发掘黑麦草提取物的治疗潜力:植物化学分析揭示抗菌和抗氧化奇迹。

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Anees Ur Rahman, Abdullah Abdullah, Shah Faisal, Basem Mansour, Galal Yahya
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引用次数: 0

摘要

全球抗菌药耐药性的威胁日益严重,危及人类和动物的生命,因此迫切需要发现新型抗菌解决方案。药用植物有望成为潜在的抗菌化合物来源。在这项研究中,我们调查了黑木耳(黑种草)乙醇提取物的植物化学成分和杀菌能力。气相色谱分析(GC)确定了 11 种化合物,其中百里香醌和百里香酚具有抗菌和抗氧化特性。抗菌试验表明,它对铜绿假单胞菌、大肠杆菌、伤寒沙门氏菌、金黄色葡萄球菌、肠杆菌和枯草杆菌等多种细菌具有显著的抑菌作用,对黑曲霉、青霉和白色念珠菌也有很强的抗真菌活性。值得注意的是,当黑木耳提取物与抗生素结合使用时,会显示出卓越的协同抗菌功效。黑籽提取物具有膜破坏活性,能破坏保护微生物免受抗菌剂侵害的毒力因子,包括细菌生物膜的形成和蛋白酶的分泌。提取物的主要活性成分胸腺醌也具有类似的抗菌和抗病毒特性。针对铜绿假单胞菌中法定量感应和生物膜形成的关键调节因子(如 RhlG、LasR 和 PqsR)进行的硅学分析表明,百里酚和胸腺醌对这些靶标具有显著的亲和力。此外,N. sativa 提取物对原生型和非原生型的热带利什曼病寄生虫都表现出剂量依赖性细胞毒性,暗示了其潜在的抗寄生虫活性。除抗菌特性外,该提取物在浓度为 400 μg/mL 时还显示出潜在的抗氧化活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unlocking the therapeutic potential of Nigella sativa extract: phytochemical analysis and revealing antimicrobial and antioxidant marvels.

The growing global threat of antimicrobial resistance endangers both human and animal life, necessitating the urgent discovery of novel antimicrobial solutions. Medicinal plants hold promise as sources of potential antimicrobial compounds. In this study, we investigated the phytochemical constituents and microbicidal capabilities of the ethanolic extract from Nigella sativa (black seed). Gas chromatography analysis (GC) identified 11 compounds, among them thymoquinone, and thymol, contributing to antimicrobial and antioxidant properties. Antimicrobial assays demonstrated notable inhibition zones against broad spectra of bacteria, including Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi, Staphylococcus aureus, Enterobacter, and Bacillus subtilis, along with potent antifungal activity against Aspergillus niger, Penicillium, and Candida albicans. Notably, when combined with antibiotics, the extract displayed exceptional synergistic antimicrobial efficacy. The black seed extract demonstrated membrane-damaging activity and disrupted virulence factors that protect microbes from antimicrobial agents, including the formation of bacterial biofilm and protease secretion. Thymoquinone, the primary active constituent of the extract, exhibited similar antimicrobial and ant virulence properties. In silico analysis targeting key regulators of quorum sensing and biofilm formation in P. aeruginosa, such as RhlG, LasR, and PqsR, showed a remarkable affinity of thymol and thymoquinone for these targets. Moreover, the N. sativa extract exhibited dose-dependent cytotoxicity against both the promastigote and amastigote forms of Leishmania tropica parasites, hinting at potential antiparasitic activity. In addition to its antimicrobial properties, the extract displayed potential antioxidant activity at a concentration of 400 μg/mL.

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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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