非生物胁迫条件下珍珠米三螺旋家族的全面基因组筛选和表达谱分析,重点关注 PgTHX24 的功能研究

IF 5.4 Q1 PLANT SCIENCES
Jeky Chanwala , Deepak Kumar Jha , Tsheten Sherpa , Khushbu Kumari , Preeti Barla , Abhimanyu Das , Nrisingha Dey
{"title":"非生物胁迫条件下珍珠米三螺旋家族的全面基因组筛选和表达谱分析,重点关注 PgTHX24 的功能研究","authors":"Jeky Chanwala ,&nbsp;Deepak Kumar Jha ,&nbsp;Tsheten Sherpa ,&nbsp;Khushbu Kumari ,&nbsp;Preeti Barla ,&nbsp;Abhimanyu Das ,&nbsp;Nrisingha Dey","doi":"10.1016/j.cpb.2024.100371","DOIUrl":null,"url":null,"abstract":"<div><p>The trihelix transcription factors (THX TFs) play a crucial role in light responses and are involved in plant growth, development, and stress responses. In this study, we have identified 35 trihelix TFs in pearl millet (<em>Pennisetum glaucum</em>), which is one of the most widely grown C<sub>4</sub> cereal crops in tropical semi-arid regions. Identified PgTHXs (Trihelix members of <em>P. glaucum</em>) were classified into 5 subgroups (GT1, GT2, GTγ, SH4, and SIP1) based on phylogenetic analysis, and these subgroup members shared similar gene structure and motif distribution pattern. Collinearity analysis exhibited gene duplication events of trihelix family members in pearl millet across the genome. Gene ontology (GO) annotation and <em>cis</em>-regulatory elements (CREs) analysis of <em>PgTHX</em>s suggested their involvement in diverse biological and molecular functions associated with plant growth, development, and stress responses. RNA sequencing data and expression profile displayed differential expression patterns of <em>PgTHX</em>s under abiotic stress and phytohormone treatments. The induced expression pattern of the <em>PgTHX4</em>, <em>PgTHX5</em>, <em>PgTHX24,</em> and <em>PgTHX30</em> suggested their potential involvement in abiotic stress responses through phytohormonal signalling pathways. Among these, <em>PgTHX24</em>, a GT-3b member, was localized in the nucleus with self-transactivation ability. Overexpression of <em>PgTHX24</em> positively regulated expression of stress-related markers in transformed pearl millet calli under drought stress conditions. Promoter activity analysis also highlighted the stress-inducible nature of <em>PgTHX24</em>’s promoter. Overall, our findings provide a comprehensive understanding of <em>PgTHX</em>s with a framework for further functional characterization to understand their regulatory role in pearl millet’s growth, development, and stress responses.</p></div><div><h3>Key message</h3><p>Thirty-five trihelix TFs were identified in pearl millet, and a comprehensive expression profile highlighted their functional diversity. Overexpression of <em>PgTHX24</em> exhibited its potential involvement in abiotic stress responses.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":null,"pages":null},"PeriodicalIF":5.4000,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214662824000537/pdfft?md5=80b9bac6adc4ae0d5cd00ffbc72b9070&pid=1-s2.0-S2214662824000537-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Comprehensive genomic screening and expression profiling of trihelix family in pearl millet under abiotic stresses with emphasis on functional insights of PgTHX24\",\"authors\":\"Jeky Chanwala ,&nbsp;Deepak Kumar Jha ,&nbsp;Tsheten Sherpa ,&nbsp;Khushbu Kumari ,&nbsp;Preeti Barla ,&nbsp;Abhimanyu Das ,&nbsp;Nrisingha Dey\",\"doi\":\"10.1016/j.cpb.2024.100371\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The trihelix transcription factors (THX TFs) play a crucial role in light responses and are involved in plant growth, development, and stress responses. In this study, we have identified 35 trihelix TFs in pearl millet (<em>Pennisetum glaucum</em>), which is one of the most widely grown C<sub>4</sub> cereal crops in tropical semi-arid regions. Identified PgTHXs (Trihelix members of <em>P. glaucum</em>) were classified into 5 subgroups (GT1, GT2, GTγ, SH4, and SIP1) based on phylogenetic analysis, and these subgroup members shared similar gene structure and motif distribution pattern. Collinearity analysis exhibited gene duplication events of trihelix family members in pearl millet across the genome. Gene ontology (GO) annotation and <em>cis</em>-regulatory elements (CREs) analysis of <em>PgTHX</em>s suggested their involvement in diverse biological and molecular functions associated with plant growth, development, and stress responses. RNA sequencing data and expression profile displayed differential expression patterns of <em>PgTHX</em>s under abiotic stress and phytohormone treatments. The induced expression pattern of the <em>PgTHX4</em>, <em>PgTHX5</em>, <em>PgTHX24,</em> and <em>PgTHX30</em> suggested their potential involvement in abiotic stress responses through phytohormonal signalling pathways. Among these, <em>PgTHX24</em>, a GT-3b member, was localized in the nucleus with self-transactivation ability. Overexpression of <em>PgTHX24</em> positively regulated expression of stress-related markers in transformed pearl millet calli under drought stress conditions. Promoter activity analysis also highlighted the stress-inducible nature of <em>PgTHX24</em>’s promoter. Overall, our findings provide a comprehensive understanding of <em>PgTHX</em>s with a framework for further functional characterization to understand their regulatory role in pearl millet’s growth, development, and stress responses.</p></div><div><h3>Key message</h3><p>Thirty-five trihelix TFs were identified in pearl millet, and a comprehensive expression profile highlighted their functional diversity. Overexpression of <em>PgTHX24</em> exhibited its potential involvement in abiotic stress responses.</p></div>\",\"PeriodicalId\":38090,\"journal\":{\"name\":\"Current Plant Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2024-07-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2214662824000537/pdfft?md5=80b9bac6adc4ae0d5cd00ffbc72b9070&pid=1-s2.0-S2214662824000537-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Plant Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214662824000537\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Plant Biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214662824000537","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

三螺旋转录因子(THX TFs)在光反应中起着至关重要的作用,并参与植物的生长、发育和胁迫反应。在这项研究中,我们鉴定了珍珠粟(Pennisetum glaucum)中的 35 个三螺旋转录因子,珍珠粟是热带半干旱地区最广泛种植的 C4 谷类作物之一。根据系统发育分析,被鉴定的 PgTHXs(珍珠粟中的三螺旋成员)被分为 5 个亚群(GT1、GT2、GTγ、SH4 和 SIP1),这些亚群成员具有相似的基因结构和基序分布模式。共线性分析表明,三螺旋家族成员的基因在珍珠粟整个基因组中存在重复事件。对 PgTHXs 的基因本体(GO)注释和顺式调控元件(CREs)分析表明,它们参与了与植物生长、发育和胁迫响应相关的多种生物和分子功能。RNA 测序数据和表达谱显示了 PgTHXs 在非生物胁迫和植物激素处理下的不同表达模式。PgTHX4、PgTHX5、PgTHX24和PgTHX30的诱导表达模式表明,它们可能通过植物激素信号途径参与非生物胁迫响应。其中,PgTHX24 是 GT-3b 成员,定位于细胞核,具有自激活能力。在干旱胁迫条件下,过表达 PgTHX24 能正向调控转化珍珠米胼胝体中胁迫相关标记的表达。启动子活性分析也突显了 PgTHX24 启动子的胁迫诱导特性。总之,我们的研究结果提供了一个全面了解 PgTHXs 的框架,以便进一步进行功能表征,了解它们在珍珠粟的生长、发育和胁迫响应中的调控作用。PgTHX24的过表达表明它可能参与了非生物胁迫响应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comprehensive genomic screening and expression profiling of trihelix family in pearl millet under abiotic stresses with emphasis on functional insights of PgTHX24

The trihelix transcription factors (THX TFs) play a crucial role in light responses and are involved in plant growth, development, and stress responses. In this study, we have identified 35 trihelix TFs in pearl millet (Pennisetum glaucum), which is one of the most widely grown C4 cereal crops in tropical semi-arid regions. Identified PgTHXs (Trihelix members of P. glaucum) were classified into 5 subgroups (GT1, GT2, GTγ, SH4, and SIP1) based on phylogenetic analysis, and these subgroup members shared similar gene structure and motif distribution pattern. Collinearity analysis exhibited gene duplication events of trihelix family members in pearl millet across the genome. Gene ontology (GO) annotation and cis-regulatory elements (CREs) analysis of PgTHXs suggested their involvement in diverse biological and molecular functions associated with plant growth, development, and stress responses. RNA sequencing data and expression profile displayed differential expression patterns of PgTHXs under abiotic stress and phytohormone treatments. The induced expression pattern of the PgTHX4, PgTHX5, PgTHX24, and PgTHX30 suggested their potential involvement in abiotic stress responses through phytohormonal signalling pathways. Among these, PgTHX24, a GT-3b member, was localized in the nucleus with self-transactivation ability. Overexpression of PgTHX24 positively regulated expression of stress-related markers in transformed pearl millet calli under drought stress conditions. Promoter activity analysis also highlighted the stress-inducible nature of PgTHX24’s promoter. Overall, our findings provide a comprehensive understanding of PgTHXs with a framework for further functional characterization to understand their regulatory role in pearl millet’s growth, development, and stress responses.

Key message

Thirty-five trihelix TFs were identified in pearl millet, and a comprehensive expression profile highlighted their functional diversity. Overexpression of PgTHX24 exhibited its potential involvement in abiotic stress responses.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信