优化小鼠骨质增生的三重生长因子策略。

IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL
Taichi Tenkumo, Rie Koide, Toru Ogawa, Hirofumi Yamaguchi, Shigeki Suzuki, Makiko Miyashita, Keisuke Nakamura, Han Wang, Nobuhiro Yoda, Keiichi Sasaki
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引用次数: 0

摘要

随着牙科植入治疗成为黄金标准,在植入前进行有效的骨增量治疗的需求日益增长。本研究旨在评估一种整合了骨形态发生蛋白-2(BMP-2)、胰岛素样生长因子1(IGF-1)和血管内皮生长因子(VEGF)这三种关键生长因子的骨增量策略。我们制作了含有 BMP-2、IGF-1 或血管内皮生长因子的胶原支架,并根据其含量将其分为五组:单独的支架;单独的 BMP-2(BMP-2);BMP-2 和 IGF-1(BI);BMP-2、IGF-1 和血管内皮生长因子(BIV);以及 BMP-2 和 IGF-1 并提前释放血管内皮生长因子(BI + V)。将制备好的支架通过手术植入 C57BL/6JJcl 小鼠的小腿,10 天和 28 天后通过组织学、断层扫描和生化分析评估硬组织的形成。与单独使用 BMP-2 相比,无论是否补充 VEGF,BMP-2 和 IGF-1 的组合都能诱导更大体积的硬组织增生。BIV 组的硬组织形成量最大。相比之下,BI + V 组的硬组织体积与 BI 组相似。虽然 BIV 组的血管内皮生长因子和 CD31 水平在 10 天时最高,但在同一时间点,硬组织的形成与 M2 巨噬细胞的数量没有相关性。总之,同时释放 BMP-2、IGF-1 和 VEGF 被证明能有效促进骨增量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A triple growth factor strategy for optimizing bone augmentation in mice

With dental implant treatment becoming the gold standard, the need for effective bone augmentation prior to implantation has grown. This study aims to evaluate a bone augmentation strategy integrating three key growth factors: bone morphogenetic protein-2 (BMP-2), insulin-like growth factor 1 (IGF-1), and vascular endothelial growth factor (VEGF). Collagen scaffolds incorporating BMP-2, IGF-1, or VEGF were fabricated and categorized into five groups based on their content: scaffold alone; BMP-2 alone (BMP-2); BMP-2 and IGF-1 (BI); BMP-2, IGF-1, and VEGF (BIV); and BMP-2 and IGF-1 with an earlier release of VEGF (BI + V). The prepared scaffolds were surgically implanted into the calvarias of C57BL/6JJcl mice, and hard tissue formation was assessed after 10 and 28 days through histological, tomographic, and biochemical analyses. The combination of BMP-2 and IGF-1 induced a greater volume of hard tissue augmentation compared with that of BMP-2 alone, regardless of VEGF supplementation, and these groups had increased levels of cartilage compared with others. The volume of hard tissue formation was greatest in the BIV group. In contrast, the BI + V group exhibited a hard tissue volume similar to that of the BI group. While VEGF and CD31 levels were highest in the BIV group at 10 days, there was no correlation at the same time point between hard tissue formation and the quantity of M2 macrophages. In conclusion, the simultaneous release of BMP-2, IGF-1, and VEGF proved to be effective in promoting bone augmentation.

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来源期刊
CiteScore
7.50
自引率
2.90%
发文量
199
审稿时长
12 months
期刊介绍: Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
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