De Peng, Liqun Li, Aosong Wei, Ling Zhou, Bingxin Wang, Mingliu Liu, Yanhong Lei, Yanzhou Xie, Xuejun Li
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引用次数: 0
摘要
主要结论:在转基因拟南芥中,TaMYB44-5A 被鉴定为负调控抗旱性的转录因子。干旱会使整个小麦生长季节的产量严重下降。许多研究表明,R2R3-MYB 转录因子参与了干旱胁迫反应。本研究鉴定了小麦(Triticum aestivum L.)中的 R2R3-MYB 转录因子 MYB44-5A,并对其进行了功能分析。分离出了 TaMYB44 的三个同源物,它们都定位于细胞核。过表达 TaMYB44-5A 会降低拟南芥的耐旱性。进一步的分析表明,TaMYB44-5A 降低了转基因拟南芥对 ABA 的敏感性。遗传和转录调控分析表明,TaMYB44-5A 下调了干旱和 ABA 响应基因的表达水平,TaMYB44-5A 直接与启动子上的 MYB 结合位点结合,抑制了 TaRD22-3A 的转录水平。我们的研究结果揭示了 R2R3-MYB 转录因子在干旱胁迫下负向调控 ABA 信号转导的新分子途径。
TaMYB44-5A reduces drought tolerance by repressing transcription of TaRD22-3A in the abscisic acid signaling pathway.
Main conclusion: TaMYB44-5A identified as a transcription factor negatively regulates drought tolerance in transgenic Arabidopsis. Drought can severely reduce yields throughout the wheat-growing season. Many studies have shown that R2R3-MYB transcription factors are involved in drought stress responses. In this study, the R2R3-MYB transcription factor MYB44-5A was identified in wheat (Triticum aestivum L.) and functionally analyzed. Three homologs of TaMYB44 were isolated, all of which localized to the nucleus. Overexpression of TaMYB44-5A reduced drought tolerance in Arabidopsis thaliana. Further analysis showed that TaMYB44-5A reduced the sensitivity of transgenic Arabidopsis to ABA. Genetic and transcriptional regulation analyses demonstrated that the expression levels of drought- and ABA-responsive genes were downregulated by TaMYB44-5A, and TaMYB44-5A directly bound to the MYB-binding site on the promoter to repress the transcription level of TaRD22-3A. Our results provide insights into a novel molecular pathway in which the R2R3-MYB transcription factor negatively regulates ABA signaling in response to drought stress.
期刊介绍:
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