Naseema Bee Shabulal, Perumal Thiyagarajan, Mohammed F. Albeshr, Karuppiah Nagaraj, Gunasekaran Velmurugan, Prasenjit Maity, Isai Mathivanan, S. M. Abhijith and Niyati Hemantkumar Lad
{"title":"银纳米粒子的绿色合成:了解生物活性--抗癌、抗菌和通过释放活性氧裂解质粒 DNA","authors":"Naseema Bee Shabulal, Perumal Thiyagarajan, Mohammed F. Albeshr, Karuppiah Nagaraj, Gunasekaran Velmurugan, Prasenjit Maity, Isai Mathivanan, S. M. Abhijith and Niyati Hemantkumar Lad","doi":"10.1039/D4NJ02350A","DOIUrl":null,"url":null,"abstract":"<p >This study is designed to explore the preparation, characterization, biological impacts, and DNA degradation abilities of green synthesized NPs. The UV-visible analysis confirms the reduction of Ag along with pH. FTIR studies are used to determine the functional groups involved in the reduction reaction. Moreover, HR-TEM and XRD reveal the morphology and crystal plane of the NPs. Further, the XPS study confirms the elemental analysis and oxidation state of the metal. Biological studies were conducted to assess the effectiveness of NPs, including their antibacterial, anticancer, and DNA degradation activities. According to the acquired data, Ag-NPs show a strong antibacterial action against both Gram-positive and Gram-negative bacteria. Additionally, Ag-NPs demonstrate cytotoxicity against pBR322 DNA degradation and breast cancer cell lines at 40 and 50 μg mL<small><sup>−1</sup></small>, respectively. Herewith, we report a simple synthesis of Ag-NPs with benign, environmentally safe, biological reduction by using natural biomolecules. The synthesized Ag-NPs have a size around 10 nm and the ability to produce ROS under visible light irradiation. Finally, Ag-NPs exhibit cytotoxicity, including intracellular ROS generation, cell death, loss of mitochondrial potential, and DNA fragmentation on breast cancer cells. Importantly, Ag-NPs show nuclease activity.</p>","PeriodicalId":95,"journal":{"name":"New Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Green synthesis of silver nanoparticles: understanding biological activities – anticancer, antimicrobial, and plasmid DNA cleavage properties through reactive oxygen species release†\",\"authors\":\"Naseema Bee Shabulal, Perumal Thiyagarajan, Mohammed F. Albeshr, Karuppiah Nagaraj, Gunasekaran Velmurugan, Prasenjit Maity, Isai Mathivanan, S. M. Abhijith and Niyati Hemantkumar Lad\",\"doi\":\"10.1039/D4NJ02350A\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >This study is designed to explore the preparation, characterization, biological impacts, and DNA degradation abilities of green synthesized NPs. The UV-visible analysis confirms the reduction of Ag along with pH. FTIR studies are used to determine the functional groups involved in the reduction reaction. Moreover, HR-TEM and XRD reveal the morphology and crystal plane of the NPs. Further, the XPS study confirms the elemental analysis and oxidation state of the metal. Biological studies were conducted to assess the effectiveness of NPs, including their antibacterial, anticancer, and DNA degradation activities. According to the acquired data, Ag-NPs show a strong antibacterial action against both Gram-positive and Gram-negative bacteria. Additionally, Ag-NPs demonstrate cytotoxicity against pBR322 DNA degradation and breast cancer cell lines at 40 and 50 μg mL<small><sup>−1</sup></small>, respectively. Herewith, we report a simple synthesis of Ag-NPs with benign, environmentally safe, biological reduction by using natural biomolecules. The synthesized Ag-NPs have a size around 10 nm and the ability to produce ROS under visible light irradiation. Finally, Ag-NPs exhibit cytotoxicity, including intracellular ROS generation, cell death, loss of mitochondrial potential, and DNA fragmentation on breast cancer cells. Importantly, Ag-NPs show nuclease activity.</p>\",\"PeriodicalId\":95,\"journal\":{\"name\":\"New Journal of Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-07-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"New Journal of Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2024/nj/d4nj02350a\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Journal of Chemistry","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/nj/d4nj02350a","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
摘要
本研究旨在探索绿色合成 NPs 的制备、表征、生物影响和 DNA 降解能力。紫外-可见光分析证实了 Ag 随着 pH 值的变化而还原。傅立叶变换红外光谱研究用于确定参与还原反应的官能团。此外,HR-TEM 和 XRD 揭示了 NPs 的形态和晶面。此外,XPS 研究证实了元素分析和金属的氧化态。另一方面,为了实现 NPs 的卓越性能,如抗菌、抗癌和 DNA 降解活性,还进行了生物学研究。研究结果表明,Ag-NPs 对革兰氏阴性菌和革兰氏阳性菌具有良好的抗菌效果。此外,Ag-NPs 对乳腺癌细胞株具有细胞毒性(分别为 40 和 50 μg/ml),并能降解 pBR322 DNA。在此,我们报告了一种利用天然生物大分子简单合成Ag-NPs的方法,该方法具有良性、环境安全、生物还原等特点。合成的 Ag-NPs 大小约为 10 nm,在可见光照射下能产生 ROS。最后,Ag-NPs 对乳腺癌细胞具有细胞毒性,包括细胞内 ROS 生成、细胞死亡、线粒体电位丧失和 DNA 断裂。重要的是,Ag-NPs 还具有核酸酶活性。
Green synthesis of silver nanoparticles: understanding biological activities – anticancer, antimicrobial, and plasmid DNA cleavage properties through reactive oxygen species release†
This study is designed to explore the preparation, characterization, biological impacts, and DNA degradation abilities of green synthesized NPs. The UV-visible analysis confirms the reduction of Ag along with pH. FTIR studies are used to determine the functional groups involved in the reduction reaction. Moreover, HR-TEM and XRD reveal the morphology and crystal plane of the NPs. Further, the XPS study confirms the elemental analysis and oxidation state of the metal. Biological studies were conducted to assess the effectiveness of NPs, including their antibacterial, anticancer, and DNA degradation activities. According to the acquired data, Ag-NPs show a strong antibacterial action against both Gram-positive and Gram-negative bacteria. Additionally, Ag-NPs demonstrate cytotoxicity against pBR322 DNA degradation and breast cancer cell lines at 40 and 50 μg mL−1, respectively. Herewith, we report a simple synthesis of Ag-NPs with benign, environmentally safe, biological reduction by using natural biomolecules. The synthesized Ag-NPs have a size around 10 nm and the ability to produce ROS under visible light irradiation. Finally, Ag-NPs exhibit cytotoxicity, including intracellular ROS generation, cell death, loss of mitochondrial potential, and DNA fragmentation on breast cancer cells. Importantly, Ag-NPs show nuclease activity.