Ruitian Hou , Qiwei Jiang , Meiling Cheng , Jun Dai , Huiqin Yang , Jiao Yuan , Xiao Li , Xiaoping Tang , Haisheng Yu
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引用次数: 0
摘要
2022 年全球爆发的猴痘病毒（MPXV）疫情凸显了对有效、易得的新一代疫苗和中和抗体的迫切需求。在此，我们利用高通量单细胞 RNA 和 V(D)J 测序技术，从猴痘康复期患者的抗原分选 B 细胞中鉴定出了 MPXV 中和抗体。从 34 个成对的重链和轻链 B 细胞受体序列中获得了表达 IgG1 的 B 细胞。随后，三种强效中和抗体（MV127、MV128 和 MV129）被鉴定出来，并与 MPXV A35 蛋白发生反应。其中，MV129 对正宗 MPXV 的半数最大抑制浓度为 2.68μg/mL，被认为是猴痘中和 MPXV 的可能候选抗体。
Identification of neutralizing antibodies against monkeypox virus using high-throughput sequencing of A35+H3L+B cells from patients with convalescent monkeypox
The global monkeypox virus (MPXV) outbreak in 2022 emphasizes the urgent need for effective and accessible new-generation vaccines and neutralizing antibodies. Herein, we identified MPXV-neutralizing antibodies using high-throughput single-cell RNA and V(D)J sequencing of antigen-sorted B cells from patients with convalescent monkeypox. IgG1-expressing B cells were obtained from 34 paired heavy- and light-chain B cell receptor sequences. Subsequently, three potent neutralizing antibodies, MV127, MV128, and MV129, were identified and reacted with the MPXV A35 protein. Among these, MV129, which has a half-maximal inhibitory concentration of 2.68μg/mL against authentic MPXV, was considered to be the putative candidates for MPXV neutralization in response to monkeypox disease.
期刊介绍：
Virus Research provides a means of fast publication for original papers on fundamental research in virology. Contributions on new developments concerning virus structure, replication, pathogenesis and evolution are encouraged. These include reports describing virus morphology, the function and antigenic analysis of virus structural components, virus genome structure and expression, analysis on virus replication processes, virus evolution in connection with antiviral interventions, effects of viruses on their host cells, particularly on the immune system, and the pathogenesis of virus infections, including oncogene activation and transduction.
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