出现的病原体伊丽莎白金格脑膜炎菌的 tet(X)基因及其与 ICE 的关系:研究新出现的病原体伊丽莎白金色葡萄球菌(Elizabethkingia meningoseptica)中决定替加环素抗性的 tet(X) 基因及其与 ICE 的关联:方法:利用 CARD 数据库检索并注释了美国国家科学与生物研究所(NCBI)的所有脑膜金线虫基因组(n=87),用于抗性组搜索,并根据脑膜金线虫核心基因组进行了系统发育分析。通过与伊丽莎白金线虫属(Elizabethkingia spp.)中出现的 ICE 进行比较基因组学鉴定:系统发生分析表明,来自六个国家的脑膜脑炎病毒基因组分布在不同的系谱中,其中一些系谱已存在多年。这些基因组的共同抗性基因组包括 blaBlaB、blaCME、blaGOB、ranA/B、aadS 和 catB(与对 β-内酰胺类、氨基糖苷类和氯霉素的抗性有关的基因)。一些基因组还出现了额外的抗性基因(dfrA、ereD、blaVEB、aadS 和 tet(X))。有趣的是,tet(X)和 aadS 基因位于一个 49 769 bp 的 ICE(ICEEmSQ101)中,该 ICE 完全来自脑膜塞菌 SQ101 基因组。我们还提出了其他 27 个基因组也存在该 ICE 的证据。携带tet(X)基因的ICEEmSQ101的分布仅限于一个中国品系:结论:tet(X)基因在脑膜脑炎伊丽莎白金线虫中并不普遍,这与之前伊丽莎白金线虫属的情况不同,因为它基本上只存在于一个中国品系中。我们发现,脑膜色素梭菌的几个基因组中都含有能调动 tet(X)基因的 ICE,并表现出与其他黄杆菌的 ICE 相似的特征,这有利于它们在该细菌家族中的传播。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Prevalence and characterization of an integrative and conjugative element carrying tet(X) gene in Elizabethkingia meningoseptica

Objectives

To investigate the tet(X) gene, a determinant of tigecycline resistance, in the emerging pathogen Elizabethkingia meningoseptica and its association with an integrative and conjugative element (ICE).

Methods

All E. meningoseptica genomes from the National Center for Biotechnology Information (n = 87) were retrieved and annotated for resistome searches using the CARD database. A phylogenic analysis was performed based on the E. meningoseptica core genome. The ICE was identified through comparative genomics with other ICEs occurring in Elizabethkingia spp.

Results

Phylogenetic analysis revealed E. meningoseptica genomes from six countries distributed across different lineages, some of which persisted for years. The common resistome of these genomes included blaBlaB, blaCME, blaGOB, ranA/B, aadS, and catB (genes associated with resistance to β-lactams, aminoglycosides, and chloramphenicol). Some genomes also presented additional resistance genes (dfrA, ereD, blaVEB, aadS, and tet(X)). Interestingly, tet(X) and aadS were located in an ICE of 49 769 bp (ICEEmSQ101), which was fully obtained from the E. meningoseptica SQ101 genome. We also showed evidence that the other 27 genomes harboured this ICE. The distribution of ICEEmSQ101, carrying tet(X), was restricted to a single Chinese lineage.

Conclusions

The tet(X) gene is not prevalent in the species E. meningoseptica, as previously stated for the genus Elizabethkingia, since it is present only in a single Chinese lineage. We identified that several E. meningoseptica genomes harboured an ICE that mobilized the Elizabethkingia tet(X) gene and exhibited characteristics similar to the ICEs of other Flavobacteria, which would favour their transmission in this bacterial family.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信