{"title":"采用微生物学调查方法了解医疗保健池中假单胞菌物种的毒力因素。","authors":"","doi":"10.1016/j.jhin.2024.06.012","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Hospital water is involved in both the prevention and spread of healthcare-associated infections (HCAIs). Handwashing is key to reducing the transmission of pathogens, yet numerous outbreaks have been found to be caused by organisms within sinks, taps and showers. <em>Pseudomonas aeruginosa</em> and increasingly non-<em>aeruginosa Pseudomonas</em> cause waterborne HCAI, however, little is known about the virulence potential of <em>Pseudomonas</em> species found within hospital environments.</p></div><div><h3>Methods</h3><p>Swabs were taken from 62 sinks within two newly opened wards at Great Ormond Street Hospital, samples were taken before and after the wards opened to understand the impact of patient occupancy on sink micro-organisms. Culturable bacteria were identified by MALDI-TOF and virulence factors assessed through phenotypic methods.</p></div><div><h3>Results</h3><p>A total of 106 bacterial isolates were recovered including 24 <em>Pseudomonas</em> isolates. Of these 25% were identified as <em>P. oleovorans</em>, 21% <em>P. aeruginosa</em>, 17% <em>P. composti</em>, 13% <em>P. alicalipha</em>, 8% <em>P. monteilii</em>, 4% <em>P. putida</em>, 4% <em>P. stutzeri</em> and 8% could only be identified to genus level by MALDI-TOF. Differences were seen in both the number of <em>Pseudomonas</em> isolates and virulence production between the two wards, overall 25% of the <em>Pseudomonas</em> isolates produced pigment, 58% were capable of haemolysis, 87.5% were able to swim, 83.3% were capable of twitching motility, 33.3% produced alkaline protease and 8.3% produced gelatinase.</p></div><div><h3>Conclusions</h3><p>Results suggest that patients may be back-contaminating sinks with colonizing organisms which has ongoing implications for infection prevention and control. Additionally, this work highlights the ability of non-<em>aeruginosa Pseudomonas</em> to produce virulence factors traditionally associated with <em>P. aeruginosa</em>.</p></div>","PeriodicalId":54806,"journal":{"name":"Journal of Hospital Infection","volume":"151 ","pages":"Pages 84-91"},"PeriodicalIF":3.9000,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0195670124002330/pdfft?md5=f52f0056b481939d85ad8446bfa1374e&pid=1-s2.0-S0195670124002330-main.pdf","citationCount":"0","resultStr":"{\"title\":\"A microbiological survey approach to understanding the virulence factors of Pseudomonas species in healthcare sinks\",\"authors\":\"\",\"doi\":\"10.1016/j.jhin.2024.06.012\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Hospital water is involved in both the prevention and spread of healthcare-associated infections (HCAIs). Handwashing is key to reducing the transmission of pathogens, yet numerous outbreaks have been found to be caused by organisms within sinks, taps and showers. <em>Pseudomonas aeruginosa</em> and increasingly non-<em>aeruginosa Pseudomonas</em> cause waterborne HCAI, however, little is known about the virulence potential of <em>Pseudomonas</em> species found within hospital environments.</p></div><div><h3>Methods</h3><p>Swabs were taken from 62 sinks within two newly opened wards at Great Ormond Street Hospital, samples were taken before and after the wards opened to understand the impact of patient occupancy on sink micro-organisms. Culturable bacteria were identified by MALDI-TOF and virulence factors assessed through phenotypic methods.</p></div><div><h3>Results</h3><p>A total of 106 bacterial isolates were recovered including 24 <em>Pseudomonas</em> isolates. Of these 25% were identified as <em>P. oleovorans</em>, 21% <em>P. aeruginosa</em>, 17% <em>P. composti</em>, 13% <em>P. alicalipha</em>, 8% <em>P. monteilii</em>, 4% <em>P. putida</em>, 4% <em>P. stutzeri</em> and 8% could only be identified to genus level by MALDI-TOF. Differences were seen in both the number of <em>Pseudomonas</em> isolates and virulence production between the two wards, overall 25% of the <em>Pseudomonas</em> isolates produced pigment, 58% were capable of haemolysis, 87.5% were able to swim, 83.3% were capable of twitching motility, 33.3% produced alkaline protease and 8.3% produced gelatinase.</p></div><div><h3>Conclusions</h3><p>Results suggest that patients may be back-contaminating sinks with colonizing organisms which has ongoing implications for infection prevention and control. Additionally, this work highlights the ability of non-<em>aeruginosa Pseudomonas</em> to produce virulence factors traditionally associated with <em>P. aeruginosa</em>.</p></div>\",\"PeriodicalId\":54806,\"journal\":{\"name\":\"Journal of Hospital Infection\",\"volume\":\"151 \",\"pages\":\"Pages 84-91\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-07-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0195670124002330/pdfft?md5=f52f0056b481939d85ad8446bfa1374e&pid=1-s2.0-S0195670124002330-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Hospital Infection\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0195670124002330\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Hospital Infection","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0195670124002330","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
A microbiological survey approach to understanding the virulence factors of Pseudomonas species in healthcare sinks
Background
Hospital water is involved in both the prevention and spread of healthcare-associated infections (HCAIs). Handwashing is key to reducing the transmission of pathogens, yet numerous outbreaks have been found to be caused by organisms within sinks, taps and showers. Pseudomonas aeruginosa and increasingly non-aeruginosa Pseudomonas cause waterborne HCAI, however, little is known about the virulence potential of Pseudomonas species found within hospital environments.
Methods
Swabs were taken from 62 sinks within two newly opened wards at Great Ormond Street Hospital, samples were taken before and after the wards opened to understand the impact of patient occupancy on sink micro-organisms. Culturable bacteria were identified by MALDI-TOF and virulence factors assessed through phenotypic methods.
Results
A total of 106 bacterial isolates were recovered including 24 Pseudomonas isolates. Of these 25% were identified as P. oleovorans, 21% P. aeruginosa, 17% P. composti, 13% P. alicalipha, 8% P. monteilii, 4% P. putida, 4% P. stutzeri and 8% could only be identified to genus level by MALDI-TOF. Differences were seen in both the number of Pseudomonas isolates and virulence production between the two wards, overall 25% of the Pseudomonas isolates produced pigment, 58% were capable of haemolysis, 87.5% were able to swim, 83.3% were capable of twitching motility, 33.3% produced alkaline protease and 8.3% produced gelatinase.
Conclusions
Results suggest that patients may be back-contaminating sinks with colonizing organisms which has ongoing implications for infection prevention and control. Additionally, this work highlights the ability of non-aeruginosa Pseudomonas to produce virulence factors traditionally associated with P. aeruginosa.
期刊介绍:
The Journal of Hospital Infection is the editorially independent scientific publication of the Healthcare Infection Society. The aim of the Journal is to publish high quality research and information relating to infection prevention and control that is relevant to an international audience.
The Journal welcomes submissions that relate to all aspects of infection prevention and control in healthcare settings. This includes submissions that:
provide new insight into the epidemiology, surveillance, or prevention and control of healthcare-associated infections and antimicrobial resistance in healthcare settings;
provide new insight into cleaning, disinfection and decontamination;
provide new insight into the design of healthcare premises;
describe novel aspects of outbreaks of infection;
throw light on techniques for effective antimicrobial stewardship;
describe novel techniques (laboratory-based or point of care) for the detection of infection or antimicrobial resistance in the healthcare setting, particularly if these can be used to facilitate infection prevention and control;
improve understanding of the motivations of safe healthcare behaviour, or describe techniques for achieving behavioural and cultural change;
improve understanding of the use of IT systems in infection surveillance and prevention and control.