柬埔寨产ESBL和碳青霉烯酶大肠埃希菌和肺炎克雷伯氏菌临床分离菌株对可乐定的耐药性。

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES
Mallorie Hide , Soda Meng , Sokleaph Cheng , Anne-Laure Bañuls , Santy KY , Chantana YAY , Denis Laurent , Gauthier Delvallez
{"title":"柬埔寨产ESBL和碳青霉烯酶大肠埃希菌和肺炎克雷伯氏菌临床分离菌株对可乐定的耐药性。","authors":"Mallorie Hide ,&nbsp;Soda Meng ,&nbsp;Sokleaph Cheng ,&nbsp;Anne-Laure Bañuls ,&nbsp;Santy KY ,&nbsp;Chantana YAY ,&nbsp;Denis Laurent ,&nbsp;Gauthier Delvallez","doi":"10.1016/j.jgar.2024.06.017","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>Despite the critical importance of colistin as a last-resort antibiotic, limited studies have investigated colistin resistance in human infections in Cambodia. This study aimed to investigate the colistin resistance and its molecular determinants among Extended-spectrum beta-lactamase (ESBL)- and carbapenemase-producing (CP) <em>Klebsiella pneumoniae</em> (<em>K. pneumoniae</em>) and <em>Escherichia coli</em> (<em>E. coli</em>) isolated in Cambodia between 2016 and 2020.</p></div><div><h3>Methods</h3><p><em>E. coli</em> (<em>n</em> = 223) and <em>K. pneumoniae</em> (<em>n</em> = 39) were tested for colistin minimum inhibitory concentration (MIC) by broth microdilution. Resistant isolates were subjected to polymerase chain reaction (PCR) for detection of mobile colistin resistance genes (<em>mcr</em>) and chromosomal mutations in the two-component system (TCS).</p></div><div><h3>Results</h3><p>Eighteen isolates (10 <em>K. pneumoniae</em> and 8 <em>E. coli</em>) revealed colistin resistance with a rate of 5.9% in <em>E. coli</em> and 34.8% in <em>K. pneumoniae</em> among ESBL isolates, and 1% in <em>E. coli</em> and 12.5% in <em>K. pneumoniae</em> among CP isolates. The resistance was associated with <em>mcr</em> variants (13/18 isolates, <em>mcr-1, mcr</em>-<em>3</em>, and <em>mcr-8.2</em>) and TCS mutations within <em>E. coli</em> and <em>K. pneumoniae</em>, with the first detection of <em>mcr-8.2</em> in Cambodia, the discovery of new mutations potentially associated to colistin resistance in the TCS of <em>E. coli</em> (PhoP I47V, PhoQ N352K, PmrB G19R, and PmrD G85R) and the co-occurrence of <em>mcr</em> genes and colistin resistance conferring TCS mutations in 11 of 18 isolates.</p></div><div><h3>Conclusions</h3><p>The findings highlight the presence of colistin resistance in ESBL- and CP- <em>Enterobacteriaceae</em> involved in human infections in Cambodia as well as chromosomal mutations in TCS and the emergence of <em>mcr</em>-<em>8.2</em> in <em>E. coli</em> and <em>K. pneumoniae</em>. It underscores the need for continuous surveillance, antimicrobial stewardship, and control measures to mitigate the spread of colistin resistance.</p></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"38 ","pages":"Pages 236-244"},"PeriodicalIF":3.7000,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524001346/pdfft?md5=baecadd2c501f3b5b9504df2e7990299&pid=1-s2.0-S2213716524001346-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Colistin resistance in ESBL- and Carbapenemase-producing Escherichia coli and Klebsiella pneumoniae clinical isolates in Cambodia\",\"authors\":\"Mallorie Hide ,&nbsp;Soda Meng ,&nbsp;Sokleaph Cheng ,&nbsp;Anne-Laure Bañuls ,&nbsp;Santy KY ,&nbsp;Chantana YAY ,&nbsp;Denis Laurent ,&nbsp;Gauthier Delvallez\",\"doi\":\"10.1016/j.jgar.2024.06.017\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><p>Despite the critical importance of colistin as a last-resort antibiotic, limited studies have investigated colistin resistance in human infections in Cambodia. This study aimed to investigate the colistin resistance and its molecular determinants among Extended-spectrum beta-lactamase (ESBL)- and carbapenemase-producing (CP) <em>Klebsiella pneumoniae</em> (<em>K. pneumoniae</em>) and <em>Escherichia coli</em> (<em>E. coli</em>) isolated in Cambodia between 2016 and 2020.</p></div><div><h3>Methods</h3><p><em>E. coli</em> (<em>n</em> = 223) and <em>K. pneumoniae</em> (<em>n</em> = 39) were tested for colistin minimum inhibitory concentration (MIC) by broth microdilution. Resistant isolates were subjected to polymerase chain reaction (PCR) for detection of mobile colistin resistance genes (<em>mcr</em>) and chromosomal mutations in the two-component system (TCS).</p></div><div><h3>Results</h3><p>Eighteen isolates (10 <em>K. pneumoniae</em> and 8 <em>E. coli</em>) revealed colistin resistance with a rate of 5.9% in <em>E. coli</em> and 34.8% in <em>K. pneumoniae</em> among ESBL isolates, and 1% in <em>E. coli</em> and 12.5% in <em>K. pneumoniae</em> among CP isolates. The resistance was associated with <em>mcr</em> variants (13/18 isolates, <em>mcr-1, mcr</em>-<em>3</em>, and <em>mcr-8.2</em>) and TCS mutations within <em>E. coli</em> and <em>K. pneumoniae</em>, with the first detection of <em>mcr-8.2</em> in Cambodia, the discovery of new mutations potentially associated to colistin resistance in the TCS of <em>E. coli</em> (PhoP I47V, PhoQ N352K, PmrB G19R, and PmrD G85R) and the co-occurrence of <em>mcr</em> genes and colistin resistance conferring TCS mutations in 11 of 18 isolates.</p></div><div><h3>Conclusions</h3><p>The findings highlight the presence of colistin resistance in ESBL- and CP- <em>Enterobacteriaceae</em> involved in human infections in Cambodia as well as chromosomal mutations in TCS and the emergence of <em>mcr</em>-<em>8.2</em> in <em>E. coli</em> and <em>K. pneumoniae</em>. It underscores the need for continuous surveillance, antimicrobial stewardship, and control measures to mitigate the spread of colistin resistance.</p></div>\",\"PeriodicalId\":15936,\"journal\":{\"name\":\"Journal of global antimicrobial resistance\",\"volume\":\"38 \",\"pages\":\"Pages 236-244\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-07-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2213716524001346/pdfft?md5=baecadd2c501f3b5b9504df2e7990299&pid=1-s2.0-S2213716524001346-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of global antimicrobial resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213716524001346\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524001346","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

摘要

目的: 。尽管可乐定作为最后的抗生素至关重要,但对柬埔寨人类感染中可乐定耐药性的研究却十分有限。本研究旨在调查 2016 年至 2020 年期间在柬埔寨分离的广谱β-内酰胺酶(ESBL)和产碳青霉烯酶(CP)肺炎克雷伯菌(KP)和大肠埃希菌(EC)对可乐定的耐药性及其分子决定因素。通过肉汤微量稀释法检测大肠埃希菌(n=223)和肺炎克雷伯菌(n=39)的可乐定最低抑菌浓度(MIC)。对耐药分离物进行聚合酶链式反应(PCR),检测移动的可乐定耐药基因(mcr)和双组分系统(TCS)的染色体突变。18个分离株(10个KP,8个EC)显示出对可乐定的耐药性,ESBL分离株中,EC株的耐药率为5.9%,KP株的耐药率为34.8%;CP分离株中,EC株的耐药率为1%,KP株的耐药率为12.5%。耐药性与 mcr 变异(13/18 个分离株,mcr-1、mcr-3 和 mcr-8.2)以及欧共体和金边省的 TCS 变异有关,其中 mcr-8.2 是首次在柬埔寨发现。在柬埔寨首次发现了 mcr-8.2,在 EC 的 TCS 中发现了可能与耐大肠菌素有关的新突变(PhoP I47V、PhoQ N352K、PmrB G19R、PmrD G85R),在 11/18 个分离株中同时发现了 mcr 基因和耐大肠菌素的 TCS 突变:研究结果表明,柬埔寨涉及人类感染的 ESBL- 和 CP- 肠杆菌科细菌中存在对可乐定的耐药性、TCS 染色体突变以及在 EC 和 KP 中出现 mcr-8.2。这凸显了持续监测、抗菌药物管理和控制措施的必要性,以减少可乐定耐药性的传播。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Colistin resistance in ESBL- and Carbapenemase-producing Escherichia coli and Klebsiella pneumoniae clinical isolates in Cambodia

Objectives

Despite the critical importance of colistin as a last-resort antibiotic, limited studies have investigated colistin resistance in human infections in Cambodia. This study aimed to investigate the colistin resistance and its molecular determinants among Extended-spectrum beta-lactamase (ESBL)- and carbapenemase-producing (CP) Klebsiella pneumoniae (K. pneumoniae) and Escherichia coli (E. coli) isolated in Cambodia between 2016 and 2020.

Methods

E. coli (n = 223) and K. pneumoniae (n = 39) were tested for colistin minimum inhibitory concentration (MIC) by broth microdilution. Resistant isolates were subjected to polymerase chain reaction (PCR) for detection of mobile colistin resistance genes (mcr) and chromosomal mutations in the two-component system (TCS).

Results

Eighteen isolates (10 K. pneumoniae and 8 E. coli) revealed colistin resistance with a rate of 5.9% in E. coli and 34.8% in K. pneumoniae among ESBL isolates, and 1% in E. coli and 12.5% in K. pneumoniae among CP isolates. The resistance was associated with mcr variants (13/18 isolates, mcr-1, mcr-3, and mcr-8.2) and TCS mutations within E. coli and K. pneumoniae, with the first detection of mcr-8.2 in Cambodia, the discovery of new mutations potentially associated to colistin resistance in the TCS of E. coli (PhoP I47V, PhoQ N352K, PmrB G19R, and PmrD G85R) and the co-occurrence of mcr genes and colistin resistance conferring TCS mutations in 11 of 18 isolates.

Conclusions

The findings highlight the presence of colistin resistance in ESBL- and CP- Enterobacteriaceae involved in human infections in Cambodia as well as chromosomal mutations in TCS and the emergence of mcr-8.2 in E. coli and K. pneumoniae. It underscores the need for continuous surveillance, antimicrobial stewardship, and control measures to mitigate the spread of colistin resistance.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信