微藻类和蓝藻耐强光的底盘工程。

IF 8.1 2区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biyun Dou, Yang Li, Fangzhong Wang, Lei Chen, Weiwen Zhang
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引用次数: 0

摘要

微藻类和蓝藻的含氧光合作用被认为是加速能源转型和减缓全球变暖的重要底盘。目前,用于大规模应用的光合微生物培养系统遇到了过度光照的压力。强光胁迫会:影响光合效率、降低生产力、限制细胞生长,甚至导致细胞死亡。破译光保护机制和构建耐强光底盘是近年来的研究重点。在本综述中,我们首先简要介绍常见微藻和蓝藻应对强光胁迫的自我保护机制。这些机制主要包括:避免过量光吸收、耗散过量激发能、淬灭过量高能电子、ROS解毒和PSII修复。我们将重点介绍这些机制的物种特异性差异以及最新进展。然后,我们回顾了创建高耐光性底盘的工程策略,例如:缩小光收集天线的尺寸、优化非光化学淬灭、优化光合电子传递以及增强 PSII 修复。最后,我们建议对以下机制进行全面探索:已确定的高耐光性底盘的基础机制、利用创新方法鉴定与高耐光性相关的新基因、利用 CRISPR 系统和人工智能进行底盘工程改造,以及引入植物光保护机制作为未来的研究方向。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chassis engineering for high light tolerance in microalgae and cyanobacteria.

Oxygenic photosynthesis in microalgae and cyanobacteria is considered an important chassis to accelerate energy transition and mitigate global warming. Currently, cultivation systems for photosynthetic microbes for large-scale applications encountered excessive light exposure stress. High light stress can: affect photosynthetic efficiency, reduce productivity, limit cell growth, and even cause cell death. Deciphering photoprotection mechanisms and constructing high-light tolerant chassis have been recent research focuses. In this review, we first briefly introduce the self-protection mechanisms of common microalgae and cyanobacteria in response to high light stress. These mechanisms mainly include: avoiding excess light absorption, dissipating excess excitation energy, quenching excessive high-energy electrons, ROS detoxification, and PSII repair. We focus on the species-specific differences in these mechanisms as well as recent advancements. Then, we review engineering strategies for creating high-light tolerant chassis, such as: reducing the size of the light-harvesting antenna, optimizing non-photochemical quenching, optimizing photosynthetic electron transport, and enhancing PSII repair. Finally, we propose a comprehensive exploration of mechanisms: underlying identified high light tolerant chassis, identification of new genes pertinent to high light tolerance using innovative methodologies, harnessing CRISPR systems and artificial intelligence for chassis engineering modification, and introducing plant photoprotection mechanisms as future research directions.

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来源期刊
Critical Reviews in Biotechnology
Critical Reviews in Biotechnology 工程技术-生物工程与应用微生物
CiteScore
20.80
自引率
1.10%
发文量
71
审稿时长
4.8 months
期刊介绍: Biotechnological techniques, from fermentation to genetic manipulation, have become increasingly relevant to the food and beverage, fuel production, chemical and pharmaceutical, and waste management industries. Consequently, academic as well as industrial institutions need to keep abreast of the concepts, data, and methodologies evolved by continuing research. This journal provides a forum of critical evaluation of recent and current publications and, periodically, for state-of-the-art reports from various geographic areas around the world. Contributing authors are recognized experts in their fields, and each article is reviewed by an objective expert to ensure accuracy and objectivity of the presentation.
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