喀拉海海底沉积物上层甲烷循环微生物群落的组成

IF 0.7 4区 地球科学 Q4 GEOCHEMISTRY & GEOPHYSICS
A. L. Bryukhanov, V. S. Sevastyanov, M. D. Kravchishina, S. A. Voropaev, N. V. Dushenko, A. V. Kurakov, V. Yu. Fedulova
{"title":"喀拉海海底沉积物上层甲烷循环微生物群落的组成","authors":"A. L. Bryukhanov,&nbsp;V. S. Sevastyanov,&nbsp;M. D. Kravchishina,&nbsp;S. A. Voropaev,&nbsp;N. V. Dushenko,&nbsp;A. V. Kurakov,&nbsp;V. Yu. Fedulova","doi":"10.1134/S0016702924700277","DOIUrl":null,"url":null,"abstract":"<div><p>A study of the distribution of methane cycle microbial communities in the upper layers of bottom sediments above large hydrocarbon reservoirs in the South Kara petroleum region of the West Siberian Province revealed the presence in these layers of both aerobic methanotrophic bacteria and anaerobic methanogenic archaea, as well as numerous heterotrophic microorganisms of various phylogenetic groups. Research was carried out in the Baydaratskaya Bay and in the east of the Pukhuchan Depression (southern part of the Kara Sea). Aerobic methanotrophic bacteria belonged to the families <i>Methyloligellaceae</i>, <i>Methylophagaceae</i> and <i>Methylomonaceae</i> were detected in the surface oxidized layers (0–2 cm, <i>E</i><sub><i>h</i></sub> from 60 to 175 mV) of bottom sediments. Moreover, representatives of <i>Methyloligellaceae</i> were found in quite significant amount (1.52–2.61% of all 16S rRNA gene sequence reads) at all studied stations of the Kara Sea. In the subsurface reduced layers (18–20 cm, <i>E</i><sub><i>h</i></sub> from –63 to –246 mV), methanogenic archaea were dominated by representatives of the order <i>Methanomassiliicoccales</i> (up to 3.3% of all 16S rRNA gene sequence reads). Methanogenic archaea of the orders <i>Methanofastidiosales</i>, <i>Methanobacteriales</i> and <i>Methanomicrobiales</i> were also discovered. In addition, aerobic/facultative anaerobic methanotrophic bacteria of the families <i>Methylococcaceae</i> and <i>Methylomonadaceae</i> were found on these reduced layers of the bottom sediments, but the relative abundance (in percentage of sequence reads of their total number) of anaerobic methanotrophic archaea was extremely low there. Apparently, the oxidation of methane in bottom sediments of the Kara Sea, where its concentration at most of the studied stations dramatically increased with sediment depth, occurs predominantly under aerobic conditions in the surface layers, where the relative abundance and phylogenetic diversity of methanotrophs are higher.</p></div>","PeriodicalId":12781,"journal":{"name":"Geochemistry International","volume":"62 6","pages":"609 - 617"},"PeriodicalIF":0.7000,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Composition of Methane Cycle Microbial Communities in the Upper Layers of Bottom Sediments of the Kara Sea\",\"authors\":\"A. L. Bryukhanov,&nbsp;V. S. Sevastyanov,&nbsp;M. D. Kravchishina,&nbsp;S. A. Voropaev,&nbsp;N. V. Dushenko,&nbsp;A. V. Kurakov,&nbsp;V. Yu. Fedulova\",\"doi\":\"10.1134/S0016702924700277\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A study of the distribution of methane cycle microbial communities in the upper layers of bottom sediments above large hydrocarbon reservoirs in the South Kara petroleum region of the West Siberian Province revealed the presence in these layers of both aerobic methanotrophic bacteria and anaerobic methanogenic archaea, as well as numerous heterotrophic microorganisms of various phylogenetic groups. Research was carried out in the Baydaratskaya Bay and in the east of the Pukhuchan Depression (southern part of the Kara Sea). Aerobic methanotrophic bacteria belonged to the families <i>Methyloligellaceae</i>, <i>Methylophagaceae</i> and <i>Methylomonaceae</i> were detected in the surface oxidized layers (0–2 cm, <i>E</i><sub><i>h</i></sub> from 60 to 175 mV) of bottom sediments. Moreover, representatives of <i>Methyloligellaceae</i> were found in quite significant amount (1.52–2.61% of all 16S rRNA gene sequence reads) at all studied stations of the Kara Sea. In the subsurface reduced layers (18–20 cm, <i>E</i><sub><i>h</i></sub> from –63 to –246 mV), methanogenic archaea were dominated by representatives of the order <i>Methanomassiliicoccales</i> (up to 3.3% of all 16S rRNA gene sequence reads). Methanogenic archaea of the orders <i>Methanofastidiosales</i>, <i>Methanobacteriales</i> and <i>Methanomicrobiales</i> were also discovered. In addition, aerobic/facultative anaerobic methanotrophic bacteria of the families <i>Methylococcaceae</i> and <i>Methylomonadaceae</i> were found on these reduced layers of the bottom sediments, but the relative abundance (in percentage of sequence reads of their total number) of anaerobic methanotrophic archaea was extremely low there. Apparently, the oxidation of methane in bottom sediments of the Kara Sea, where its concentration at most of the studied stations dramatically increased with sediment depth, occurs predominantly under aerobic conditions in the surface layers, where the relative abundance and phylogenetic diversity of methanotrophs are higher.</p></div>\",\"PeriodicalId\":12781,\"journal\":{\"name\":\"Geochemistry International\",\"volume\":\"62 6\",\"pages\":\"609 - 617\"},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2024-07-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Geochemistry International\",\"FirstCategoryId\":\"89\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S0016702924700277\",\"RegionNum\":4,\"RegionCategory\":\"地球科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"GEOCHEMISTRY & GEOPHYSICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Geochemistry International","FirstCategoryId":"89","ListUrlMain":"https://link.springer.com/article/10.1134/S0016702924700277","RegionNum":4,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GEOCHEMISTRY & GEOPHYSICS","Score":null,"Total":0}
引用次数: 0

摘要

对西西伯利亚省南卡拉石油区大型碳氢化合物储层上方底层沉积物上层甲烷循环微生物群落分布情况的研究表明,这些层中既有需氧甲烷营养细菌和厌氧甲烷古细菌,也有不同系统发育群的大量异养微生物。研究是在巴伊达拉茨卡亚湾和普呼寒洼地东部(喀拉海南部)进行的。在底层沉积物的表层氧化层(0-2 厘米,Eh 值为 60 至 175 毫伏)检测到了属于甲藻科、甲藻属和甲单胞菌科的需氧甲烷营养细菌。此外,在喀拉海所有研究站位都发现了相当数量的甲藻科代表(占所有 16S rRNA 基因序列读数的 1.52-2.61%)。在表层下的还原层(18-20 厘米,Eh 值在 -63 至 -246 mV 之间),产甲烷古菌主要是甲烷纤球纲的代表菌(占所有 16S rRNA 基因序列读数的 3.3%)。此外,还发现了 Methanofastidiosales、Methanobacteriales 和 Methanomicrobiales 目甲烷古菌。此外,在这些底层沉积物的还原层中还发现了甲球菌科和甲单胞菌科的需氧/兼性厌氧甲烷营养细菌,但厌氧甲烷营养古细菌的相对丰度(以序列读数占其总数的百分比表示)极低。显然,甲烷在喀拉海底层沉积物中的氧化过程主要是在表层的好氧条件下进行的,而在表层,甲烷滋养生物的相对丰度和系统发育多样性都较高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Composition of Methane Cycle Microbial Communities in the Upper Layers of Bottom Sediments of the Kara Sea

Composition of Methane Cycle Microbial Communities in the Upper Layers of Bottom Sediments of the Kara Sea

Composition of Methane Cycle Microbial Communities in the Upper Layers of Bottom Sediments of the Kara Sea

A study of the distribution of methane cycle microbial communities in the upper layers of bottom sediments above large hydrocarbon reservoirs in the South Kara petroleum region of the West Siberian Province revealed the presence in these layers of both aerobic methanotrophic bacteria and anaerobic methanogenic archaea, as well as numerous heterotrophic microorganisms of various phylogenetic groups. Research was carried out in the Baydaratskaya Bay and in the east of the Pukhuchan Depression (southern part of the Kara Sea). Aerobic methanotrophic bacteria belonged to the families Methyloligellaceae, Methylophagaceae and Methylomonaceae were detected in the surface oxidized layers (0–2 cm, Eh from 60 to 175 mV) of bottom sediments. Moreover, representatives of Methyloligellaceae were found in quite significant amount (1.52–2.61% of all 16S rRNA gene sequence reads) at all studied stations of the Kara Sea. In the subsurface reduced layers (18–20 cm, Eh from –63 to –246 mV), methanogenic archaea were dominated by representatives of the order Methanomassiliicoccales (up to 3.3% of all 16S rRNA gene sequence reads). Methanogenic archaea of the orders Methanofastidiosales, Methanobacteriales and Methanomicrobiales were also discovered. In addition, aerobic/facultative anaerobic methanotrophic bacteria of the families Methylococcaceae and Methylomonadaceae were found on these reduced layers of the bottom sediments, but the relative abundance (in percentage of sequence reads of their total number) of anaerobic methanotrophic archaea was extremely low there. Apparently, the oxidation of methane in bottom sediments of the Kara Sea, where its concentration at most of the studied stations dramatically increased with sediment depth, occurs predominantly under aerobic conditions in the surface layers, where the relative abundance and phylogenetic diversity of methanotrophs are higher.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Geochemistry International
Geochemistry International 地学-地球化学与地球物理
CiteScore
1.60
自引率
12.50%
发文量
89
审稿时长
1 months
期刊介绍: Geochemistry International is a peer reviewed journal that publishes articles on cosmochemistry; geochemistry of magmatic, metamorphic, hydrothermal, and sedimentary processes; isotope geochemistry; organic geochemistry; applied geochemistry; and chemistry of the environment. Geochemistry International provides readers with a unique opportunity to refine their understanding of the geology of the vast territory of the Eurasian continent. The journal welcomes manuscripts from all countries in the English or Russian language.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信