评估用于检测鼻咽拭子和痰标本中呼吸道病原体的 FPMC 呼吸系统面板。

IF 4 3区医学 Q2 VIROLOGY

Virology Journal Pub Date : 2024-07-11 DOI:10.1186/s12985-024-02430-x

Li Xue, Jianhong Zhu, Ke Lei, Zeshi Liu, Yiwei Tang, Bo Zhong, Ning Gao, Chaoliang Xiong, Jing Lei, Ying Tian, Weixiao Zhou, Nan Feng, Xue Zhang, Dong Chen, Jing Li, Yan Geng

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引用次数: 0

摘要

检测目的评估新型呼吸道病原体检测板（荧光探针熔解曲线，FPMC）定性检测 12 种病原体（肺炎衣原体、肺炎支原体、腺病毒、甲型流感病毒、乙型流感病毒、副流感病毒、鼻病毒等）的性能：方法：对前瞻性采集的鼻咽拭子（NPS）和痰标本（n = 635）使用 FPMC 面板进行检测，并以 Sanger 测序法作为比较方法：在NPS和痰标本中，FPMC分析方法与Sanger测序方法的总体一致性分别为100%和99.66%。经检测，FPMC 分析法与桑格测序法对鼻咽癌和痰标本的总体阳性符合率均为 100%。FPMC 分析方法还证明，对无指纹图谱和痰标本的总体阴性吻合率分别为 100%和 99.38%：FPMC分析法是一种稳定、准确的检测方法，可用于快速、全面地检测呼吸道病原体。

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Evaluation of the FPMC respiratory panel for detection of respiratory tract pathogens in nasopharyngeal swab and sputum specimens.

Objectives: The performance of the new Respiratory Pathogen panel (fluorescent probe melting curve, FPMC) for the qualitative detection of 12 organisms (chlamydia pneumoniae, mycoplasma pneumoniae, adenovirus, influenza A virus, influenza B virus, parainfluenza virus, rhinovirus, etc.) was assessed.

Methods: Prospectively collected nasopharyngeal swab (NPS) and sputum specimens (n = 635) were detected by using the FPMC panel, with the Sanger sequencing method as the comparative method.

Results: The overall percent concordance between the FPMC analysis method and the Sanger sequencing method was 100% and 99.66% for NPS and sputum specimens, respectively. The FPMC testified an overall positive percent concordance of 100% for both NPS and sputum specimens. The FPMC analysis method also testified an overall negative percent concordance of 100% and 99.38% for NPS and sputum specimens, respectively.

Conclusions: The FPMC analysis method is a stable and accurate assay for rapid, comprehensive detecting for respiratory pathogens.

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来源期刊

Virology Journal 医学-病毒学

CiteScore

7.40

自引率

2.10%

发文量

186

审稿时长

1 months

期刊介绍： Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies. The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.