Xingming Li , Min Fu , Yaxu Len , Renjing Hu , Changwen Xu , Xia Xiong , Yingshun Zhou
{"title":"ST29 和 K212 型耐多药高病毒性肺炎克雷伯氏菌携带 tmexC2-tmexD2-toprJ2 的特征。","authors":"Xingming Li , Min Fu , Yaxu Len , Renjing Hu , Changwen Xu , Xia Xiong , Yingshun Zhou","doi":"10.1016/j.jgar.2024.06.014","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>This study aimed to characterize a tigecycline-resistant hypervirulent <em>Klebsiella pneumoniae</em> (HvKP) strain, identified as KLZT, which carries the tigecycline resistance gene cluster <em>tmexC2-tmexD2-toprJ2</em> belonging to ST29 and serotype K212.</p></div><div><h3>Methods</h3><p>Antimicrobial susceptibility and virulence phenotypes were assessed, followed by whole-genome sequencing (WGS) using PacBio II and MiSeq sequencers. Genome annotation was carried out using the RAST server and bioinformatics analysis revealed the genetic characteristics of this strain.</p></div><div><h3>Results</h3><p>Antimicrobial and virulence phenotype testing indicated that <em>K. pneumoniae</em> strain KLZT could be considered as a multidrug-resistant HvKP. WGS analysis showed that KLZT has a single 5,536,506-bp chromosome containing three plasmids 290,963 bp (pKLZT-1), 199,302 bp (pKLZT-2), and 4820 bp (pKLZT-3) in size, and also includes the ST29 and K212 serotypes. Four (<em>bla</em><sub>SHV-187</sub>, <em>oqxA, oqxB</em>, and <em>fosA6</em>) and six resistance genes (<em>tmexC2-tmxeD2-toprJ2, bla</em><sub>OXA-1</sub>, <em>aac(6′)-Ib-cr, catB3, arr-3</em>, and <em>bla</em><sub>LEN27</sub>) were identified from chromosomal and plasmid pKLZT-1, respectively. Gene-based analysis of the resistance genes of plasmid pKLZT-1 showed that the tigecycline resistance gene cluster-carrying region was flanked by <em>umuC</em> and <em>umuD</em> (<em>umuD</em>-<em>hps</em>-<em>IS5</em>-<em>tmexC2-tmexD2-toprJ2</em>-<em>umuC</em>), as well as other resistance genes and virulence factors (<em>ureB, ureC</em>, and <em>ureG</em>), which were carried by <em>IS5075</em>-<em>Tn3-intI1 -aac(6′)-Ib-cr-bla</em><sub>OXA-1</sub><em>-catB3-arr-3-bla</em><sub>LEN27</sub><em>-Tn3-ISkpn26-ureBCG-IS5075</em>.</p></div><div><h3>Conclusions</h3><p>WGS has revealed that a multidrug-resistant strain, HvKP KLZT, belonging to ST29 with capsular serotype K212, contains a multidrug-resistance plasmid.</p></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"38 ","pages":"Pages 349-353"},"PeriodicalIF":3.7000,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S221371652400122X/pdfft?md5=4ff437c688ce99f06347d0313582ae8a&pid=1-s2.0-S221371652400122X-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Characteristics of multidrug-resistant hypervirulent Klebsiella pneumoniae strains ST29 and K212 harbouring tmexC2-tmexD2-toprJ2\",\"authors\":\"Xingming Li , Min Fu , Yaxu Len , Renjing Hu , Changwen Xu , Xia Xiong , Yingshun Zhou\",\"doi\":\"10.1016/j.jgar.2024.06.014\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><p>This study aimed to characterize a tigecycline-resistant hypervirulent <em>Klebsiella pneumoniae</em> (HvKP) strain, identified as KLZT, which carries the tigecycline resistance gene cluster <em>tmexC2-tmexD2-toprJ2</em> belonging to ST29 and serotype K212.</p></div><div><h3>Methods</h3><p>Antimicrobial susceptibility and virulence phenotypes were assessed, followed by whole-genome sequencing (WGS) using PacBio II and MiSeq sequencers. Genome annotation was carried out using the RAST server and bioinformatics analysis revealed the genetic characteristics of this strain.</p></div><div><h3>Results</h3><p>Antimicrobial and virulence phenotype testing indicated that <em>K. pneumoniae</em> strain KLZT could be considered as a multidrug-resistant HvKP. WGS analysis showed that KLZT has a single 5,536,506-bp chromosome containing three plasmids 290,963 bp (pKLZT-1), 199,302 bp (pKLZT-2), and 4820 bp (pKLZT-3) in size, and also includes the ST29 and K212 serotypes. Four (<em>bla</em><sub>SHV-187</sub>, <em>oqxA, oqxB</em>, and <em>fosA6</em>) and six resistance genes (<em>tmexC2-tmxeD2-toprJ2, bla</em><sub>OXA-1</sub>, <em>aac(6′)-Ib-cr, catB3, arr-3</em>, and <em>bla</em><sub>LEN27</sub>) were identified from chromosomal and plasmid pKLZT-1, respectively. Gene-based analysis of the resistance genes of plasmid pKLZT-1 showed that the tigecycline resistance gene cluster-carrying region was flanked by <em>umuC</em> and <em>umuD</em> (<em>umuD</em>-<em>hps</em>-<em>IS5</em>-<em>tmexC2-tmexD2-toprJ2</em>-<em>umuC</em>), as well as other resistance genes and virulence factors (<em>ureB, ureC</em>, and <em>ureG</em>), which were carried by <em>IS5075</em>-<em>Tn3-intI1 -aac(6′)-Ib-cr-bla</em><sub>OXA-1</sub><em>-catB3-arr-3-bla</em><sub>LEN27</sub><em>-Tn3-ISkpn26-ureBCG-IS5075</em>.</p></div><div><h3>Conclusions</h3><p>WGS has revealed that a multidrug-resistant strain, HvKP KLZT, belonging to ST29 with capsular serotype K212, contains a multidrug-resistance plasmid.</p></div>\",\"PeriodicalId\":15936,\"journal\":{\"name\":\"Journal of global antimicrobial resistance\",\"volume\":\"38 \",\"pages\":\"Pages 349-353\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-07-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S221371652400122X/pdfft?md5=4ff437c688ce99f06347d0313582ae8a&pid=1-s2.0-S221371652400122X-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of global antimicrobial resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S221371652400122X\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S221371652400122X","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Characteristics of multidrug-resistant hypervirulent Klebsiella pneumoniae strains ST29 and K212 harbouring tmexC2-tmexD2-toprJ2
Objectives
This study aimed to characterize a tigecycline-resistant hypervirulent Klebsiella pneumoniae (HvKP) strain, identified as KLZT, which carries the tigecycline resistance gene cluster tmexC2-tmexD2-toprJ2 belonging to ST29 and serotype K212.
Methods
Antimicrobial susceptibility and virulence phenotypes were assessed, followed by whole-genome sequencing (WGS) using PacBio II and MiSeq sequencers. Genome annotation was carried out using the RAST server and bioinformatics analysis revealed the genetic characteristics of this strain.
Results
Antimicrobial and virulence phenotype testing indicated that K. pneumoniae strain KLZT could be considered as a multidrug-resistant HvKP. WGS analysis showed that KLZT has a single 5,536,506-bp chromosome containing three plasmids 290,963 bp (pKLZT-1), 199,302 bp (pKLZT-2), and 4820 bp (pKLZT-3) in size, and also includes the ST29 and K212 serotypes. Four (blaSHV-187, oqxA, oqxB, and fosA6) and six resistance genes (tmexC2-tmxeD2-toprJ2, blaOXA-1, aac(6′)-Ib-cr, catB3, arr-3, and blaLEN27) were identified from chromosomal and plasmid pKLZT-1, respectively. Gene-based analysis of the resistance genes of plasmid pKLZT-1 showed that the tigecycline resistance gene cluster-carrying region was flanked by umuC and umuD (umuD-hps-IS5-tmexC2-tmexD2-toprJ2-umuC), as well as other resistance genes and virulence factors (ureB, ureC, and ureG), which were carried by IS5075-Tn3-intI1 -aac(6′)-Ib-cr-blaOXA-1-catB3-arr-3-blaLEN27-Tn3-ISkpn26-ureBCG-IS5075.
Conclusions
WGS has revealed that a multidrug-resistant strain, HvKP KLZT, belonging to ST29 with capsular serotype K212, contains a multidrug-resistance plasmid.
期刊介绍：
The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes.
JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR).
Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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