Tolulope A. Oyewole , Nurat O. Mohammed , Bright O. Osarenren , Muyideen K. Tijani , Kristina E.M. Persson , Mofolusho O. Falade
{"title":"尼日利亚伊巴丹市基于裂头蚴表面蛋白 1 (msp1) 和 2 (msp2) 基因多样性和抗体反应的恶性疟原虫传播情况","authors":"Tolulope A. Oyewole , Nurat O. Mohammed , Bright O. Osarenren , Muyideen K. Tijani , Kristina E.M. Persson , Mofolusho O. Falade","doi":"10.1016/j.parepi.2024.e00366","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Nigeria is a major contributor to the global malaria burden. The genetic diversity of malaria parasite populations as well as antibody responses of individuals in affected areas against antigens of the parasite can reveal the transmission intensity, a key information required to control the disease. This work was carried out to determine the allelic frequency of highly polymorphic <em>Plasmodium falciparum</em> genes and antibody responses against schizont crude antigens in an area of Ibadan, Nigeria.</p></div><div><h3>Materials and methods</h3><p>Blood was collected from 147 individuals with symptoms suspected to be malaria. Malaria infection was determined using a rapid diagnostic test (RDT), and <em>msp1</em> and <em>msp2</em> were genotyped by a nested PCR method. In addition, levels of IgG directed against <em>P. falciparum</em> FCR3S1.2 schizont extract was measured in ELISA.</p></div><div><h3>Results</h3><p>Approximately 25% (36/147) were positive for a <em>P. falciparum</em> infection in RDT, but only 32 of the positive samples were successfully genotyped. MAD20 was the most prevalent and K1 the least prevalent of the <em>msp1</em> alleles. For <em>msp2</em>, FC27 was more prevalent than 3D7. The mean multiplicities of infection (MOI) were 1.9 and 1.7 for <em>msp1</em> and <em>msp2</em>, respectively. IgG levels correlated positively with age, however there was no difference in median antibody levels between RDT-positive and RDT-negative individuals.</p></div><div><h3>Conclusion</h3><p>Low MOI has before been correlated with low/intermediate transmission intensity, however, in this study, similar levels of <em>P. falciparum</em>-specific antibodies between infected and non-infected individuals point more towards a high level of exposure and a need for further measures to control the spread of malaria in this area.</p></div>","PeriodicalId":37873,"journal":{"name":"Parasite Epidemiology and Control","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405673124000308/pdfft?md5=f57dfc101127ce8f9d117e5332045676&pid=1-s2.0-S2405673124000308-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Plasmodium falciparum transmission based on merozoite surface protein 1 (msp1) and 2 (msp2) gene diversity and antibody responses in Ibadan, Nigeria\",\"authors\":\"Tolulope A. Oyewole , Nurat O. Mohammed , Bright O. Osarenren , Muyideen K. Tijani , Kristina E.M. Persson , Mofolusho O. Falade\",\"doi\":\"10.1016/j.parepi.2024.e00366\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Nigeria is a major contributor to the global malaria burden. The genetic diversity of malaria parasite populations as well as antibody responses of individuals in affected areas against antigens of the parasite can reveal the transmission intensity, a key information required to control the disease. This work was carried out to determine the allelic frequency of highly polymorphic <em>Plasmodium falciparum</em> genes and antibody responses against schizont crude antigens in an area of Ibadan, Nigeria.</p></div><div><h3>Materials and methods</h3><p>Blood was collected from 147 individuals with symptoms suspected to be malaria. Malaria infection was determined using a rapid diagnostic test (RDT), and <em>msp1</em> and <em>msp2</em> were genotyped by a nested PCR method. In addition, levels of IgG directed against <em>P. falciparum</em> FCR3S1.2 schizont extract was measured in ELISA.</p></div><div><h3>Results</h3><p>Approximately 25% (36/147) were positive for a <em>P. falciparum</em> infection in RDT, but only 32 of the positive samples were successfully genotyped. MAD20 was the most prevalent and K1 the least prevalent of the <em>msp1</em> alleles. For <em>msp2</em>, FC27 was more prevalent than 3D7. The mean multiplicities of infection (MOI) were 1.9 and 1.7 for <em>msp1</em> and <em>msp2</em>, respectively. IgG levels correlated positively with age, however there was no difference in median antibody levels between RDT-positive and RDT-negative individuals.</p></div><div><h3>Conclusion</h3><p>Low MOI has before been correlated with low/intermediate transmission intensity, however, in this study, similar levels of <em>P. falciparum</em>-specific antibodies between infected and non-infected individuals point more towards a high level of exposure and a need for further measures to control the spread of malaria in this area.</p></div>\",\"PeriodicalId\":37873,\"journal\":{\"name\":\"Parasite Epidemiology and Control\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-07-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2405673124000308/pdfft?md5=f57dfc101127ce8f9d117e5332045676&pid=1-s2.0-S2405673124000308-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Parasite Epidemiology and Control\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405673124000308\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Parasite Epidemiology and Control","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405673124000308","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Plasmodium falciparum transmission based on merozoite surface protein 1 (msp1) and 2 (msp2) gene diversity and antibody responses in Ibadan, Nigeria
Background
Nigeria is a major contributor to the global malaria burden. The genetic diversity of malaria parasite populations as well as antibody responses of individuals in affected areas against antigens of the parasite can reveal the transmission intensity, a key information required to control the disease. This work was carried out to determine the allelic frequency of highly polymorphic Plasmodium falciparum genes and antibody responses against schizont crude antigens in an area of Ibadan, Nigeria.
Materials and methods
Blood was collected from 147 individuals with symptoms suspected to be malaria. Malaria infection was determined using a rapid diagnostic test (RDT), and msp1 and msp2 were genotyped by a nested PCR method. In addition, levels of IgG directed against P. falciparum FCR3S1.2 schizont extract was measured in ELISA.
Results
Approximately 25% (36/147) were positive for a P. falciparum infection in RDT, but only 32 of the positive samples were successfully genotyped. MAD20 was the most prevalent and K1 the least prevalent of the msp1 alleles. For msp2, FC27 was more prevalent than 3D7. The mean multiplicities of infection (MOI) were 1.9 and 1.7 for msp1 and msp2, respectively. IgG levels correlated positively with age, however there was no difference in median antibody levels between RDT-positive and RDT-negative individuals.
Conclusion
Low MOI has before been correlated with low/intermediate transmission intensity, however, in this study, similar levels of P. falciparum-specific antibodies between infected and non-infected individuals point more towards a high level of exposure and a need for further measures to control the spread of malaria in this area.
期刊介绍：
Parasite Epidemiology and Control is an Open Access journal. There is an increasing amount of research in the parasitology area that analyses the patterns, causes, and effects of health and disease conditions in defined populations. This epidemiology of parasite infectious diseases is predominantly studied in human populations but also spans other major hosts of parasitic infections and as such this journal will have a broad remit. We will focus on the major areas of epidemiological study including disease etiology, disease surveillance, drug resistance and geographical spread and screening, biomonitoring, and comparisons of treatment effects in clinical trials for both human and other animals. We will also look at the epidemiology and control of vector insects. The journal will also cover the use of geographic information systems (Epi-GIS) for epidemiological surveillance which is a rapidly growing area of research in infectious diseases. Molecular epidemiological approaches are also particularly encouraged.
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