在微污染物浓度逐渐增加的情况下,对移动床生物膜反应器载体上的活性微生物群进行总 RNA 分析。

IF 3.5 3区 生物学 Q2 MICROBIOLOGY
Joseph Donald Martin, Selina Tisler, Maria Scheel, Sif Svendsen, Muhammad Zohaib Anwar, Athanasios Zervas, Flemming Ekelund, Kai Bester, Lars Hestbjerg Hansen, Carsten Suhr Jacobsen, Lea Ellegaard-Jensen
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引用次数: 0

摘要

微污染物在水生环境中越来越普遍。由于传统的处理技术无法充分去除微污染物,因此这些微污染物主要来自废水处理厂。然而,移动床生物膜反应器(MBBRs)作为一个抛光步骤应用于传统废水处理时,已被证明有助于去除微污染物。在此,我们利用总 RNA 测序技术研究了移动床生物膜反应器生物膜在微污染物浓度不断增加的情况下的活性微生物群落和功能动态。同时,我们使用 MBBR 生物膜载体进行了批量培养实验,以评估微污染物的降解潜力。我们的研究表明,生物膜真核生物,尤其是原生动物,受到微污染物暴露的负面影响,而相对丰度增加的原核生物则相反。此外,我们还发现,在添加了微污染物的 MBBR 和对照组之间,有几个功能基因的表达存在差异。其中包括参与芳香族和异生物化合物降解的基因。此外,生物膜载体批次实验表明,随着 MBBR 中微污染物浓度的增加,苯并三唑和双氯芬酸的降解发生了巨大的变化。最终,这项研究提供了有关 MBBR 微生物群落和功能动态以及微污染物负荷增加如何影响这些动态的重要见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Total RNA analysis of the active microbiome on moving bed biofilm reactor carriers under incrementally increasing micropollutant concentrations.

Micropollutants are increasingly prevalent in the aquatic environment. A major part of these originates from wastewater treatment plants since traditional treatment technologies do not remove micropollutants sufficiently. Moving bed biofilm reactors (MBBRs), however, have been shown to aid in micropollutant removal when applied to conventional wastewater treatment as a polishing step. Here, we used Total RNA sequencing to investigate both the active microbial community and functional dynamics of MBBR biofilms when these were exposed to increasing micropollutant concentrations over time. Concurrently, we conducted batch culture experiments using biofilm carriers from the MBBRs to assess micropollutant degradation potential. Our study showed that biofilm eukaryotes, in particular protozoa, were negatively influenced by micropollutant exposure, in contrast to prokaryotes that increased in relative abundance. Further, we found several functional genes that were differentially expressed between the MBBR with added micropollutants and the control. These include genes involved in aromatic and xenobiotic compound degradation. Moreover, the biofilm carrier batch experiment showed vastly different alterations in benzotriazole and diclofenac degradation following the increased micropollutant concentrations in the MBBR. Ultimately, this study provides essential insights into the microbial community and functional dynamics of MBBRs and how an increased load of micropollutants influences these dynamics.

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来源期刊
FEMS microbiology ecology
FEMS microbiology ecology 生物-微生物学
CiteScore
7.50
自引率
2.40%
发文量
132
审稿时长
3 months
期刊介绍: FEMS Microbiology Ecology aims to ensure efficient publication of high-quality papers that are original and provide a significant contribution to the understanding of microbial ecology. The journal contains Research Articles and MiniReviews on fundamental aspects of the ecology of microorganisms in natural soil, aquatic and atmospheric habitats, including extreme environments, and in artificial or managed environments. Research papers on pure cultures and in the areas of plant pathology and medical, food or veterinary microbiology will be published where they provide valuable generic information on microbial ecology. Papers can deal with culturable and non-culturable forms of any type of microorganism: bacteria, archaea, filamentous fungi, yeasts, protozoa, cyanobacteria, algae or viruses. In addition, the journal will publish Perspectives, Current Opinion and Controversy Articles, Commentaries and Letters to the Editor on topical issues in microbial ecology. - Application of ecological theory to microbial ecology - Interactions and signalling between microorganisms and with plants and animals - Interactions between microorganisms and their physicochemical enviornment - Microbial aspects of biogeochemical cycles and processes - Microbial community ecology - Phylogenetic and functional diversity of microbial communities - Evolutionary biology of microorganisms
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