{"title":"通过诱导肝癌细胞凋亡从麦角冬虫夏草 OS8 栽培菌丝体中提取的β-D-葡聚糖多糖的抗癌潜力","authors":"Suwannachom Chatnarin, Mongkol Thirabunyanon","doi":"10.1016/j.procbio.2024.07.009","DOIUrl":null,"url":null,"abstract":"<div><p>The fungus <em>Ophiocordyceps sinensis</em> OS8 has been sourced from the wild variant of <em>O. sinensis</em>. In this study, the enriched β-D-glucan polysaccharide obtained from cultured mycelium of <em>O. sinensis</em> OS8 (OS8P) was examined for its potential to inhibit the growth of HepG2 liver cancer cells, and the IC<sub>50</sub> was 511.79 µg/mL. The study of cellular migration was analyzed by scratch assay. It was found that the HepG2 cell migration was significantly inhibited (<em>P</em> < 0.05) in the OS8P-treated group. The cell apoptotic feature’s reliability was determined via DAPI staining to confirm the experimental results' reliability. The AO/PI double staining assay showed that HepG2 cells treated with OS8P (IC<sub>50</sub>) had dead cells with condensed and shrieking nuclei that are late apoptotic. Morphological alterations were meticulously examined using scanning electron microscopy. The specific morphological characteristics of apoptotic cells, notably cell lysis, were distinctly observable. The damaged cells demonstrated severe structural disruption, loss of original shape, blebs formation, and apoptotic bodies' appearance. These findings provide pharmacological proof that the OS8P extract could be potentially employed in the food and medical industries for anticancer applications.</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Potential of β-D-glucan polysaccharide from Ophiocordyceps sinensis OS8 cultivated mycelium on anticancer activity via inducing liver cancer cell death apoptosis\",\"authors\":\"Suwannachom Chatnarin, Mongkol Thirabunyanon\",\"doi\":\"10.1016/j.procbio.2024.07.009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The fungus <em>Ophiocordyceps sinensis</em> OS8 has been sourced from the wild variant of <em>O. sinensis</em>. In this study, the enriched β-D-glucan polysaccharide obtained from cultured mycelium of <em>O. sinensis</em> OS8 (OS8P) was examined for its potential to inhibit the growth of HepG2 liver cancer cells, and the IC<sub>50</sub> was 511.79 µg/mL. The study of cellular migration was analyzed by scratch assay. It was found that the HepG2 cell migration was significantly inhibited (<em>P</em> < 0.05) in the OS8P-treated group. The cell apoptotic feature’s reliability was determined via DAPI staining to confirm the experimental results' reliability. The AO/PI double staining assay showed that HepG2 cells treated with OS8P (IC<sub>50</sub>) had dead cells with condensed and shrieking nuclei that are late apoptotic. Morphological alterations were meticulously examined using scanning electron microscopy. The specific morphological characteristics of apoptotic cells, notably cell lysis, were distinctly observable. The damaged cells demonstrated severe structural disruption, loss of original shape, blebs formation, and apoptotic bodies' appearance. These findings provide pharmacological proof that the OS8P extract could be potentially employed in the food and medical industries for anticancer applications.</p></div>\",\"PeriodicalId\":20811,\"journal\":{\"name\":\"Process Biochemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-07-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Process Biochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1359511324002319\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Process Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1359511324002319","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Potential of β-D-glucan polysaccharide from Ophiocordyceps sinensis OS8 cultivated mycelium on anticancer activity via inducing liver cancer cell death apoptosis
The fungus Ophiocordyceps sinensis OS8 has been sourced from the wild variant of O. sinensis. In this study, the enriched β-D-glucan polysaccharide obtained from cultured mycelium of O. sinensis OS8 (OS8P) was examined for its potential to inhibit the growth of HepG2 liver cancer cells, and the IC50 was 511.79 µg/mL. The study of cellular migration was analyzed by scratch assay. It was found that the HepG2 cell migration was significantly inhibited (P < 0.05) in the OS8P-treated group. The cell apoptotic feature’s reliability was determined via DAPI staining to confirm the experimental results' reliability. The AO/PI double staining assay showed that HepG2 cells treated with OS8P (IC50) had dead cells with condensed and shrieking nuclei that are late apoptotic. Morphological alterations were meticulously examined using scanning electron microscopy. The specific morphological characteristics of apoptotic cells, notably cell lysis, were distinctly observable. The damaged cells demonstrated severe structural disruption, loss of original shape, blebs formation, and apoptotic bodies' appearance. These findings provide pharmacological proof that the OS8P extract could be potentially employed in the food and medical industries for anticancer applications.
期刊介绍:
Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.