Synechococcus 对溶解有机磷键类的利用。

IF 3.5 3区 生物学 Q2 MICROBIOLOGY
Emily M Waggoner, Kahina Djaoudi, Julia M Diaz, Solange Duhamel
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引用次数: 0

摘要

溶解有机磷(DOP)包含具有磷酯、磷酸酐和磷碳键的化合物。虽然 DOP 对海洋微生物具有重要的营养价值,但对于广泛分布的蓝藻球藻来说,每种键类的生物利用率在很大程度上仍是未知数。本研究评估了来自开阔海洋和近海的 Synechococcus 菌株对特定键类 DOP 的利用情况。当提供磷酸盐、磷酸酐(3-聚磷酸盐、45-聚磷酸盐)或同时具有磷酸酐和磷酯键的 DOP 化合物(5'-三磷酸腺苷)时,两种菌株的生长率相当。在磷酸酯(6-磷酸葡萄糖、5'-单磷酸腺苷、双(4-甲基伞形酮基)磷酸酯)上的生长率各不相同,两种菌株都不在选定的磷碳化合物上生长。两株菌株都先水解 3-聚磷酸盐,然后是 5'-三磷酸腺苷,最后是 5'-单磷酸腺苷,表现出优先水解磷酸酐键的酶解作用。这些菌株的外蛋白组含有磷水解酶,再加上在磷酸盐缺乏的情况下,3-聚磷酸盐和 5'-三磷酸腺苷的无细胞水解作用增强,表明这些外蛋白对磷酸酐键的矿化作用活跃。Synechococcus 碱性磷酸酶具有广泛的底物特异性,包括对磷酸酐 3-多聚磷酸的活性,不同菌株之间的亲和力不同。总之,这些发现强调了具有磷酸酐键的化合物在球藻磷营养中的潜在重要作用,并突出了对 DOP 键类分子多样性的不同生长和酶反应,从而扩大了我们对海洋生态系统中微生物介导的 DOP 循环的了解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dissolved organic phosphorus bond-class utilization by Synechococcus.

Dissolved organic phosphorus (DOP) contains compounds with phosphoester, phosphoanhydride, and phosphorus-carbon bonds. While DOP holds significant nutritional value for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by Synechococcus strains from open and coastal oceans. Both strains exhibited comparable growth rates when provided phosphate, a phosphoanhydride [3-polyphosphate and 45-polyphosphate], or a DOP compound with both phosphoanhydride and phosphoester bonds (adenosine 5'-triphosphate). Growth rates on phosphoesters [glucose-6-phosphate, adenosine 5'-monophosphate, bis(4-methylumbelliferyl) phosphate] were variable, and neither strain grew on selected phosphorus-carbon compounds. Both strains hydrolyzed 3-polyphosphate, then adenosine 5'-triphosphate, and lastly adenosine 5'-monophosphate, exhibiting preferential enzymatic hydrolysis of phosphoanhydride bonds. The strains' exoproteomes contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5'-triphosphate under phosphate deficiency, suggests active mineralization of phosphoanhydride bonds by these exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity toward the phosphoanhydride 3-polyphosphate, with varying affinities between strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition and highlight varied growth and enzymatic responses to molecular diversity within DOP bond-classes, thereby expanding our understanding of microbially mediated DOP cycling in marine ecosystems.

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来源期刊
FEMS microbiology ecology
FEMS microbiology ecology 生物-微生物学
CiteScore
7.50
自引率
2.40%
发文量
132
审稿时长
3 months
期刊介绍: FEMS Microbiology Ecology aims to ensure efficient publication of high-quality papers that are original and provide a significant contribution to the understanding of microbial ecology. The journal contains Research Articles and MiniReviews on fundamental aspects of the ecology of microorganisms in natural soil, aquatic and atmospheric habitats, including extreme environments, and in artificial or managed environments. Research papers on pure cultures and in the areas of plant pathology and medical, food or veterinary microbiology will be published where they provide valuable generic information on microbial ecology. Papers can deal with culturable and non-culturable forms of any type of microorganism: bacteria, archaea, filamentous fungi, yeasts, protozoa, cyanobacteria, algae or viruses. In addition, the journal will publish Perspectives, Current Opinion and Controversy Articles, Commentaries and Letters to the Editor on topical issues in microbial ecology. - Application of ecological theory to microbial ecology - Interactions and signalling between microorganisms and with plants and animals - Interactions between microorganisms and their physicochemical enviornment - Microbial aspects of biogeochemical cycles and processes - Microbial community ecology - Phylogenetic and functional diversity of microbial communities - Evolutionary biology of microorganisms
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