在蓝光照射下,视网膜细胞中的 miR-325-3p 会降低α-晶体蛋白 A 的含量。

IF 3.7 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
{"title":"在蓝光照射下,视网膜细胞中的 miR-325-3p 会降低α-晶体蛋白 A 的含量。","authors":"","doi":"10.1016/j.mocell.2024.100091","DOIUrl":null,"url":null,"abstract":"<div><p>Exposure to blue light can lead to retinal degeneration, causing adverse effects on eye health. Although the loss of retinal cells due to blue light exposure has been observed, the precise molecular mechanisms underlying this process remain poorly understood. In this study, we investigate the role of alpha-crystallin A (CRYAA) in neuro-retinal degeneration and their regulation by blue light. We observed significant apoptotic cell death in both the retina of rats and the cultured neuro-retinal cells. The expressions of <em>Cryaa</em> mRNA and protein were significantly downregulated in the retina exposed to blue light. We identified that miR-325-3p reduces <em>Cryaa</em> mRNA and protein by binding to its 3′-untranslated region. Upregulation of miR-325-3p destabilized <em>Cryaa</em> mRNA and suppresses CRYAA, whereas downregulation of miR-325-3p increased both expressions. Blue light-induced neuro-retinal cell death was alleviated by CRYAA overexpression. These results highlight the critical role of <em>Cryaa</em> mRNA and miR-325-3p molecular axis in blue light-induced retinal degeneration. Consequently, targeting CRYAA and miR-325-3p presents a potential strategy for protecting against blue light-induced retinal degeneration.</p></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S101684782400116X/pdfft?md5=017abe8e4402241b5ce9d97d3299f88e&pid=1-s2.0-S101684782400116X-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Decrease of alpha-crystallin A by miR-325-3p in retinal cells under blue light exposure\",\"authors\":\"\",\"doi\":\"10.1016/j.mocell.2024.100091\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Exposure to blue light can lead to retinal degeneration, causing adverse effects on eye health. Although the loss of retinal cells due to blue light exposure has been observed, the precise molecular mechanisms underlying this process remain poorly understood. In this study, we investigate the role of alpha-crystallin A (CRYAA) in neuro-retinal degeneration and their regulation by blue light. We observed significant apoptotic cell death in both the retina of rats and the cultured neuro-retinal cells. The expressions of <em>Cryaa</em> mRNA and protein were significantly downregulated in the retina exposed to blue light. We identified that miR-325-3p reduces <em>Cryaa</em> mRNA and protein by binding to its 3′-untranslated region. Upregulation of miR-325-3p destabilized <em>Cryaa</em> mRNA and suppresses CRYAA, whereas downregulation of miR-325-3p increased both expressions. Blue light-induced neuro-retinal cell death was alleviated by CRYAA overexpression. These results highlight the critical role of <em>Cryaa</em> mRNA and miR-325-3p molecular axis in blue light-induced retinal degeneration. Consequently, targeting CRYAA and miR-325-3p presents a potential strategy for protecting against blue light-induced retinal degeneration.</p></div>\",\"PeriodicalId\":18795,\"journal\":{\"name\":\"Molecules and Cells\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S101684782400116X/pdfft?md5=017abe8e4402241b5ce9d97d3299f88e&pid=1-s2.0-S101684782400116X-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecules and Cells\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S101684782400116X\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecules and Cells","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S101684782400116X","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

暴露在蓝光下会导致视网膜变性,对眼睛健康造成不利影响。虽然人们已经观察到蓝光照射导致视网膜细胞的损失,但对这一过程的确切分子机制仍然知之甚少。在这项研究中,我们研究了阿尔法晶体蛋白 A(CRYAA)在神经视网膜变性中的作用以及蓝光对它们的调控。我们在大鼠视网膜和培养的神经视网膜细胞中都观察到了明显的细胞凋亡。在暴露于蓝光的视网膜中,Cryaa mRNA 和蛋白质的表达均显著下调。我们发现,miR-325-3p 通过与其 3'- 非翻译区(UTR)结合,降低了 Cryaa mRNA 和蛋白质的表达。上调 miR-325-3p 会破坏 Cryaa mRNA 的稳定性并抑制 CRYAA,而下调 miR-325-3p 则会增加这两种表达。CRYAA的过表达缓解了蓝光诱导的神经视网膜细胞死亡。这些结果凸显了Cryaa mRNA和miR-325-3p分子轴在蓝光诱导的视网膜变性中的关键作用。因此,靶向CRYAA和miR-325-3p是防止蓝光诱导视网膜变性的一种潜在策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Decrease of alpha-crystallin A by miR-325-3p in retinal cells under blue light exposure

Exposure to blue light can lead to retinal degeneration, causing adverse effects on eye health. Although the loss of retinal cells due to blue light exposure has been observed, the precise molecular mechanisms underlying this process remain poorly understood. In this study, we investigate the role of alpha-crystallin A (CRYAA) in neuro-retinal degeneration and their regulation by blue light. We observed significant apoptotic cell death in both the retina of rats and the cultured neuro-retinal cells. The expressions of Cryaa mRNA and protein were significantly downregulated in the retina exposed to blue light. We identified that miR-325-3p reduces Cryaa mRNA and protein by binding to its 3′-untranslated region. Upregulation of miR-325-3p destabilized Cryaa mRNA and suppresses CRYAA, whereas downregulation of miR-325-3p increased both expressions. Blue light-induced neuro-retinal cell death was alleviated by CRYAA overexpression. These results highlight the critical role of Cryaa mRNA and miR-325-3p molecular axis in blue light-induced retinal degeneration. Consequently, targeting CRYAA and miR-325-3p presents a potential strategy for protecting against blue light-induced retinal degeneration.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Molecules and Cells
Molecules and Cells 生物-生化与分子生物学
CiteScore
6.60
自引率
10.50%
发文量
83
审稿时长
2.3 months
期刊介绍: Molecules and Cells is an international on-line open-access journal devoted to the advancement and dissemination of fundamental knowledge in molecular and cellular biology. It was launched in 1990 and ISO abbreviation is ''Mol. Cells''. Reports on a broad range of topics of general interest to molecular and cell biologists are published. It is published on the last day of each month by the Korean Society for Molecular and Cellular Biology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信