Sheng-Nan Sun , Lindsay L. Fan , Aipo Diao , Zhen-Chuan Fan
{"title":"衣藻产生的三重欧氏防御素通过破坏细胞膜完整性抑制细菌生长","authors":"Sheng-Nan Sun , Lindsay L. Fan , Aipo Diao , Zhen-Chuan Fan","doi":"10.1016/j.procbio.2024.06.019","DOIUrl":null,"url":null,"abstract":"<div><p>Oh-defensin, an antimicrobial peptide, extracted from the venom of <em>Ornithoctonus hainana</em>, potently inhibited fungi and Gram-negative bacteria but showed limited antibacterial potency against Gram-positive bacteria via an unknown mechanism. In this study, a recombinant peptide termed as 3×Oh-defensin-HA-6×His composed of three repeats of Oh-defensin followed by hemagglutinin (HA) and six histidine (6×His) double tags was expressed in <em>Chlamydomonas reinhardtii</em>. The bacteriostatic activity of 3×Oh-defensin-HA-6×His certified against both Gram-negative and Gram-positive bacteria was confirmed, with minimum inhibitory concentration (MIC) between 10 and 80 µg/mL. The minimum bactericidal concentration (MBC) was determined to range from 1× to 2×MIC. In addition, 3×Oh-defensin-HA-6×His exhibited resistance to temperature shocks, extreme pH, and proteinase digestion and it was biologically safe because it displayed low cytotoxicity and hemolysis. In addition, scanning electron microscopy (SEM) and propidium iodide (PI) staining revealed that 3×Oh-defensin-HA-6×His caused the destruction of the bacterial cell membrane, which effectively inhibited bacterial growth. In summary, our findings suggest that green algae-derived 3×Oh-defensin-HA-6×His holds significant promise as a potential alternative to conventional antibiotics in the future.</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chlamydomonas reinhardtii-derived triple Oh-defensin inhibits the growth of bacteria by disrupting cell membrane integrity\",\"authors\":\"Sheng-Nan Sun , Lindsay L. Fan , Aipo Diao , Zhen-Chuan Fan\",\"doi\":\"10.1016/j.procbio.2024.06.019\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Oh-defensin, an antimicrobial peptide, extracted from the venom of <em>Ornithoctonus hainana</em>, potently inhibited fungi and Gram-negative bacteria but showed limited antibacterial potency against Gram-positive bacteria via an unknown mechanism. In this study, a recombinant peptide termed as 3×Oh-defensin-HA-6×His composed of three repeats of Oh-defensin followed by hemagglutinin (HA) and six histidine (6×His) double tags was expressed in <em>Chlamydomonas reinhardtii</em>. The bacteriostatic activity of 3×Oh-defensin-HA-6×His certified against both Gram-negative and Gram-positive bacteria was confirmed, with minimum inhibitory concentration (MIC) between 10 and 80 µg/mL. The minimum bactericidal concentration (MBC) was determined to range from 1× to 2×MIC. In addition, 3×Oh-defensin-HA-6×His exhibited resistance to temperature shocks, extreme pH, and proteinase digestion and it was biologically safe because it displayed low cytotoxicity and hemolysis. In addition, scanning electron microscopy (SEM) and propidium iodide (PI) staining revealed that 3×Oh-defensin-HA-6×His caused the destruction of the bacterial cell membrane, which effectively inhibited bacterial growth. In summary, our findings suggest that green algae-derived 3×Oh-defensin-HA-6×His holds significant promise as a potential alternative to conventional antibiotics in the future.</p></div>\",\"PeriodicalId\":20811,\"journal\":{\"name\":\"Process Biochemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-06-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Process Biochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1359511324002058\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Process Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1359511324002058","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Chlamydomonas reinhardtii-derived triple Oh-defensin inhibits the growth of bacteria by disrupting cell membrane integrity
Oh-defensin, an antimicrobial peptide, extracted from the venom of Ornithoctonus hainana, potently inhibited fungi and Gram-negative bacteria but showed limited antibacterial potency against Gram-positive bacteria via an unknown mechanism. In this study, a recombinant peptide termed as 3×Oh-defensin-HA-6×His composed of three repeats of Oh-defensin followed by hemagglutinin (HA) and six histidine (6×His) double tags was expressed in Chlamydomonas reinhardtii. The bacteriostatic activity of 3×Oh-defensin-HA-6×His certified against both Gram-negative and Gram-positive bacteria was confirmed, with minimum inhibitory concentration (MIC) between 10 and 80 µg/mL. The minimum bactericidal concentration (MBC) was determined to range from 1× to 2×MIC. In addition, 3×Oh-defensin-HA-6×His exhibited resistance to temperature shocks, extreme pH, and proteinase digestion and it was biologically safe because it displayed low cytotoxicity and hemolysis. In addition, scanning electron microscopy (SEM) and propidium iodide (PI) staining revealed that 3×Oh-defensin-HA-6×His caused the destruction of the bacterial cell membrane, which effectively inhibited bacterial growth. In summary, our findings suggest that green algae-derived 3×Oh-defensin-HA-6×His holds significant promise as a potential alternative to conventional antibiotics in the future.
期刊介绍:
Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.