Jianhua Yan , Wei Liu , Jiayu Wang , Hongjie Liu , Liwei Wang , Xian Li , Yixiang Li
{"title":"提高公共卫生安全:用于快速准确检测伤寒杆菌的 MoS2@CNT-Chit 电化学 DNA 生物传感器的开发与应用","authors":"Jianhua Yan , Wei Liu , Jiayu Wang , Hongjie Liu , Liwei Wang , Xian Li , Yixiang Li","doi":"10.1016/j.bej.2024.109416","DOIUrl":null,"url":null,"abstract":"<div><p>This study introduces an advanced electrochemical biosensor that utilizes MoS<sub>2</sub>@CNT as an electrode material combined with a specific DNA probe to detect <em>Salmonella Typhi</em> rapidly and accurately. The sensor offers a broad detection range from 1.0 × 10<sup>−6</sup> to 1.0 × 10<sup>−18</sup> molL<sup>−1</sup> and boasts an exceptionally low limit of detection (LOD) of 1.0 × 10<sup>−20</sup> molL<sup>−1</sup> for the target bacterium. It demonstrates a detection range from 1.0 × 10<sup>4</sup> to 1.0 × 10<sup>11</sup> CFUml<sup>−1</sup> in real samples, with a corresponding LOD of 1.0 × 10<sup>4</sup> CFUml<sup>−1</sup>. Rigorous testing against base mismatches and various bacterial strains confirms its specificity, ensuring reliable performance. Validated in real samples, the biosensor can accurately identify <em>Salmonella Typhi</em> in water and milk, achieving recoveries ranging from 92.95 % to 99.58 %. The exceptional performance of the biosensor is attributed to the MoS<sub>2</sub>@CNT electrode material and the specific DNA recognition probe, which enhance electron transfer and reduce steric impedance. These improvements contribute to the sensor's enhanced sensitivity and specificity, making it a significant advancement in public health safety by providing a rapid and accurate tool for detecting <em>Salmonella Typhi</em> in food samples.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhancing public health safety: Development and application of a MoS2@CNT-Chit electrochemical DNA biosensor for rapid and accurate detection of S. Typhi\",\"authors\":\"Jianhua Yan , Wei Liu , Jiayu Wang , Hongjie Liu , Liwei Wang , Xian Li , Yixiang Li\",\"doi\":\"10.1016/j.bej.2024.109416\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study introduces an advanced electrochemical biosensor that utilizes MoS<sub>2</sub>@CNT as an electrode material combined with a specific DNA probe to detect <em>Salmonella Typhi</em> rapidly and accurately. The sensor offers a broad detection range from 1.0 × 10<sup>−6</sup> to 1.0 × 10<sup>−18</sup> molL<sup>−1</sup> and boasts an exceptionally low limit of detection (LOD) of 1.0 × 10<sup>−20</sup> molL<sup>−1</sup> for the target bacterium. It demonstrates a detection range from 1.0 × 10<sup>4</sup> to 1.0 × 10<sup>11</sup> CFUml<sup>−1</sup> in real samples, with a corresponding LOD of 1.0 × 10<sup>4</sup> CFUml<sup>−1</sup>. Rigorous testing against base mismatches and various bacterial strains confirms its specificity, ensuring reliable performance. Validated in real samples, the biosensor can accurately identify <em>Salmonella Typhi</em> in water and milk, achieving recoveries ranging from 92.95 % to 99.58 %. The exceptional performance of the biosensor is attributed to the MoS<sub>2</sub>@CNT electrode material and the specific DNA recognition probe, which enhance electron transfer and reduce steric impedance. These improvements contribute to the sensor's enhanced sensitivity and specificity, making it a significant advancement in public health safety by providing a rapid and accurate tool for detecting <em>Salmonella Typhi</em> in food samples.</p></div>\",\"PeriodicalId\":8766,\"journal\":{\"name\":\"Biochemical Engineering Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-07-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemical Engineering Journal\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1369703X24002031\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1369703X24002031","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Enhancing public health safety: Development and application of a MoS2@CNT-Chit electrochemical DNA biosensor for rapid and accurate detection of S. Typhi
This study introduces an advanced electrochemical biosensor that utilizes MoS2@CNT as an electrode material combined with a specific DNA probe to detect Salmonella Typhi rapidly and accurately. The sensor offers a broad detection range from 1.0 × 10−6 to 1.0 × 10−18 molL−1 and boasts an exceptionally low limit of detection (LOD) of 1.0 × 10−20 molL−1 for the target bacterium. It demonstrates a detection range from 1.0 × 104 to 1.0 × 1011 CFUml−1 in real samples, with a corresponding LOD of 1.0 × 104 CFUml−1. Rigorous testing against base mismatches and various bacterial strains confirms its specificity, ensuring reliable performance. Validated in real samples, the biosensor can accurately identify Salmonella Typhi in water and milk, achieving recoveries ranging from 92.95 % to 99.58 %. The exceptional performance of the biosensor is attributed to the MoS2@CNT electrode material and the specific DNA recognition probe, which enhance electron transfer and reduce steric impedance. These improvements contribute to the sensor's enhanced sensitivity and specificity, making it a significant advancement in public health safety by providing a rapid and accurate tool for detecting Salmonella Typhi in food samples.
期刊介绍:
The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology.
The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields:
Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics
Biosensors and Biodevices including biofabrication and novel fuel cell development
Bioseparations including scale-up and protein refolding/renaturation
Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells
Bioreactor Systems including characterization, optimization and scale-up
Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization
Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals
Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release
Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites
Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation
Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis
Protein Engineering including enzyme engineering and directed evolution.