Maizura Ithnin , Wendy T. Vu , Marhalil Marjuni , Mohd Din Amiruddin , Anupam Singh , Vasantika Suryawanshi , Norhalida Mohamed Serdari , Noorhariza Mohd Zaki , Sergey V. Nuzhdin , Peter L. Chang , Rajinder Singh
{"title":"油橄榄物种特异性 SNP 可鉴定野生油橄榄种质资源中的天然杂交种","authors":"Maizura Ithnin , Wendy T. Vu , Marhalil Marjuni , Mohd Din Amiruddin , Anupam Singh , Vasantika Suryawanshi , Norhalida Mohamed Serdari , Noorhariza Mohd Zaki , Sergey V. Nuzhdin , Peter L. Chang , Rajinder Singh","doi":"10.1016/j.bcab.2024.103304","DOIUrl":null,"url":null,"abstract":"<div><p>The study identified a set of species-specific SNPs (ssSNPs) distinguishing between American populations of <em>Elaeis oleifera</em> and African populations of <em>Elaeis guineensis</em>. These ssSNPs exhibited the expected proportions of <em>E. oleifera</em> and <em>E. guineensis</em> alleles in the first generation of <em>E. oleifera</em> and <em>E. guineensis</em> hybrids (OxGF1) as well as in backcross 1 (BC1) and backcross 2 (BC2) populations. Application of the ssSNPs across 12 natural <em>Elaeis</em> hybrids identified in the <em>E. oleifera</em> germplasm collection previously assembled from South and Central America revealed allelic proportions similar to OxGF1 and backcrosses (BC). Inbreeding coefficients (Fis) in the 12 natural hybrids were within the range usually found in OxGF1 and backcross hybrids, below the pure, wild oleiferas. In addition, phenotypic evaluation (pollen shape and leaf planation) of selected natural hybrids identified using ssSNPs confirmed that the observed morphology was generally similar to laboratory-bred OxGF1 and backcross populations. Introduction of <em>E. guineensis</em> for commercial purposes into the American oil palm collection is an active component of the hybrid breeding program and could have inadvertently resulted in natural hybrids in the germplasm collection. We also identified forty-four palms with high sequence polymorphism to be shortlisted for conservation, which can cumulatively preserve 90% of the diversity present in the <em>E. oleifera</em> germplasm collection.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Elaeis species specific SNPs allow authentication of natural hybrids in a wild E. oleifera germplasm collection\",\"authors\":\"Maizura Ithnin , Wendy T. Vu , Marhalil Marjuni , Mohd Din Amiruddin , Anupam Singh , Vasantika Suryawanshi , Norhalida Mohamed Serdari , Noorhariza Mohd Zaki , Sergey V. Nuzhdin , Peter L. Chang , Rajinder Singh\",\"doi\":\"10.1016/j.bcab.2024.103304\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The study identified a set of species-specific SNPs (ssSNPs) distinguishing between American populations of <em>Elaeis oleifera</em> and African populations of <em>Elaeis guineensis</em>. These ssSNPs exhibited the expected proportions of <em>E. oleifera</em> and <em>E. guineensis</em> alleles in the first generation of <em>E. oleifera</em> and <em>E. guineensis</em> hybrids (OxGF1) as well as in backcross 1 (BC1) and backcross 2 (BC2) populations. Application of the ssSNPs across 12 natural <em>Elaeis</em> hybrids identified in the <em>E. oleifera</em> germplasm collection previously assembled from South and Central America revealed allelic proportions similar to OxGF1 and backcrosses (BC). Inbreeding coefficients (Fis) in the 12 natural hybrids were within the range usually found in OxGF1 and backcross hybrids, below the pure, wild oleiferas. In addition, phenotypic evaluation (pollen shape and leaf planation) of selected natural hybrids identified using ssSNPs confirmed that the observed morphology was generally similar to laboratory-bred OxGF1 and backcross populations. Introduction of <em>E. guineensis</em> for commercial purposes into the American oil palm collection is an active component of the hybrid breeding program and could have inadvertently resulted in natural hybrids in the germplasm collection. We also identified forty-four palms with high sequence polymorphism to be shortlisted for conservation, which can cumulatively preserve 90% of the diversity present in the <em>E. oleifera</em> germplasm collection.</p></div>\",\"PeriodicalId\":8774,\"journal\":{\"name\":\"Biocatalysis and agricultural biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-06-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biocatalysis and agricultural biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1878818124002883\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biocatalysis and agricultural biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1878818124002883","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Elaeis species specific SNPs allow authentication of natural hybrids in a wild E. oleifera germplasm collection
The study identified a set of species-specific SNPs (ssSNPs) distinguishing between American populations of Elaeis oleifera and African populations of Elaeis guineensis. These ssSNPs exhibited the expected proportions of E. oleifera and E. guineensis alleles in the first generation of E. oleifera and E. guineensis hybrids (OxGF1) as well as in backcross 1 (BC1) and backcross 2 (BC2) populations. Application of the ssSNPs across 12 natural Elaeis hybrids identified in the E. oleifera germplasm collection previously assembled from South and Central America revealed allelic proportions similar to OxGF1 and backcrosses (BC). Inbreeding coefficients (Fis) in the 12 natural hybrids were within the range usually found in OxGF1 and backcross hybrids, below the pure, wild oleiferas. In addition, phenotypic evaluation (pollen shape and leaf planation) of selected natural hybrids identified using ssSNPs confirmed that the observed morphology was generally similar to laboratory-bred OxGF1 and backcross populations. Introduction of E. guineensis for commercial purposes into the American oil palm collection is an active component of the hybrid breeding program and could have inadvertently resulted in natural hybrids in the germplasm collection. We also identified forty-four palms with high sequence polymorphism to be shortlisted for conservation, which can cumulatively preserve 90% of the diversity present in the E. oleifera germplasm collection.
期刊介绍:
Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.