通过褪黑素/胆红素阳离子纳米颗粒提高卵母细胞体外成熟度和质量:辅助生殖技术的前景战略

IF 5.2 2区 医学 Q1 PHARMACOLOGY & PHARMACY
Haitao Xi , Lihui Huang , Lin Qiu , Shize Li , Yuqi Yan , Yang Ding , Yuhao Zhu , Fugen Wu , Xianbao Shi , Junzhao Zhao , Ruijie Chen , Qing Yao , Longfa Kou
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引用次数: 0

摘要

在辅助生殖技术中,卵母细胞在体外成熟(IVM)过程中会遇到高水平的活性氧(ROS)。氧化应激会对卵母细胞的质量产生不利影响,阻碍其成熟、生长和后续发育。因此,在体外成熟过程中减少过量的 ROS 以保护存活率较低的卵母细胞是一种可行的策略。许多抗氧化剂已被用于卵母细胞体外受精,并取得了可观的效果;然而,包括溶解性、稳定性和安全性在内的几个方面需要关注和解决。在这项研究中,我们通过自组装内源性胆红素和褪黑素激素,开发了涂有胆红素共轭乙二醇壳聚糖(MB@GBn)的纳米颗粒,以缓解氧化应激并促进卵母细胞成熟。优化后的 MB@GBn 呈均匀球形,粒径为 128 nm,PDI 值为 0.1807,表面电位为 +11.35 mV。带正电荷的电位有助于纳米粒子通过静电作用附着在卵母细胞表面,从而发挥功能作用。体外研究表明,MB@GB 能显著促进受损卵母细胞的成熟。进一步的研究表明,MB@GB 在清除 ROS、降低细胞内钙含量和抑制线粒体极化方面具有功效。这项研究不仅为生物医学应用中的纳米药物输送系统提供了一个新的视角,还提出了一种增强卵母细胞IVM的创新策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Enhancing oocyte in vitro maturation and quality by melatonin/bilirubin cationic nanoparticles: A promising strategy for assisted reproduction techniques

Enhancing oocyte in vitro maturation and quality by melatonin/bilirubin cationic nanoparticles: A promising strategy for assisted reproduction techniques

In assisted reproduction techniques, oocytes encounter elevated levels of reactive oxygen species (ROS) during in vitro maturation (IVM). Oxidative stress adversely affects oocyte quality, hampering their maturation, growth, and subsequent development. Thus, mitigating excessive ROS to safeguard less viable oocytes during IVM stands as a viable strategy. Numerous antioxidants have been explored for oocyte IVM, yielding considerable effects; however, several aspects, including solubility, stability, and safety, demand attention and resolution. In this study, we developed nanoparticles by self-assembling endogenous bilirubin and melatonin hormone coated with bilirubin-conjugated glycol chitosan (MB@GBn) to alleviate oxidative stress and enhance oocyte maturation. The optimized MB@GBn exhibited a uniform spherical shape, measuring 128 nm in particle size, with a PDI value of 0.1807 and a surface potential of +11.35 mV. The positively charged potential facilitated nanoparticle adherence to the oocyte surface through electrostatic interaction, allowing for functional action. In vitro studies demonstrated that MB@GB significantly enhanced the maturation of compromised oocytes. Further investigation revealed MB@GB's effectiveness in scavenging ROS, reducing intracellular calcium levels, and suppressing mitochondrial polarization. This study not only offers a novel perspective on nano drug delivery systems for biomedical applications but also presents an innovative strategy for enhancing oocyte IVM.

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来源期刊
International Journal of Pharmaceutics: X
International Journal of Pharmaceutics: X Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
6.60
自引率
0.00%
发文量
32
审稿时长
24 days
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